LOCUS       sequence-223413-       11510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology 
            arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression 
            after integration. DR-GFP cassette contains PGK-puromycin. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11510)
  TITLE     CRISPR-mediated insertion of DR-GFP into the AAVS1 locus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAVS1-DRGFP
FEATURES             Location/Qualifiers
     source          1..11510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..61
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     123..142
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             200..220
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             242..262
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      476..485
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(482..484,485..487,488..1201)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSG*G*QGNTYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     1135..1156
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1324..1445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1415..1434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(2106..2705)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2189..2209)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2686..2705
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2724..3221)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2772..2791)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3163..3186
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(3309..3328)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3374..3429
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3428..3447)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     CDS             3793..3813
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3835..3855
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4069..4078
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4075..4077,4078..4080,4081..4539)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(4595..4611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4619..4635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4643..4649,4650..4667,4668..4673))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4688..4709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4814..4949
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    5154..5683
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5830..5850)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5830..5848)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5869..5885)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5893..5909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5917..5923,5924..5941,5942..5947))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5962..5983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6100..6117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6271..6859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6351..6370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7030..7821,7822..7890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7653..7672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7891..7995)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8021..8476)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8158..8179)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8370..8389
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8603..8625
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8617..8634
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8618..8634
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8644..8663
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8644..8662
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8685..9488
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    9495..9520
                     /label=SA
                     /note="splice acceptor site"
     CDS             9544..9597
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9607..10632
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9607..10632
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10664..10681)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10670..10894
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    10901..10982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        11002..11381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
       61 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
      121 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattca gatccgccgc
      181 cactatggga tcaagatcgc caaaaaagaa gagaaaggtg ccgaagaagc atgcagcacc
      241 accaaaaaaa aaacgaaaag tagaagaccc acgaggcaac accagcggcg tgctgagcac
      301 ccccaaggcc aagagggcca agcacccccc cggcaccgag aagcccagga gcaggagcca
      361 gagcgagcag cccgccacct gccccatctg ctacgccgtg atcaggcaga gcaggaacct
      421 gaggaggcac ctggagctga ggcacttcgc caagcccggc gtggatccac cggtcgccac
      481 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      541 cggcgacgta aacggccaca agttcagcgt gtccggctag ggataacagg gtaataccta
      601 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      661 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      721 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      781 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      841 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      901 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      961 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     1021 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1081 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     1141 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     1201 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     1261 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     1321 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1381 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1441 tcttaaggcg taaattgtaa gcgttaatct gaagcttctg atggaattag aacttggcaa
     1501 aacaatactg agaatgaagt gtatgtggaa cagaggctgc tgatctcgtt cttcaggcta
     1561 tgaaactgac acatttggaa accacagtac ttagaaccac aaagtgggaa tcaagagaaa
     1621 aacaatgatc ccacgagaga tctatagatc tatagatcat gagtgggagg aatgagctgg
     1681 cccttaattt ggttttgctt gtttaaatta tgatatccaa ctatgaaaca ttatcataaa
     1741 gcaatagtaa agagccttca gtaaagagca ggcatttatc taatcccacc ccacccccac
     1801 ccccgtagct ccaatccttc cattcaaaat gtaggtactc tgttctcacc cttcttaaca
     1861 aagtatgaca ggaaaaactt ccattttagt ggacatcttt attgtttaat agatcatcaa
     1921 tttcgatccg ctcctgggca ccgaactgcg ccgcgtgttc agcagggtcg gcgtgttcgg
     1981 tgtgtccccc gcggtgggcc tcgggggcgg gtgcggggtc ggcggggccg ccccgggtgg
     2041 cttcggtcgg agccatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc
     2101 gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg
     2161 acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag
     2221 gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg
     2281 gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac
     2341 gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc
     2401 cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg
     2461 acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg
     2521 ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg
     2581 tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg
     2641 gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg
     2701 gtcatggtgg cggctggatc ggtcgaaagg cccggagatg aggaagagga gaacagcgcg
     2761 gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc cggaggacct tcgggcgccc
     2821 gccccgcccc tgagcccgcc cctgagcccg cccccggacc caccccttcc cagcctctga
     2881 gcccagaaag cgaaggagca aagctgctat tggccgctgc cccaaaggcc tacccgcttc
     2941 cattgctcag cggtgctgtc catctgcacg agactagtga gacgtgctac ttccatttgt
     3001 cacgtcctgc acgacgcgag ctgcggggcg ggggggaact tcctgactag gggaggagta
     3061 gaaggtggcg cgaaggggcc accaaagaac ggagccggtt ggcgcctacc ggtggatgtg
     3121 gaatgtgtga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc
     3181 gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagaatt cactcctcag
     3241 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     3301 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     3361 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     3421 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     3481 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     3541 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     3601 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     3661 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     3721 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagctttag
     3781 ggatcaagat cgccaaaaaa gaagagaaag gtgccgaaga agcatgcagc accaccaaaa
     3841 aaaaaacgaa aagtagaaga cccacgaggc aacaccagcg gcgtgctgag cacccccaag
     3901 gccaagaggg ccaagcaccc ccccggcacc gagaagccca ggagcaggag ccagagcgag
     3961 cagcccgcca cctgccccat ctgctacgcc gtgatcaggc agagcaggaa cctgaggagg
     4021 cacctggagc tgaggcactt cgccaagccc ggcgtggatc caccggtcgc caccatggtg
     4081 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4141 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4201 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4261 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     4321 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4381 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4441 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4501 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     4561 aggtgaactt caataaaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     4621 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4681 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4741 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
     4801 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4861 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4921 gaagtagtga ggaggctttt ttggaggcct agtaccatgg aatgcatccc tcgagactag
     4981 ggacaggatt ggtgacagaa aagccccatc cttaggcctc ctccttccta gtctcctgat
     5041 attgggtcta acccccacct cctgttaggc agattcctta tctggtgaca cacccccatt
     5101 tcctggagcc atctctctcc ttgccagaac ctctaaggtt tgcttacgat ggagccagag
     5161 aggatcctgg gagggagagc ttggcagggg gtgggaggga agggggggat gcgtgacctg
     5221 cccggttctc agtggccacc ctgcgctacc ctctcccaga acctgagctg ctctgacgcg
     5281 gctgtctggt gcgtttcact gatcctggtg ctgcagcttc cttacacttc ccaagaggag
     5341 aagcagtttg gaaaaacaaa atcagaataa gttggtcctg agttctaact ttggctcttc
     5401 acctttctag tccccaattt atattgttcc tccgtgcgtc agttttacct gtgagataag
     5461 gccagtagcc agccccgtcc tggcagggct gtggtgagga ggggggtgtc cgtgtggaaa
     5521 actccctttg tgagaatggt gcgtcctagg tgttcaccag gtcgtggccg cctctactcc
     5581 ctttctcttt ctccatcctt ctttccttaa agagtcccca gtgctatctg ggacatattc
     5641 ctccgcccag agcagggtcc cgcttcccta aggccctgct ctgggcttct gggtttgagt
     5701 ccttggcaag cccaggagag gcgctcaggc ttccctgtcc cccttcctcg tccaccatct
     5761 catgcccctg gctctcctgc cccttcccta caggggttcc tggctctgtc tggtacccag
     5821 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     5881 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg ggagcataaa
     5941 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6001 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6061 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6781 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6841 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6901 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6961 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7021 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7081 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7141 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7201 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7261 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7321 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7381 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7441 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7501 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7561 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7621 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7681 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7741 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7801 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7861 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7921 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7981 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     8041 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     8101 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     8161 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     8221 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     8281 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     8341 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     8401 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     8461 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     8521 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     8581 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     8641 gcgtaatacg actcactata gggcgaattg gagctccacc gcggtgcttt ctctgaccag
     8701 cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg ggtcacctct
     8761 acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct ccactccctc
     8821 ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc acttccttct
     8881 cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg tcctctccgg
     8941 gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc ccttttcctt
     9001 ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg acggatgtct
     9061 cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt ctggacaacc
     9121 ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt tctttgcctg
     9181 gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg ggcagctccc
     9241 ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca tggcatcttc
     9301 caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat gtccacttca
     9361 ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc accttatatt
     9421 cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc tccaccccac
     9481 agtggggcag atccctgacc tcttctcttc ctcccacagg gcctcgagag atctggcagc
     9541 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctagg
     9601 ctgcagatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     9661 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     9721 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     9781 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     9841 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     9901 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     9961 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
    10021 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
    10081 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
    10141 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
    10201 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
    10261 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
    10321 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
    10381 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
    10441 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
    10501 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
    10561 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
    10621 gcaaaggaat agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
    10681 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    10741 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    10801 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    10861 agcaggcatg ctggggatgc ggtgggctct atgggtcgag gcagtgaaaa aaatgcttta
    10921 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
    10981 ttaacaacaa caattctggt cgacattgat tattgactag ttattaatag taatcaatta
    11041 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
    11101 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
    11161 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
    11221 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
    11281 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
    11341 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
    11401 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
    11461 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc
//
