LOCUS       sequence-223472-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PTet*-YFP Reporter.  aTc sensor for use in Marionette strain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA
  TITLE     Escherichia coli ""Marionette"" strains with 12 highly optimized 
            small-molecule sensors.
  JOURNAL   Nat Chem Biol. 2018 Nov 26. pii: 10.1038/s41589-018-0168-3. doi: 
            10.1038/s41589-018-0168-3.
  PUBMED    30478458
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Nov 26. pii: 10.1038/s41589-018-0168-3. doi: 
            10.1038/s41589-018-0168-3."
COMMENT     Sequence Label: pAJM.717
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(499..518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcatggacg agctgtacaa gtaactcggt accaaattcc agaaaagagg cctcccgaaa
       61 ggggggcctt ttttcgtttt ggtccaatgg cggcgcgcca tcgaatgagg tgcttcctcg
      121 ctcactgact cgctgcacga ggcagacctc agcgctagcg gagtgtatac tggcttacta
      181 tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca
      241 ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg
      301 ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc
      361 ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt
      421 tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc
      481 gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct
      541 ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
      601 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
      661 gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      721 ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt
      781 agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc
      841 tcctccaagc cagttacctc
//
