LOCUS       sequence-22341-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  TITLE     Buchler lab plasmids
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p406CYC1
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     322..338
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     322..338
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(372..388)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(373..389)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      391..638
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(407..425)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(656..675)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(657..675)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(685..702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(685..701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(694..716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 accctcacta aagggaacaa aagctggagc tcatttggcg agcgttggtt ggtggatcaa
       61 gcccacgcgt aggcaatcct cgagcagatc cgccaggcgt gtatatatag cgtggatggc
      121 caggcaactt tagtgctgac acatacaggc atatatatat gtgtgcgacg acacatgatc
      181 atatggcatg catgtgctct gtatgtatat aaaactcttg ttttcttctt ttctctaaat
      241 attctttcct tatacattag gacctttgca gcataaatta ctatacttct atagacacgc
      301 aaacacaaat acacacacta atctagaact agtggatccc ccgggctgca ggaattcgat
      361 atcaagctta tcgataccgt cgacctcgag tcatgtaatt agttatgtca cgcttacatt
      421 cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct
      481 aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa
      541 tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc
      601 ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg ccggtaccca attcgcccta
      661 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
      721 ccctggcgtt a
//
