LOCUS       sequence-223456-        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of bovine Rap1b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Takahashi M, Dillon TJ, Liu C, Kariya Y, Wang Z, Stork PJ
  TITLE     Protein kinase A-dependent phosphorylation of Rap1 regulates its 
            membrane localization and cell migration.
  JOURNAL   J Biol Chem. 2013 Sep 27;288(39):27712-23. doi: 
            10.1074/jbc.M113.466904. Epub 2013 Aug 14.
  PUBMED    23946483
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.466904"; journalName: "J Biol Chem"; date: 
            "2013-09-27- 27"; volume: "288"; issue: "39"; pages: "27712-23"
COMMENT     Sequence Label: Flag-Rap1b
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..75
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     promoter        complement(649..667)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(650..667)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(687..704)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    693..918
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cagctcgagc tcggatccgc caccatggac tacaaggacg acgatgacaa ggactacaag
       61 gacgacgatg acaagaattc tcgtgagtat aagctagtcg ttcttggctc tggaggcgtt
      121 ggaaaatctg ctctgactgt acagtttgtt caaggaattt ttgttgaaaa atatgatcct
      181 acgatagaag attcttatag aaagcaagtt gaagtagatg cacagcagtg tatgcttgaa
      241 atcttggata ctgcaggaac ggaacaattc acagcaatga gggatttgta catgaaaaat
      301 ggacaaggct ttgcgttagt ttattccatc acagcacagt ccacatttaa tgatttacaa
      361 gatctgagag aacagatact tcgagttaaa gacacggatg atgttccaat gattctggtt
      421 ggtaataagt gtgacttgga agatgaaaga gttgtaggaa aggaacaagg tcaaaatcta
      481 gcaagacagt ggaacaactg tgcattctta gaatcctctg caaaatcaaa aataaatgtt
      541 aatgagatct tttatgacct agtgcgacag attaacagaa aaactccagt gcctgggaag
      601 gcccgcaaaa agtcgtcatg tcagctgctt taatctagag ggccctattc tatagtgtca
      661 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
      721 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
      781 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
      841 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcacg catgctgggg
      901 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
      961 cccacgcgcc ctgtagcggc gcattaagcg cgcggtgtgt gtacgcgcag cgtgaccgct
     1021 acactgcagc gcctagcgcc ccgctccttc gctttcttcc atcgttctcg ccacgttcgc
     1081 tgctttccgg tcaggctcta atccgagttc cgttaagttc cgaattagct acgacctgaa
     1141 ccaaaacctg t
//
