LOCUS       sequence-223374-        2925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 module. nptII promoter for constitutive gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG001
FEATURES             Location/Qualifiers
     source          1..2925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(672..691)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(915..932)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1025..1613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1105..1124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(2798..2902)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccacact ctgtggtctc aggagaggcc tgaatcgccc catcatccag
      421 ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat
      481 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc
      541 cttcaactca gcaaaagttc gatttattca acaaagcgac gttgtgtctc aaaatctctg
      601 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
      661 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgagatc
      721 tatcgatgca tgccatggta cccgggagct cgaattcagg cttggaggat acgaatgtga
      781 gaccacgaag tgattaatta actggcctca tgggccttca cttcactgcc cgctttccag
      841 tcgggaaacc tgtcgtgcca gctgcattaa catggtcata gctgtttcct tgcgtattgg
      901 gcgctctccg cttcctcgct cactgactcg ctgcgctcgg tcgttcgggt aaagcctggg
      961 gtgcctaatg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1021 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1441 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1741 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttattat ttacccacca
     1801 ctttggtaat ttcgcctttc acgtaatgca caaattcttc cagctgatcc gcacggctgg
     1861 ccagacgatc ttcttcctgg cccagatacg cctgacgcgc ttccagaatc accggctgat
     1921 actgcgccgg cagacgttcc atggcccaat ccgccgccac atctttcggc gcaattttgc
     1981 cggtcaccgc gctataccaa atacggctca gggtcagcac cacgttgcgt tcatcacccg
     2041 cccaatccgg cgggctgttc cacagggtca gggtttcgtt cagcgcttca aacaggtcct
     2101 gttccggcac cggatcaaac agttcttccg ctgccggacc aaccagcgcc acgctatgtt
     2161 cacgcgcttt ggtcagcaga atggccagat cgatatcaat ggtcgccggt tcaaaaatgc
     2221 cggccagaat atcgttacgc tgccattcgc caaactgcag ctcacgtttc gccggataac
     2281 gccacggaat aatgtcatca tgcaccacga tggtcacttc caccgcacgc agaatttcgc
     2341 tttcacccgg gctcgcgctg gtttccagca gatcgttaat cagcgcacga cgggtggttt
     2401 catccagacg cacggtcacg gtcaccagca gatcaatatc gctatgcggt ttcaggccgc
     2461 catccaccgc gctgccatac agatgcacgg ccagcagggt cggttccaga tgacgttcaa
     2521 tcacgcccac cacttcagac agctgggtgc tcacttccgc aatcaccgct tcacgcataa
     2581 tgttcagttt ggttttcgga cggctgccct gctgggtcac atcgttgctg ctccacagca
     2641 tcaggcaacg gcccaccgca taacgcgcct gctgtttgct cgcacgcggc atgctctgca
     2701 cgccaaaaaa gcaatcatag cacgccataa acaccgccac cgcaccatta ccgccgctac
     2761 gttcggtcag ggtacggctc caattacggc tacgcatact cttccttttt caatattatt
     2821 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     2881 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
