LOCUS       sequence-223482-        1425 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the His tagged Saccharomyces cerevisiae Ornithine 
            Decarboxylase in Escherichia coli BL21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Olin-Sandoval V, Yu JSL, Miller-Fleming L, Alam MT, Kamrad S, 
            Correia-Melo C, Haas R, Segal J, Pena Navarro DA, Herrera-Dominguez 
            L, Mendez-Lucio O, Vowinckel J, Mulleder M, Ralser M
  TITLE     Lysine harvesting is an antioxidant strategy and triggers 
            underground polyamine metabolism.
  JOURNAL   Nature. 2019 Aug;572(7768):249-253. doi: 10.1038/s41586-019-1442-6. 
            Epub 2019 Jul 31.
  PUBMED    31367038
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1442-6"; journalName: "Nature"; date: "2019-08";
            volume: "572"; issue: "7768"; pages: "249-253"
COMMENT     Sequence Label: ScSpe1 pET-20b(+)
FEATURES             Location/Qualifiers
     source          1..1425
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1405..1422
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtctagta ctcaagtagg aaatgctcta tctagttcca ctactacttt agtggacttg
       61 tctaattcta cggttaccca aaagaagcaa tattataaag atggcgagac gctgcacaat
      121 cttttgcttg aactaaagaa taaccaagat ttggaacttt taccgcatga acaagcgcat
      181 cctaaaatat ttcaagcgct caaggctcgt attggtagaa ttaataatga aacgtgcgac
      241 cccggtgagg agaactcgtt tttcatatgc gatttgggag aagtcaagag attattcaac
      301 aactgggtga aggagcttcc tagaattaag ccattttatg ccgtcaaatg taatcctgat
      361 accaaggttt tgtcattatt agcagagttg ggcgttaatt tcgattgcgc ttccaaagtg
      421 gaaattgaca gagtattatc gatgaacatc tcgccggata gaattgttta cgctaatcct
      481 tgtaaagtag catctttcat tagatatgca gcttcaaaaa atgtaatgaa gtctactttt
      541 gacaatgtag aagaattgca taaaatcaaa aagtttcatc ctgagtctca gttgttatta
      601 agaatcgcta ccgatgactc taccgctcaa tgtcgacttt ccaccaaata tggctgtgaa
      661 atggaaaacg tagacgtttt attaaaggct ataaaggaac taggtttaaa cctggctggt
      721 gtttctttcc acgtcggttc aggcgcttct gattttacaa gcttatacaa agccgttaga
      781 gatgcaagaa cggtatttga caaagctgct aacgaatacg ggttgccccc tttgaagatt
      841 ttggatgtag gtggtggatt tcaatttgaa tccttcaaag aatcaactgc tgttttgcgt
      901 ctagcgctag aggaattttt ccctgtaggt tgtggtgttg atataattgc agagcctggc
      961 agatactttg tagctacagc gttcactttg gcatctcatg tgattgcgaa gagaaaactg
     1021 tctgagaatg aagcaatgat ttacactaac gatggtgtat acgggaacat gaattgtatt
     1081 ttattcgatc atcaagagcc ccatccaaga accctttatc ataatttgga atttcattac
     1141 gacgattttg aatccactac tgcggtcctc gactctatca acaaaacaag atctgagtat
     1201 ccatataaag tttccatctg gggacccaca tgtgatggtt tggattgtat tgccaaagag
     1261 tattacatga agcatgatgt tatagtcggt gattggtttt attttcctgc cctgggtgcc
     1321 tacacatcat cggcggctac tcaattcaac ggctttgagc agactgcgga tatagtatac
     1381 atagactctg aactcgatct cgagcaccac caccaccacc actga
//
