LOCUS       sequence-223414-        2657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector construction module. Endlinker/terminator module to 
            circularize plasmid following Part 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG013
FEATURES             Location/Qualifiers
     source          1..2657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(413..499)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(647..664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(757..1345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(837..856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(2530..2634)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggccgcatc aacgagctcc actctgtgcg tctcattact ctagggcggc ggatttgtcc
      421 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact
      481 gagcctttcg ttttatttga tgcctgcctg agacgcacga agtgggtacc ctctgggcct
      541 catgggcctt cacttcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      601 aacatggtca tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg ctcactgact
      661 cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa
      721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1501 aacttggtct gacagttatt atttacccac cactttggta atttcgcctt tcacgtaatg
     1561 cacaaattct tccagctgat ccgcacggct ggccagacga tcttcttcct ggcccagata
     1621 cgcctgacgc gcttccagaa tcaccggctg atactgcgcc ggcagacgtt ccatggccca
     1681 atccgccgcc acatctttcg gcgcaatttt gccggtcacc gcgctatacc aaatacggct
     1741 cagggtcagc accacgttgc gttcatcacc cgcccaatcc ggcgggctgt tccacagggt
     1801 cagggtttcg ttcagcgctt caaacaggtc ctgttccggc accggatcaa acagttcttc
     1861 cgctgccgga ccaaccagcg ccacgctatg ttcacgcgct ttggtcagca gaatggccag
     1921 atcgatatca atggtcgccg gttcaaaaat gccggccaga atatcgttac gctgccattc
     1981 gccaaactgc agctcacgtt tcgccggata acgccacgga ataatgtcat catgcaccac
     2041 gatggtcact tccaccgcac gcagaatttc gctttcaccc gggctcgcgc tggtttccag
     2101 cagatcgtta atcagcgcac gacgggtggt ttcatccaga cgcacggtca cggtcaccag
     2161 cagatcaata tcgctatgcg gtttcaggcc gccatccacc gcgctgccat acagatgcac
     2221 ggccagcagg gtcggttcca gatgacgttc aatcacgccc accacttcag acagctgggt
     2281 gctcacttcc gcaatcaccg cttcacgcat aatgttcagt ttggttttcg gacggctgcc
     2341 ctgctgggtc acatcgttgc tgctccacag catcaggcaa cggcccaccg cataacgcgc
     2401 ctgctgtttg ctcgcacgcg gcatgctctg cacgccaaaa aagcaatcat agcacgccat
     2461 aaacaccgcc accgcaccat taccgccgct acgttcggtc agggtacggc tccaattacg
     2521 gctacgcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2581 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2641 tccccgaaaa gtgccac
//
