LOCUS       sequence-22350-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Li X, Monks B, Ge Q, Birnbaum MJ
  TITLE     Akt/PKB regulates hepatic metabolism by directly inhibiting 
            PGC-1alpha transcription coactivator.
  JOURNAL   Nature. 2007 Jun 21. 447(7147):1012-6.
  PUBMED    17554339
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 21. 447(7147):1012-6."
COMMENT     Sequence Label: pcDNA Flag PGC1alpha S570A
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             160..183
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagttaag cttggtaccg agctcggatc cactagtcca
      121 gtgtggtgga attctgcaga tatccggatc accaccatgg actacaaaga cgatgacgat
      181 aaagcaagaa gagcatctgt gggatcctgc agccaagact ctgtatggag tgacatagag
      241 tgtgctgctc tggttggtga ggaccagcct ctttgcccag atcttcctga acttgacctt
      301 tctgaacttg atgtgaatga cttggataca gacagctttc tgggtggatt gaagtggtgt
      361 agcgaccaat cggaaatcat atccaaccag tacaacaatg agcctgcgaa catatttgag
      421 aagatagatg aagagaatga ggcaaacttg ctagcggtcc tcacagagac actggacagt
      481 ctccccgtgg atgaagacgg attgccctca tttgatgcac tgacagatgg agccgtgacc
      541 actgacaacg aggccagtcc ttcctccatg cctgacggca cccctccccc tcaggaggca
      601 gaagagccgt ctctacttaa gaagctctta ctggcaccag ccaacactca gctcagctac
      661 aatgaatgca gcggtcttag cactcagaac catgcagcaa accacaccca caggatcaga
      721 acaaaccctg ccattgttaa gaccgagaat tcatggagca ataaagcgaa gagcattttg
      781 tcaca
//
