LOCUS       sequence-223403-        5579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector that targets expression to GABAergic inhibitory neurons 
            in rodent cortex with 98% selectivity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Keaveney MK, Tseng HA, Ta TL, Gritton HJ, Man HY, Han X
  TITLE     A MicroRNA-Based Gene-Targeting Tool for Virally Labeling 
            Interneurons in the Rodent Cortex.
  JOURNAL   Cell Rep. 2018 Jul 10;24(2):294-303. doi: 
            10.1016/j.celrep.2018.06.049.
  PUBMED    29996091
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.06"; journalName: "Cell Rep"; date: 
            "2018-07-10- 10"; volume: "24"; issue: "2"; pages: "294-303"
COMMENT     Sequence Label: AAV-hSyn-EGFP-8X2C (AAV GABA mAGNET)
FEATURES             Location/Qualifiers
     source          1..5579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      71..526
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(158..177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     368..389
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        552..656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(657..725,726..1517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(875..894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1688..2276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2177..2196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2565..2586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2601..2606,2607..2624,2625..2631)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2639..2655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2644..2666
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2663..2679
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2663..2679
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   2693..2822
                     /label=AAV2 ITR
     promoter        2916..3363
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3379..3388
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3385..3387,3388..3390,3391..4104)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3385..3387,3388..3390,3391..4101)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3430..3451)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3691..3710)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4038..4059
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4602..5190
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4655..4675)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5073..5084)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5193..5209)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5194..5210)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5239..5360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5250..5269)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5304..5323
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
       61 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      121 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      181 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      241 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      301 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      361 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      421 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      481 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1681 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1741 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1801 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1861 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1921 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1981 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2041 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2101 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2161 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2221 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2281 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2341 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2401 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgagcga gcgcgcagag
     2461 agggagtggc caactccatc actaggggtt ccttgtagga ttcattaatg cagctggcac
     2521 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     2581 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     2641 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc cactgcgcgc
     2701 tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc
     2761 ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc
     2821 cttgtagtta atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat
     2881 ctgaattctc tagactgcag agggccctgc gtatgagtac aagtgggttt taggaccagg
     2941 atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca
     3001 acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg
     3061 cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc
     3121 ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg
     3181 caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg
     3241 caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc
     3301 gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg
     3361 cagtcgagag gatccgccgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3421 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3481 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatttg caccaccggc
     3541 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3601 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3661 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3721 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3781 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3841 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3901 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3961 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     4021 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4081 ggcatggacg agctgtacaa gtaaactagt gcggccttaa agagaccggt tcactgtgac
     4141 agtaaaagag accggttcac tgtgagaatg aaagagaccg gttcactgtg atcggaaaag
     4201 agaccggttc actgtgagcg gccttaaaga gaccggttca ctgtgacagt aaaagagacc
     4261 ggttcactgt gagaatgaaa gagaccggtt cactgtgatc ggaaaagaga ccggttcact
     4321 gtgagcggcc ttgaaaccca gcagacaatg tagctcagta gaaacccagc agacaatgta
     4381 gctgaatgga aacccagcag acaatgtagc ttcggagaaa cccagcagac aatgtagctg
     4441 cggccttgaa acccagcaga caatgtagct cagtagaaac ccagcagaca atgtagctga
     4501 atggaaaccc agcagacaat gtagcttcgg agaaacccag cagacaatgt agctgcggcc
     4561 gcgactctag agtcgacctg caggcatgca agcttatcga taatcaacct ctggattaca
     4621 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4681 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4741 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4801 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4861 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4921 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4981 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5041 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5101 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5161 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga cccgggcggc
     5221 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     5281 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     5341 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     5401 gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     5461 aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac
     5521 aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactga
//
