LOCUS       sequence-22338-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  TITLE     Buchler lab plasmids
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p404TEF1
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..405
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     409..425
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     409..425
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(459..475)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(460..476)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(494..512)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      524..713
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(743..761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(771..788)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(771..787)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggagctcata gcttcaaaat gtttctactc cttttttact cttccagatt ttctcggact
       61 ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc atactaaatt tcccctcttt
      121 cttcctctag ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa aaagagaccg
      181 cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt ttctttttct
      241 tgaaaatttt ttttttgatt tttttctctt tcgatgacct cccattgata tttaagttaa
      301 taaacggtct tcaatttctc aagtttcagt ttcatttttc ttgttctatt acaacttttt
      361 ttacttcttg ctcattagaa agaaagcata gcaatctaat ctaagttttc tagaactagt
      421 ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagtca
      481 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa
      541 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
      601 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
      661 catgtaacat tatactgaaa accttgcttg agaaaggttt gggacgctcg aagggcttta
      721 tttgcgggcg gtacccattc ggcctatagt gagtcgtatt acgcgcgctc actggccgtc
      781 gttttacaac gtcgtgact
//
