LOCUS       sequence-223435-        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of PKIa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Billiard J, Grewal SS, Lukaesko L, Stork PJ, Rotwein P
  TITLE     Hormonal control of insulin-like growth factor I gene transcription 
            in human osteoblasts: dual actions of cAMP-dependent protein kinase 
            on CCAAT/enhancer-binding protein delta.
  JOURNAL   J Biol Chem. 2001 Aug 17;276(33):31238-46. doi: 
            10.1074/jbc.M103634200. Epub 2001 Jun 4.
  PUBMED    11390399
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M103634200"; journalName: "J Biol Chem"; date: 
            "2001-08-17- 17"; volume: "276"; issue: "33"; pages: "31238-46"
COMMENT     Sequence Label: GFP-PKI nls
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(64..66,67..69,70..780)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(64..66,67..69,70..780)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(109..130)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(370..389)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     717..738
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             805..852
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             967..987
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1009..1029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgggggctat tagcagagct ggtttagtga ccgtcagatc cgctagcgct accggtcgcc
       61 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      121 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      181 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      241 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      301 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      361 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      421 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      481 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
      541 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      601 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      661 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
      721 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      781 tccggactca gatctcgaga gatggactac aaggacgacg atgacaagga ctacaaggac
      841 gacgatgaca agaattctac tgatgtggaa actacgtatg cagattttat tgcctcagga
      901 agaacaggta gaagaaatgc aatacatgat atcttggttt cctctgcaag tggcaacagc
      961 aatgaaccta agaagaaacg caaggtcggt accggaggag gtgaagcacc caagaaaaag
     1021 cgaaaagtag gatccaccgg atctagatac tgatcatatc agcatacaca tttgtagagt
     1081 ttactgcttt aaaactccca cactccccct gacctgaacc ataaatgatg cattggtgtg
     1141 ttacttgata atgccagctt ataatggctt aca
//
