LOCUS       sequence-22337-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  TITLE     Buchler lab plasmids
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p404CYC1
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     319..335
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     319..335
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(369..385)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(370..386)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      388..635
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(404..422)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(653..672)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(654..672)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(682..699)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(682..698)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(691..713)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctcactaaag ggaacaaaag ctggagctca tttggcgagc gttggttggt ggatcaagcc
       61 cacgcgtagg caatcctcga gcagatccgc caggcgtgta tatatagcgt ggatggccag
      121 gcaactttag tgctgacaca tacaggcata tatatatgtg tgcgacgaca catgatcata
      181 tggcatgcat gtgctctgta tgtatataaa actcttgttt tcttcttttc tctaaatatt
      241 ctttccttat acattaggac ctttgcagca taaattacta tacttctata gacacgcaaa
      301 cacaaataca cacactaatc tagaactagt ggatcccccg ggctgcagga attcgatatc
      361 aagcttatcg ataccgtcga cctcgagtca tgtaattagt tatgtcacgc ttacattcac
      421 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
      481 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
      541 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
      601 agaaggtttt gggacgctcg aaggctttaa tttgcggccg gtacccaatt cgccctatag
      661 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      721 tggcgttacc caacttaatc gccttgcagc acatcccctt tcgccagctg gcgta
//
