LOCUS       sequence-223389-        4310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector construction module. pBBR1 medium-copy number origin of 
            replication for construction of Golden Gate cloning vectors .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG011
FEATURES             Location/Qualifiers
     source          1..4310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            525..633
                     /note="incP origin of transfer"
     CDS             complement(644..1306)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      1307..2078
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     primer_bind     complement(2300..2317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2410..2998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2490..2509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(4183..4287)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggccgcatc actctgtgcg tctcaactac ttttccgctg cataaccctg cttcggggtc
      421 attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa
      481 agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa
      541 gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg
      601 ctcaacggga atcctgctct gcgaggctgg ccgtaggccg gccctaccgg cgcggcagcg
      661 ttacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc tcatcgggca
      721 tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag gctggcaggt
      781 ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg ccggtcggta
      841 gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc cccaacagcg
      901 attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac aggcgcaact
      961 ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac acggtgccgg
     1021 ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg actgcgtatt
     1081 ggaccgtccg caaagaacgt ccgatgagct tggaaagagt cttttggctg accaccacgg
     1141 cgttctggtg gcccatctgc gcgacgaggt gatgcagcag cattgccgcc gtgggtttcc
     1201 tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttggacc cagtgaccgg
     1261 gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt atctccatgc
     1321 ggtaggggtg ccgcacggtt gcggcaccat gcgcaatcag ctgcaacttt tcggcagcgc
     1381 gacaacaatt atgcgttgcg taaaagtggc agtcaattac agattttctt taacctacgc
     1441 aatgagctat tgcggggggt gccgcaatga gctgttgcgt accccccttt tttaagttgt
     1501 tgatttttaa gtctttcgca tttcgcccta tatctagttc tttggtgccc aaagaagggc
     1561 acccctgcgg ggttccccca cgccttcggc gcggctcccc ctccggcaaa aagtggcccc
     1621 tccggggctt gttgatcgac tgcgcggcct tcggccttgc ccaaggtggc gctgccccct
     1681 tggaaccccc gcactcgccg ccgtgaggct cggggggcag gcgggcgggc ttcgcccttc
     1741 gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca agccgctgcg
     1801 cggtcgctgc gcgagccttg acccgccttc ctcttggtgt ccaaccggca agcgaagcgc
     1861 gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat ggggcaaggc
     1921 cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag ccggtgggca
     1981 atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc cagcagggtt
     2041 gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg tccacatatc
     2101 cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag caagcccgta
     2161 gggggttact gagacgcacg aagtgctggg cctcatgggc cttcacttca ctgcccgctt
     2221 tccagtcggg aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt
     2281 attgggcgct ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc
     2341 ctggggtgcc taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2401 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2461 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2521 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2581 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2641 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2701 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2761 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2821 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2881 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2941 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatatcaaga
     3001 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3061 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt attatttacc
     3181 caccactttg gtaatttcgc ctttcacgta atgcacaaat tcttccagct gatccgcacg
     3241 gctggccaga cgatcttctt cctggcccag atacgcctga cgcgcttcca gaatcaccgg
     3301 ctgatactgc gccggcagac gttccatggc ccaatccgcc gccacatctt tcggcgcaat
     3361 tttgccggtc accgcgctat accaaatacg gctcagggtc agcaccacgt tgcgttcatc
     3421 acccgcccaa tccggcgggc tgttccacag ggtcagggtt tcgttcagcg cttcaaacag
     3481 gtcctgttcc ggcaccggat caaacagttc ttccgctgcc ggaccaacca gcgccacgct
     3541 atgttcacgc gctttggtca gcagaatggc cagatcgata tcaatggtcg ccggttcaaa
     3601 aatgccggcc agaatatcgt tacgctgcca ttcgccaaac tgcagctcac gtttcgccgg
     3661 ataacgccac ggaataatgt catcatgcac cacgatggtc acttccaccg cacgcagaat
     3721 ttcgctttca cccgggctcg cgctggtttc cagcagatcg ttaatcagcg cacgacgggt
     3781 ggtttcatcc agacgcacgg tcacggtcac cagcagatca atatcgctat gcggtttcag
     3841 gccgccatcc accgcgctgc catacagatg cacggccagc agggtcggtt ccagatgacg
     3901 ttcaatcacg cccaccactt cagacagctg ggtgctcact tccgcaatca ccgcttcacg
     3961 cataatgttc agtttggttt tcggacggct gccctgctgg gtcacatcgt tgctgctcca
     4021 cagcatcagg caacggccca ccgcataacg cgcctgctgt ttgctcgcac gcggcatgct
     4081 ctgcacgcca aaaaagcaat catagcacgc cataaacacc gccaccgcac cattaccgcc
     4141 gctacgttcg gtcagggtac ggctccaatt acggctacgc atactcttcc tttttcaata
     4201 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4261 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
