LOCUS       sequence-223405-        9645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector (control) that targets expression of EGFP to 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Keaveney MK, Tseng HA, Ta TL, Gritton HJ, Man HY, Han X
  TITLE     A MicroRNA-Based Gene-Targeting Tool for Virally Labeling 
            Interneurons in the Rodent Cortex.
  JOURNAL   Cell Rep. 2018 Jul 10;24(2):294-303. doi: 
            10.1016/j.celrep.2018.06.049.
  PUBMED    29996091
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.06"; journalName: "Cell Rep"; date: 
            "2018-07-10- 10"; volume: "24"; issue: "2"; pages: "294-303"
COMMENT     Sequence Label: pHR-hSyn-EGFP
FEATURES             Location/Qualifiers
     source          1..9645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2255..2702
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2718..2727
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2724..2726,2727..2729,2730..3443)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2724..2726,2727..2729,2730..3440)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2769..2790)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3030..3049)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3377..3398
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3509..4097
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3562..3582)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3980..3991)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4100..4116)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4101..4117)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4207..4440
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4465..4483)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4466..4483)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4773..4792)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5006..5025)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5125..5147
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5185..5203)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5271..5375
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5376..5444,5445..6236)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5594..5613)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6407..6995
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6896..6915
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7149..7166
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7236..7256)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7241..7570
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7421..7556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8704..8769
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8899..8919
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9344..9478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9381..9400)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9435..9454
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattctct agactgcaga gggccctgcg tatgagtgca agtgggtttt aggaccagga
     2281 tgaggcgggg tgggggtgcc tacctgacga ccgaccccga cccactggac aagcacccaa
     2341 cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac aggatgcggc
     2401 gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc ccgcctggcg
     2461 gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc
     2521 aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca gccggaccgc
     2581 accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg
     2641 actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc
     2701 agtcgagagg atccgccgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     2761 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2821 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatttgc accaccggca
     2881 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     2941 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3001 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3061 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3121 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3181 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3241 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3301 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3361 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3421 gcatggacga gctgtacaag taaactagtg cggccgcgac tctagagtcg acctgcaggc
     3481 atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     3541 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3601 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3661 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3721 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3781 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3841 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3901 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     3961 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4021 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4081 ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg agctcggtac
     4141 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4201 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4261 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4321 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4381 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4441 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
     4501 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
     4561 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
     4621 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
     4681 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
     4741 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     4801 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     4861 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     4921 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     4981 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5041 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5101 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5161 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5221 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5281 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5341 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5401 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5461 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5521 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5581 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5641 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5701 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5761 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5821 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5881 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5941 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6001 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6061 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6121 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6181 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6241 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6301 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6361 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6421 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6481 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6541 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6601 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6661 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6721 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6781 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6841 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6901 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6961 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7021 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7081 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7141 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7201 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     7261 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7321 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7381 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     7441 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7501 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7561 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     7621 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     7681 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     7741 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     7801 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     7861 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     7921 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     7981 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     8041 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     8101 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     8161 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     8221 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     8281 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     8341 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     8401 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     8461 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     8521 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     8581 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     8641 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     8701 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     8761 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     8821 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     8881 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     8941 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     9001 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     9061 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     9121 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     9181 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     9241 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     9301 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     9361 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9421 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     9481 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     9541 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     9601 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
