LOCUS       sequence-223417-        3339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector construction module. Gentamicin resistance module for 
            construction of Golden Gate cloning vectors .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG009
FEATURES             Location/Qualifiers
     source          1..3339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        join(390..395,396..412,413..418)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             607..1140
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     complement(1329..1346)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1439..2027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1519..1538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(3212..3316)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggccgcatc actctgtgcg tctcagcaat tgacataagc ctgttcggtt cgtaaactgt
      421 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
      481 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagcctatgc
      541 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      601 gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
      661 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
      721 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gcgacgtagc cacctactcc
      781 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac attcatcgcg
      841 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccaag
      901 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg agagcaccgg
      961 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
     1021 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
     1081 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
     1141 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attgactatg
     1201 agacgcacga agtgctgggc ctcatgggcc ttcacttcac tgcccgcttt ccagtcggga
     1261 aacctgtcgt gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc
     1321 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct
     1381 aatgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1441 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1501 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1561 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1621 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1681 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1741 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1801 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1861 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1921 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1981 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atatcaagaa gatcctttga
     2041 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2101 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2161 caatctaaag tatatatgag taaacttggt ctgacagtta ttatttaccc accactttgg
     2221 taatttcgcc tttcacgtaa tgcacaaatt cttccagctg atccgcacgg ctggccagac
     2281 gatcttcttc ctggcccaga tacgcctgac gcgcttccag aatcaccggc tgatactgcg
     2341 ccggcagacg ttccatggcc caatccgccg ccacatcttt cggcgcaatt ttgccggtca
     2401 ccgcgctata ccaaatacgg ctcagggtca gcaccacgtt gcgttcatca cccgcccaat
     2461 ccggcgggct gttccacagg gtcagggttt cgttcagcgc ttcaaacagg tcctgttccg
     2521 gcaccggatc aaacagttct tccgctgccg gaccaaccag cgccacgcta tgttcacgcg
     2581 ctttggtcag cagaatggcc agatcgatat caatggtcgc cggttcaaaa atgccggcca
     2641 gaatatcgtt acgctgccat tcgccaaact gcagctcacg tttcgccgga taacgccacg
     2701 gaataatgtc atcatgcacc acgatggtca cttccaccgc acgcagaatt tcgctttcac
     2761 ccgggctcgc gctggtttcc agcagatcgt taatcagcgc acgacgggtg gtttcatcca
     2821 gacgcacggt cacggtcacc agcagatcaa tatcgctatg cggtttcagg ccgccatcca
     2881 ccgcgctgcc atacagatgc acggccagca gggtcggttc cagatgacgt tcaatcacgc
     2941 ccaccacttc agacagctgg gtgctcactt ccgcaatcac cgcttcacgc ataatgttca
     3001 gtttggtttt cggacggctg ccctgctggg tcacatcgtt gctgctccac agcatcaggc
     3061 aacggcccac cgcataacgc gcctgctgtt tgctcgcacg cggcatgctc tgcacgccaa
     3121 aaaagcaatc atagcacgcc ataaacaccg ccaccgcacc attaccgccg ctacgttcgg
     3181 tcagggtacg gctccaatta cggctacgca tactcttcct ttttcaatat tattgaagca
     3241 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3301 aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
