LOCUS       sequence-223452-        1238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of bovine Rap1b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Liu C, Takahashi M, Li Y, Dillon TJ, Kaech S, Stork PJ
  TITLE     The interaction of Epac1 and Ran promotes Rap1 activation at the 
            nuclear envelope.
  JOURNAL   Mol Cell Biol. 2010 Aug;30(16):3956-69. doi: 10.1128/MCB.00242-10. 
            Epub 2010 Jun 14.
  PUBMED    20547757
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00242-10"; journalName: "Mol Cell Biol"; date: 
            "2010-08"; volume: "30"; issue: "16"; pages: "3956-69"
COMMENT     Sequence Label: Flag-Rap1b N17
FEATURES             Location/Qualifiers
     source          1..1238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..79
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     promoter        complement(653..671)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(654..671)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(691..708)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    697..918
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcgaagggtc gagctcggat ccgccaccat ggactacaag gacgacgatg acaaggacta
       61 caaggacgac gatgacaaga attctcgtga gtataagcta gtcgttcttg gctctggagg
      121 cgttggaaaa aatgctctga ctgtacagtt tgttcaagga atttttgttg aaaaatatga
      181 tcctacgata gaagattctt atagaaagca agttgaagta gatgcacagc agtgtatgct
      241 tgaaatcttg gatactgcag gaacggaaca attcacagca atgagggatt tgtacatgaa
      301 aaatggacaa ggctttgcgt tagtttattc catcacagca cagtccacat ttaatgattt
      361 acaagatctg agagaacaga tacttcgagt taaagacacg gatgatgttc caatgattct
      421 ggttggtaat aagtgtgact tggaagatga aagagttgta ggaagggaac aaggtcaaaa
      481 tctagcaaga cagtggaaca actgtgcatt cttagaatcc tctgcaaaat caaaaataaa
      541 tgttaatgag atcttttatg acctagtgcg acaaattaac agaaaaactc cagtgcctgg
      601 gaaggcccgc aaaaagtcat catgtcagct gctttaatct agagggccct attctatagt
      661 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
      721 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      781 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
      841 ggggtggggt ggggcagaca gcaaggggga ggattggaag acaatagcag gcatgctggg
      901 gatgcgtggg ctctatggct tctgagcgga aagaacagct ggggctctag gggtatcccc
      961 acgcgcctgt agcgccgcat taagcgcgcg gtgtggtggt taccgcgcag cgtgaccgct
     1021 acacttgcca gcgcctagcg ccgctctttc gctttcttcc atcgtttctc gccaggttcg
     1081 cagctttccc ggtcaagctc tagtcgggct cctttaggtc gatttatgct taacgacttc
     1141 acccaaaact tgatagggga atgttcacgt atggcatccc ctgatgacgt tgccttgacg
     1201 tgaatcgatc ctatgtggaa actgcttgta tcacggac
//
