LOCUS       sequence-223372-        8270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 2 cloning site, position 1 module for vector 
            construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG006
FEATURES             Location/Qualifiers
     source          1..8270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1924..1933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      6019..6113
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6260..6277)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6370..6958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6450..6469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(8143..8247)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccacact ctgtgcgtct catgcctgcc ttgtcttctg gtcgactcga
      421 cggagggtac cgcacggtct gccaatccga cggaggttta tgaattttcc accttttcca
      481 caagctcaac tagtattaac gatgtggatt tagcaaaaaa aacctgtaac cctaaatgta
      541 aaataacggg taagcctgcc aaccatgtta tggcagatta agcgtctttt tgaagggcac
      601 cgcatctttc gcgttgccgt aaatgtatcc gtttataagg acagcccgaa tgacggtctg
      661 cgcaaaaaaa cacgttcatc tcactcgcga tgctgcggag cagttactgg ctgatattga
      721 tcgacgcctt gatcagttat tgcccgtgga gggagaacgg gatgttgtgg gtgccgcgat
      781 gcgtgaaggt gcgctggcac cgggaaaacg tattcgcccc atgttgctgt tgctgaccgc
      841 ccgcgatctg ggttgcgctg tcagccatga cggattactg gatttggcct gtgcggtgga
      901 aatggtccac gcggcttcgc tgatccttga cgatatgccc tgcatggacg atgcgaagct
      961 gcggcgcgga cgccctacca ttcattctca ttacggagag catgtggcaa tactggcggc
     1021 ggttgccttg ctgagtaaag cctttggcgt aattgccgat gcagatggcc tcacgccgct
     1081 ggcaaaaaat cgggcggttt ctgaactgtc aaacgccatc ggcatgcaag gattggttca
     1141 gggtcagttc aaggatctgt ctgaagggga taagccgcgc agcgctgaag ctattttgat
     1201 gacgaatcac tttaaaacca gcacgctgtt ttgtgcctcc atgcagatgg cctcgattgt
     1261 tgcgaatgcc tccagcgaag cgcgtgattg cctgcatcgt ttttcacttg atcttggtca
     1321 ggcatttcaa ctgctggacg atttgaccga tggcatgacc gacaccggta aggatagcaa
     1381 tcaggacgcc ggtaaatcga cgctggtcaa tctgttaggc ccgagggcgg ttgaagaacg
     1441 tctgagacaa catcttcagc ttgccagtga gcatctctct gcggcctgcc aacacgggca
     1501 cgccactcaa cattttattc aggcctggtt tgacaaaaaa ctcgctgccg tcagttaagg
     1561 atgctgcatg agcgcacatg ccctgcccaa ggcagatctg accgccacca gcctgatcgt
     1621 atcgggcggc atcatcgccg cttggctggc cctgcatgtg catgcgctgt ggtttctgga
     1681 cgcagcggcg catcccatcc tggcgatcgc aaatttcctg gggctgacct ggctgtcggt
     1741 cggattgttc atcatcgcgc atgacgcgat gcacgggtcg gtggtgccgg ggcgtccgcg
     1801 cgccaatgcg gcgatgggcc agcttgtcct gtggctgtat gccggatttt cgtggcgcaa
     1861 gatgatcgtc aagcacatgg cccatcaccg ccatgccgga accgacgacg accccgattt
     1921 cgaccatggc ggcccggtcc gctggtacgc ccgcttcatc ggcacctatt tcggctggcg
     1981 cgaggggctg cttctgcccg tcatcgtgac ggtctatgcg ctgatccttg gggatcgctg
     2041 gatgtacgtg gtattctggc cgctgccgtc gatcctggcg tcgatccagc tgttcgtgtt
     2101 cggcacctgg ctgccgcacc gccccggcca cgacgcgttc ccggaccgcc acaatgcgcg
     2161 gtcgtcgcgg atcagcgacc ccgtgtcgct gctgacctgt tttcactttg gcggttatca
     2221 tcacgaacac cacctgcacc cgacggtgcc gtggtggcgc ctgcccagca cccgcaccaa
     2281 gggggacacc gcatgagtga cttaagtggg agcggctatg caaccgcatt atgatctgat
     2341 tctcgtgggg gctggactcg cgaatggcct tatcgccctg cgacttcagc agcagcaacc
     2401 tgatatgcgt attttgctta tcgacgccgc accccaggcg ggcgggaatc atacgtggtc
     2461 atttcaccac gatgatttga ctgagagcca acatcgttgg atagctccgc tggtggttca
     2521 tcactggccc gactatcagg tacgctttcc cacacgccgt cgtaagctga acagcggcta
     2581 cttttgtatt acttctcagc gtttcgctga ggttttacag cgacagtttg gcccgcactt
     2641 gtggatggat accgcggtcg cagaggttaa tgcggaatct gttcggttga aaaagggtca
     2701 ggttatcggt gcccgcgcgg tgattgacgg gcggggttat gcggcaaatt cagcactgag
     2761 cgtgggcttc caggcgttta ttggccagga atggcgattg agccacccgc atggtttatc
     2821 atctcccatt atcatggatg ccacggtcga tcagcaaaat ggttatcgct tcgtgtacag
     2881 cctgccgctc tcgccgacca gattgttaat tgaagatacg cactatattg ataatgcgac
     2941 attagatcct gaatgcgcgc ggcaaaatat ttgcgactat gccgcgcaac agggttggca
     3001 gcttcagaca ctgctgcgag aagaacaggg cgccttaccc attactctgt cgggcaatgc
     3061 cgacgcattc tggcagcagc gccccctggc ctgtagtgga ttacgtgccg gtctgttcca
     3121 tcctaccacc ggctattcac tgccgctggc ggttgccgtg gccgaccgcc tgagtgcact
     3181 tgatgtcttt acgtcggcct caattcacca tgccattacg cattttgccc gcgagcgctg
     3241 gcagcagcag ggctttttcc gcatgctgaa tcgcatgctg tttttagccg gacccgccga
     3301 ttcacgctgg cgggttatgc agcgttttta tggtttacct gaagatttaa ttgcccgttt
     3361 ttatgcggga aaactcacgc tgaccgatcg gctacgtatt ctgagcggca agccgcctgt
     3421 tccggtatta gcagcattgc aagccattat gacgactcat cgttaaagag cgactacatg
     3481 aaaccaacta cggtaattgg tgcaggcttc ggtggcctgg cactggcaat tcgtctacaa
     3541 gctgcgggga tccccgtctt actgcttgaa caacgtgata aacccggcgg tcgggcttat
     3601 gtctacgagg atcaggggtt tacctttgat gcaggcccga cggttatcac cgatcccagt
     3661 gccattgaag aactgtttgc actggcagga aaacagttaa aagagtatgt cgaactgctg
     3721 ccggttacgc cgttttaccg cctgtgttgg gagtcaggga aggtctttaa ttacgataac
     3781 gatcaaaccc ggctcgaagc gcagattcag cagtttaatc cccgcgatgt cgaaggttat
     3841 cgtcagtttc tggactattc acgcgcggtg tttaaagaag gctatctaaa gctcggtact
     3901 gtcccttttt tatcgttcag agacatgctt cgcgccgcac ctcaactggc gaaactgcag
     3961 gcatggagaa gcgtttacag taaggttgcc agttacatcg aagatgaaca tctgcgccag
     4021 gcgttttctt tccactcgct gttggtgggc ggcaatccct tcgccacctc atccatttat
     4081 acgttgatac acgcgctgga gcgtgagtgg ggcgtctggt ttccgcgtgg cggcaccggc
     4141 gcattagttc aggggatgat aaagctgttt caggatctgg gtggcgaagt cgtgttaaac
     4201 gccagagtca gccatatgga aacgacagga aacaagattg aagccgtgca tttagaggac
     4261 ggtcgcaggt tcctgacgca agccgtcgcg tcaaatgcag atgtggttca tacctatcgc
     4321 gacctgttaa gccagcaccc tgccgcggtt aagcagtcca acaaactgca gactaagcgc
     4381 atgagtaact ctctgtttgt gctctatttt ggtttgaatc accatcatga tcagctcgcg
     4441 catcacacgg tttgtttcgg cccgcgttac cgcgagctga ttgacgaaat ttttaatcat
     4501 gatggcctcg cagaggactt ctcactttat ctgcacgcgc cctgtgtcac ggattcgtca
     4561 ctggcgcctg aaggttgcgg cagttactat gtgttggcgc cggtgccgca tttaggcacc
     4621 gcgaacctcg actggacggt tgaggggcca aaactacgcg accgtatttt tgcgtacctt
     4681 gagcagcatt acatgcctgg cttacggagt cagctggtca cgcaccggat gtttacgccg
     4741 tttgattttc gcgaccagct taatgcctat catggctcag ccttttctgt ggagcccgtt
     4801 cttacccaga gcgcctggtt tcggccgcat aaccgcgata aaaccattac taatctctac
     4861 ctggtcggcg caggcacgca tcccggcgca ggcattcctg gcgtcatcgg ctcggcaaaa
     4921 gcgacagcag gtttgatgct ggaggatctg atttgaataa tccgtcgtta ctcaatcatg
     4981 cggtcgaaac gatggcagtt ggctcgaaaa gttttgcgac agcctcaaag ttatttgatg
     5041 caaaaacccg gcgcagcgta ctgatgctct acgcctggtg ccgccattgt gacgatgtta
     5101 ttgacgatca gacgctgggc tttcaggccc ggcagcctgc cttacaaacg cccgaacaac
     5161 gtctgatgca acttgagatg aaaacgcgcc aggcctatgc aggatcgcag atgcacgaac
     5221 cggcgtttgc ggcttttcag gaagtggcta tggctcatga tatcgccccg gcttacgcgt
     5281 ttgatcatct ggaaggcttc gccatggatg tacgcgaagc gcaatacagc caactggatg
     5341 atacgctgcg ctattgctat cacgttgcag gcgttgtcgg cttgatgatg gcgcaaatca
     5401 tgggcgtgcg ggataacgcc acgctggacc gcgcctgtga ccttgggctg gcatttcagt
     5461 tgaccaatat tgctcgcgat attgtggacg atgcgcatgc gggccgctgt tatctgccgg
     5521 caagctggct ggagcatgaa ggtctgaaca aagagaatta tgcggcacct gaaaaccgtc
     5581 aggcgctgag ccgtatcgcc cgtcgtttgg tgcaggaagc agaaccttac tatttgtctg
     5641 ccacagccgg cctggcaggg ttgcccctgc gttccgcctg ggcaatcgct acggcgaagc
     5701 aggtttaccg gaaaataggt gtcaaagttg aacaggccgg tcagcaagcc tgggatcagc
     5761 ggcagtcaac gaccacgccc gaaaaattaa cgctgctgct ggccgcctct ggtcaggccc
     5821 ttacttcccg gatgcgggct catcctcccc gccctgcgca tctctggcag cgcccgctct
     5881 agcgccatgt ctttcccgga gcgtcgcctg aagttttgac aggggcggcg catagaggaa
     5941 gccaaaagaa acacaacctt ctttgcccct gacggcgtga tgcatacgct ggcgattagc
     6001 acgaagacaa gggacttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     6061 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaggca
     6121 atgagacgca cgaagtgatt aattaactgg cctcatgggc cttcacttca ctgcccgctt
     6181 tccagtcggg aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt
     6241 attgggcgct ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc
     6301 ctggggtgcc taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6361 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6421 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6481 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6541 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6601 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6661 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6721 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6781 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6841 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6901 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6961 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7021 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7081 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt attatttacc
     7141 caccactttg gtaatttcgc ctttcacgta atgcacaaat tcttccagct gatccgcacg
     7201 gctggccaga cgatcttctt cctggcccag atacgcctga cgcgcttcca gaatcaccgg
     7261 ctgatactgc gccggcagac gttccatggc ccaatccgcc gccacatctt tcggcgcaat
     7321 tttgccggtc accgcgctat accaaatacg gctcagggtc agcaccacgt tgcgttcatc
     7381 acccgcccaa tccggcgggc tgttccacag ggtcagggtt tcgttcagcg cttcaaacag
     7441 gtcctgttcc ggcaccggat caaacagttc ttccgctgcc ggaccaacca gcgccacgct
     7501 atgttcacgc gctttggtca gcagaatggc cagatcgata tcaatggtcg ccggttcaaa
     7561 aatgccggcc agaatatcgt tacgctgcca ttcgccaaac tgcagctcac gtttcgccgg
     7621 ataacgccac ggaataatgt catcatgcac cacgatggtc acttccaccg cacgcagaat
     7681 ttcgctttca cccgggctcg cgctggtttc cagcagatcg ttaatcagcg cacgacgggt
     7741 ggtttcatcc agacgcacgg tcacggtcac cagcagatca atatcgctat gcggtttcag
     7801 gccgccatcc accgcgctgc catacagatg cacggccagc agggtcggtt ccagatgacg
     7861 ttcaatcacg cccaccactt cagacagctg ggtgctcact tccgcaatca ccgcttcacg
     7921 cataatgttc agtttggttt tcggacggct gccctgctgg gtcacatcgt tgctgctcca
     7981 cagcatcagg caacggccca ccgcataacg cgcctgctgt ttgctcgcac gcggcatgct
     8041 ctgcacgcca aaaaagcaat catagcacgc cataaacacc gccaccgcac cattaccgcc
     8101 gctacgttcg gtcagggtac ggctccaatt acggctacgc atactcttcc tttttcaata
     8161 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8221 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
