LOCUS       sequence-22348-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Dickerson SK, Market E, Besmer E, Papavasiliou FN
  TITLE     AID mediates hypermutation by deaminating single stranded DNA.
  JOURNAL   J Exp Med. 2003 May 19. 197(10):1291-6.
  PUBMED    12756266
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2003 May 19. 197(10):1291-6."
COMMENT     Sequence Label: pET3d::strep-AID
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..62
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             75..668
                     /codon_start=1
                     /gene="AICDA"
                     /product="human activation-induced cytidine deaminase 
                     (Dickerson et al., 2003)"
                     /label=AID
                     /translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
                     FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPYL
                     SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
                     GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
     primer_bind     complement(728..746)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tggtggcctg gagccacccg cagttcgaaa
       61 aaggcggcgg atcgatggac agcctcttga tgaaccggag gaagtttctt taccaattca
      121 aaaatgtccg ctgggctaag ggtcggcgtg agacctacct gtgctacgta gtgaagaggc
      181 gtgacagtgc tacatccttt tcactggact ttggttatct tcgcaataag aacggctgcc
      241 acgtggaatt gctcttcctc cgctacatct cggactggga cctagaccct ggccgctgct
      301 accgcgtcac ctggttcacc tcctggagcc cctgctacga ctgtgcccga catgtggccg
      361 actttctgcg agggaacccc tacctcagtc tgaggatctt caccgcgcgc ctctacttct
      421 gtgaggaccg caaggctgag cccgaggggc tgcggcggct gcaccgcgcc ggggtgcaaa
      481 tagccatcat gaccttcaaa gattattttt actgctggaa tacttttgta gaaaaccatg
      541 aaagaacttt caaagcctgg gaagggctgc atgaaaattc agttcgtctc tccagacagc
      601 ttcggcgcat ccttttgccc ctgtatgagg ttgatgactt acgagacgca tttcgtactt
      661 tgggacttct cgactaagga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      721 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      781 ggttt
//
