LOCUS       sequence-223365-        4963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector construction module. Partition genes (parABCDE) for plasmid 
            stability in absence of antibiotic selection for construction of 
            Golden Gate cloning vectors .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG012
FEATURES             Location/Qualifiers
     source          1..4963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2953..2970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3063..3651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3143..3162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(4836..4940)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccatgag ctccactctg tgcgtctcat tactgcatga gcttgtggaa
      421 gtgtgctgag cataaatgat tctcctagct gttctttggg taagtacgcc atcaggacgt
      481 tgtgagtggc gcgattttta gcggctgaaa tcagcccttg agcctgtcgg caagtcgcgt
      541 catgaggtcc atgcgctcat gcaggatcgc cacgaccaac gcgggttcgc ccgcacgcgg
      601 caggcaaaaa acgtagtggt gttcgcagcg ggccatccgc agcgcgggaa agagttcgct
      661 catgtcctta aacgggcctt cgccggcggc aagcctggct atgccctgtt ccagcttagc
      721 gatatagcgg cgcacctgcg ccgcgcccca ctcccggcgc gtgtagcgga tgatgccgcg
      781 tagatcggct tcggcctcag ccgtgaggat gtaggccgtc aagcgcgatc cccgctgagt
      841 tcttcatcaa gaatttcgcc gacgctcttg gtggacacct tgccggcaag cccatcgttg
      901 atgcggttcc ccagcatggt tttcagttcc tgccatgcct gatcggcatc agcgtcaccg
      961 gggaacagac gttcgagggc gtattgctta atggtcttgc cctgcaaggc ggccagggct
     1021 ttcaggctct ggtgctgctg gtccgtcatg tcgattgtca ggcggctcat tggataacct
     1081 ccataaaata cacgtaacca cattagcaca tatgtgggcg tgaggctaca gcgcgaggcg
     1141 cattaaggtc gggaaaatgc gctaggcgca tttaaattgc gtattgctgt aatgcgccat
     1201 gccggctaga ctaggcccaa atgggtatac ccaatttgac caagggggac gcgatgaggg
     1261 cggccaagca ctaccgacaa cttctatcca tcgacttcaa catcgaggcg ctggccttcg
     1321 tgcctggacc cgacggcaca cgcggccggc gcatccacgt cctggggcgc gaggtccgcg
     1381 accggcccgg cctggtcgag tacctttcgc cggcgttcgg ctcgcgggtg gcgctggacg
     1441 gctactgcaa ggccaatttc gatgcagtgc tgcacctggc gtaccccgat catcagcaat
     1501 ggggccacgc atgaagcgcc gaagctacgc catgctgcgc gccgctgccg cgctggccgt
     1561 cctggtcgtt gcctcgccgg catgggccga gctgcgcggc gaggtcgtgc gcatcatcga
     1621 cggcgacacc atcgacgtgc tggtagacaa gcagccggtg cgcgtgcgcc tggtggacat
     1681 tgacgcgccg gaaaagcggc aagccttcgg cgaacgtgcg cgccaggcgc tggccggcat
     1741 ggtgttccgc cggcacgtcc tggtcgacga gaaggacacc gaccgttacg gccgcacgct
     1801 gggcaccgtc tgggtcaaca tggagctggc cagccggccg ccgcagccgc gcaacgtcaa
     1861 cgccgcgatg gttcaccagg gcatggcgtg ggcctatcgc ttccacggcc gcgcggccga
     1921 ccctgaaatg ctgcggctcg aacaggaggc gcgaggcaag cgcgtcggcc tctggtccga
     1981 tccgcacgcc gtcgagccgt ggaaatggcg acgcgagagc aacaaccgga gggacgaagg
     2041 ttgaaggtcg cccgcatcta cctgcgcgcc agtacggacg agcagaatct tgaacgccag
     2101 gagagccttg tagcggccac gcgggccgcc gggtactacg tcgccggcat ctaccgcgag
     2161 aaggcgtccg gcgcacgcgc cgaccggccc gagctgctgc gcatgatcgc ggacctgcaa
     2221 cctggtgaag tcgtcgttgc ggagaagatc gaccgcatca gccgcttgcc gttggccgag
     2281 gccgagcgcc tggttgcgtc gatccgggcc aaaggggcca agctggccgt gcctggcgtg
     2341 gtggacctgt cggagctggc cgccgaggcg aacggagtgg cgaaaatcgt tctggaatcc
     2401 gtccaggaca tgcttttgaa gctcgccttg cagatggccc gcgacgacta cgaggatcgg
     2461 cgcgagcgtc aacgtcaggg tgtccagttg gcgaaggccg ccggccgcta caccggccgc
     2521 aaacgtgacg ccggcatgca cgaccgcatc atcacgcttc gctccggcgg atcgagcatt
     2581 gccaagacgg ccaagctggt cggatgcagc ccgagccagg tcaaacgagt gtgggcggcc
     2641 tggaacgcgc agcagcaaaa ataaagccgg gcagtgcccg gcttttctca ccttttcgcg
     2701 tcccgcaggg ccgctgcgag cgccctacct agatcctcgc tttccccctc ggtgtagtcc
     2761 ggccagggca cgaagggcgc ggatgcgaac ctgttgagca ggtcagatga gacgcacgaa
     2821 gtgggtacct cttaattaac tggcctcatg ggccttcact tcactgcccg ctttccagtc
     2881 gggaaacctg tcgtgccagc tgcattaaca tggtcatagc tgtttccttg cgtattgggc
     2941 gctctccgct tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa agcctggggt
     3001 gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3061 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3661 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3781 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttattattt acccaccact
     3841 ttggtaattt cgcctttcac gtaatgcaca aattcttcca gctgatccgc acggctggcc
     3901 agacgatctt cttcctggcc cagatacgcc tgacgcgctt ccagaatcac cggctgatac
     3961 tgcgccggca gacgttccat ggcccaatcc gccgccacat ctttcggcgc aattttgccg
     4021 gtcaccgcgc tataccaaat acggctcagg gtcagcacca cgttgcgttc atcacccgcc
     4081 caatccggcg ggctgttcca cagggtcagg gtttcgttca gcgcttcaaa caggtcctgt
     4141 tccggcaccg gatcaaacag ttcttccgct gccggaccaa ccagcgccac gctatgttca
     4201 cgcgctttgg tcagcagaat ggccagatcg atatcaatgg tcgccggttc aaaaatgccg
     4261 gccagaatat cgttacgctg ccattcgcca aactgcagct cacgtttcgc cggataacgc
     4321 cacggaataa tgtcatcatg caccacgatg gtcacttcca ccgcacgcag aatttcgctt
     4381 tcacccgggc tcgcgctggt ttccagcaga tcgttaatca gcgcacgacg ggtggtttca
     4441 tccagacgca cggtcacggt caccagcaga tcaatatcgc tatgcggttt caggccgcca
     4501 tccaccgcgc tgccatacag atgcacggcc agcagggtcg gttccagatg acgttcaatc
     4561 acgcccacca cttcagacag ctgggtgctc acttccgcaa tcaccgcttc acgcataatg
     4621 ttcagtttgg ttttcggacg gctgccctgc tgggtcacat cgttgctgct ccacagcatc
     4681 aggcaacggc ccaccgcata acgcgcctgc tgtttgctcg cacgcggcat gctctgcacg
     4741 ccaaaaaagc aatcatagca cgccataaac accgccaccg caccattacc gccgctacgt
     4801 tcggtcaggg tacggctcca attacggcta cgcatactct tcctttttca atattattga
     4861 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4921 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
