LOCUS       sequence-223423-        4064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 module. Taurine-inducible promoter for Alphaproteobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG041
FEATURES             Location/Qualifiers
     source          1..4064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             159..950
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1121..1709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1610..1629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1863..1880
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3811..3829)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3897..4001
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct cgggcatcca
       61 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
      121 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg
      181 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
      241 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagcgata
      301 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
      361 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
      421 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
      481 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
      541 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
      601 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
      661 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
      721 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
      781 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
      841 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
      901 aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc
      961 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1021 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1081 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1141 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1201 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1261 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1321 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1381 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1441 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1501 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1561 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1621 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1681 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1741 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1801 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1861 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     1921 cgcgttggcc gattcattaa tcactctgtg gtctcaggag ggctttacta agctgatccg
     1981 gtggatgacc ttttgaatga cctttaatag attatattac taattaattg gggaccctag
     2041 aggtcccctt ttttatttta aaaatttttt cacaaaacgg tttacaagca taaagcttgc
     2101 tcaatcaatc accggatcta gatatcgata ccccgctccg cgatccgcgc cgcagtcagg
     2161 gccacgccgt cggctatgcg gttgcgcgcg atcgacgagt agcccatgcg gaagaagcgg
     2221 catgggccgt cgtccttcgg gaagaagggc gacccggatt cgatcagcac gccgtccttt
     2281 cgcaaagcgt tcatcagcaa gtccgcatcc agaccttccg gcccctcgat ccagaaggac
     2341 gtgccgccga aagccgcaga tccggcgatc tccaatccgg catcccggag ggcagcgtcc
     2401 atgatgatgt ggcggcgatg atactcctcg cgcatgcggt gcagcacggc atcatagtgt
     2461 ccgagcgcca ggaaataggc ggccgttctc tggaggtgcc ccggcggatg ccgcaacatc
     2521 aaggcccgta gtgcccgcgc ctcccggatc acggcagccg gcgccaccag atagcccaga
     2581 cgcaggccgg gaaacaacga cttggagaaa ctgccgatgt agaacacccg gccgcagcgg
     2641 tcgaaggcct tgagggccgg ggaggggggc gccagaaagc tcatctcgaa ctcgtaatcg
     2701 tcctcgacga ttatgaagtc cttggaggcg gccgcctcga gcaaggccgc ccgccgttcg
     2761 atcggcatcg tcgcgccggt cggcgaatga tggctgggcg tgacgaacac ggcatcgacc
     2821 tcttctggaa gcgcggcggg cggcaggccg tccttgtcca cgtcgacgaa ggtgatcccg
     2881 gcgccgctca agcgaagcga tgcactcatg tcgggatggc aggggttctc gcagaccgca
     2941 tgggcgccct gacgcaacag gagctggatg acgatccaga gggcgttctg cgcgcccacc
     3001 gtcaccagga tctcgtcggg gttcgcgcgg atgccgcggc ccggcaatgt gcgcgagcag
     3061 atatagttga caagctggat gtcgtccgcc gccgcgaagt cgcccgccat cagctcgaaa
     3121 tcctcgcgcg ccagcgcccg tcgcgcgcag tcgcgccagg ccgtgaggtc gaacagcgac
     3181 gggtccatct gaccgtatag gaacggataa gggtaggttc gccagtcgag gggcttacgc
     3241 atctgcttgg ccacgatgaa ggtcgagcgc aacttggccg accagtcgat ggcatcggcc
     3301 gcagcatggg cggcctgcat cgcgatggct gtcgttgggg gcttgccggc cacacggtag
     3361 ccgctccggt tcgccgcctc cacatagccc tgggaagcga gttcctgata ggcgagcgtt
     3421 acggtgatcc gcgaaatatt cagataggcc gcaagcttgc ggctggaggg caattgggcg
     3481 cccggcgcca ggcggcccga caatacggcc gaaacgatcg tttcgcggat ctgcgcctgc
     3541 aggcccgttt ggctggcccg gtcaaggaaa aagatcgttt cggacacggt cgagcccgcc
     3601 attgttcacc tcgcccaact ggactcatct aacttcctat ctggatataa ggcaatccga
     3661 agcgtgggcc tatttctatt cagcaagcaa agaccgtcat tcaatgtacg gcgcgacccc
     3721 gcaaaaagcg gggcgacata accaaagagg gtaacgacag gaggaagaac aaatgtgaga
     3781 ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta
     3841 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     3901 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3961 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt
     4021 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcg
//
