LOCUS       sequence-223463-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human Craf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Takahashi M, Li Y, Dillon TJ, Kariya Y, Stork PJS
  TITLE     Phosphorylation of the C-Raf N-region promotes Raf dimerization.
  JOURNAL   Mol Cell Biol. 2017 Jul 10. pii: MCB.00132-17. doi: 
            10.1128/MCB.00132-17.
  PUBMED    28694330
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2017 Jul 10. pii: MCB.00132-17. doi: 10.1128/MCB.00132-17."
COMMENT     Sequence Label: Flag-Craf R89L
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..75
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 attgtagagc tcggatccgc caccatggac tacaaggacg acgatgacaa ggactacaag
       61 gacgacgatg acaagaattc tcgagtaacc atggagcaca tacagggagc ttggaagacg
      121 atcagcaatg gttttggatt caaagatgcc gtgtttgatg gctccagctg catctctcct
      181 acaatagttc agcagtttgg ctatcagcgc cgggcatcag atgatggcaa actcacagat
      241 ccttctaaga caagcaacac tatccgtgtt ttcttgccga acaagcaaag aacagtggtc
      301 aatgtgcgaa atggaatgag cttgcatgac tgccttatga aagcactcaa ggtgctgggc
      361 ctgcaaccag agtgctgtgc agtgttcaga cttctccacg aacacaaagg taaaaaagca
      421 cgcttagatt ggaatactga tgctgcgtct ttgattggag aagaacttca agtagatttc
      481 ctggatcatg ttcccctcac aacacacaac tttgctcgga agacgttcct gaagcttgcc
      541 ttctgtgaca tctgtcagaa attcctgctc aatggatttc gatgtcagac ttgtggctac
      601 aaatttcatg agcactgtag caccaaagta cctactatgt gtgtggactg gagtaacatc
      661 agacaactct tattgtttcc aaattccact attggtgata gtggagtccc agcact
//
