LOCUS       sequence-223425-        4211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector construction module. Tetracycline resistance module for 
            construction of Golden Gate cloning vectors .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG042
FEATURES             Location/Qualifiers
     source          1..4211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(127..1326)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             1432..2082
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(2164..2181)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2335..2923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2415..2434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3094..3885)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(4107..4211)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtccactg aagagccact tcgtgcgtct
      121 catagttcag cgatcggctc gttgccctgc gccgctccaa agcccgcgac gcagcgccgg
      181 caggcagagc aagtagaggg cagcgcctgc aatccatgcc cacccgttcc acgttgttat
      241 agaagccgca tagatcgccg tgaagaggag gggtccgacg atcgaggtca ggctggtgag
      301 cgccgccagt gagccttgca gctgcccctg acgttcctca tccacctgcc tggacaacat
      361 tgcttgcagc gccggcattc cgatgccacc cgaagcaagc aggaccatga tcgggaacgc
      421 catccatccc cgtgtcgcga aggcaagcag gatgtagcct gtgccgtcgg caatcattcc
      481 gagcatgagt gcccgccttt cgccgagccg ggcggctaca gggccggtga tcattgcctg
      541 ggcgagtgaa tgcagaatgc caaatgcggc aagcgaaatg ccgatcgtgg tcgcgtccca
      601 gtgaaagcga tcctcgccga aaatgaccca aagcgcggcc ggcacctgtc cgacaagttg
      661 catgatgaag aacaccgcca tcagggcggc gacgacggtc atgccccggg cccaccggaa
      721 cgaagcgagc gggttgagag cctcccggcg taacggccgg cgttcgcctt tgtgcgactc
      781 cggcaaaagg aaacagcccg tcaggaaatt gaggccgttc aaggctgccg cggcgaagaa
      841 cggagcgtgg ggggagaaac cgcccatcag cccaccgagc acaggtcccg cgaccatccc
      901 gaacccgaaa caggcgctca tgaagccgaa gtgccgcgcg cgctcatcgc catcagtgat
      961 atcggcaata taagcgccgg ctaccgcccc agtcgccccg gtgatgccgg ccacgatccg
     1021 cccgatatag agaacccaaa ggaaaggcgc tgtcgccatg atggcgtagt cgacagtggc
     1081 gccggccagc gacacgagca agattggccg ccgcccgaaa cgatccgaca gcgcgcccag
     1141 cacaggtgcg caggcaaatt gcaccaacgc atacagcgcc agcagaatgc catagtgggc
     1201 ggtgacgtcg ttcgagtgaa ccagatcgcg caggaggccc ggcagcaccg gcataatcag
     1261 gccgatgccg acagcgtcga gcgcgacagt gctcagaatt acgatcaggg gtatgttggg
     1321 tttcacgtct ggcctccgga ccagcctccg ctggtccgat tgaacgcgcg gattctttat
     1381 cactgataag ttggtggaca tattatgttt atcagtgata aagtgtcaag catgacaaag
     1441 ttgcagccga atacagtgat ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac
     1501 ggtctgacga cacgcaaact ggcggaacgg ttgggggttc agcagccggc gctttactgg
     1561 cacttcagga acaagcgggc gctgctcgac gcactggccg aagccatgct ggcggagaat
     1621 catacgcatt cggtgccgag agccgacgac gactggcgct catttctgat cgggaatgcc
     1681 cgcagcttca ggcaggcgct gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg
     1741 cgaccgggcg caccgcagat ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg
     1801 ggtttttcgg ccggggacgc cgtcaatgcg ctgatgacaa tcagctactt cactgttggg
     1861 gccgtgcttg aggagcaggc cggcgacagc gatgccggcg agcgcggcgg caccgttgaa
     1921 caggctccgc tctcgccgct gttgcgggcc gcgatagacg ccttcgacga agccggtccg
     1981 gacgcagcgt tcgagcaggg actcgcggtg attgtcgatg gattggcgaa aaggaggctc
     2041 gttgtcagga acgttgaagg accgagaaag ggtgacgatt gattgctgag acgcacagag
     2101 tgattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2161 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2221 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2281 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2341 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2401 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2461 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2521 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2581 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2641 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2701 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2761 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2821 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2881 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2941 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3001 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3061 ctaaagtata tatgagtaaa cttggtctga cagttatttg ccgactacct tggtgatctc
     3121 gccttttacg taatggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     3181 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     3241 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     3301 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     3361 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     3421 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     3481 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     3541 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     3601 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     3661 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     3721 ggtcaccgta accagcaaat caatatcgct gtgtggcttc aggccgccat ccactgcgga
     3781 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     3841 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt
     3901 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     3961 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaaa
     4021 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     4081 gttctggacc agttgcgtga gcgcatactc ttcctttttc aatattattg aagcatttat
     4141 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4201 ggggttccgc g
//
