LOCUS       sequence-223501-        9674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  U6 driven Spy sgRNA cloning vector where guide sequences are 
            inserted between BspMI sites. This plasmid works with Addgene 
            #108570 for subnuclear proteomic profiling via C-BERST method..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Gao XD, Rodriguez TC, Sontheimer EJ
  TITLE     Adapting dCas9-APEX2 for subnuclear proteomic profiling.
  JOURNAL   Methods Enzymol. 2019;616:365-383. doi: 10.1016/bs.mie.2018.10.030. 
            Epub 2018 Dec 10.
  PUBMED    30691651
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2019"; volume: "616:365-383. doi"; pages: " 
            10.1016/bs.mie.2018.10.030. Epub 2018 Dec 10"
COMMENT     Sequence Label: pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA
FEATURES             Location/Qualifiers
     source          1..9674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     426..444
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          516..1088
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1096..1115
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1143..1161
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1172..1792
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1245..1264)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             1826..1882
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1898..2608
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGIYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             2609..2629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             2725..2958
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3030..3151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3067..3086)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3121..3140
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3191..3326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3347..3365)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3375..3392)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3375..3391)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3384..3406)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3533..3988
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3620..3639)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3830..3851
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4014..4118
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4119..4187,4188..4979)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4337..4356)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5150..5738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5639..5658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5892..5909
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6026..6047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6062..6067,6068..6085,6086..6092)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6100..6116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6105..6127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6124..6140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6124..6140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6159..6179
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6161..6179
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6207..6433
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6434..6614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6661..6786
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7279..7512
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7697..7741
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8039..8287
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8039..8059
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8210..8229
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     CDS             complement(8418..9212)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8529..8548)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9139..9158
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    9505..9622
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9671..9674,1..507)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatccgtgag tttggggacc cttgattgtt
      541 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
      601 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
      661 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
      721 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
      781 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
      841 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
      901 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc
      961 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     1021 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt
     1081 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     1141 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat
     1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag
     1801 cggctcccgg gagttcgcta gcggtgctac taacttcagc ctgctgaagc aggctggaga
     1861 cgtggaggag aaccctggac ctggtagtgg aacgcgtatg gtgtctaagg gcgaagagct
     1921 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     1981 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     2041 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     2101 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     2161 ccctgagggc ttcacatggg agagagtcac cacatacgaa gatgggggcg tgctgaccgc
     2221 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     2281 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     2341 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     2401 cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca agaaacccgc
     2461 taagaacctc aagatgcctg gcatctacta tgtggactac agactggaaa gaatcaagga
     2521 ggccaacaac gaaacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     2581 ccctagcaaa ctggggcaca agcttaatcc aaagaaaaag cggaaagtgt gagtcgacct
     2641 cgaggggggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     2701 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agaaaagatc
     2761 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     2821 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     2881 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     2941 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     3001 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3061 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3121 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     3181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3241 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3301 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     3361 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3421 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3481 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3541 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3601 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3661 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3721 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3781 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3841 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3901 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3961 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4081 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4141 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4201 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4261 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4321 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4381 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4441 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4501 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4561 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4621 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4681 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4741 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4801 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4861 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4921 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4981 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5041 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5281 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5941 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6001 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6061 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6121 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6181 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6241 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6301 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6361 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6421 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6481 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6541 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6601 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6661 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     6721 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     6781 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     6841 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     6901 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     6961 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7021 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7081 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7141 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7201 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7261 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7321 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7381 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7441 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7501 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7561 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7621 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7681 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7741 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7801 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7861 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     7921 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     7981 gaacggatct cgacggtatc gatcacgaga ctagcctcga gcggccgccc ccttcaccga
     8041 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     8101 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     8161 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     8221 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     8281 aaacaccggt gtgcaggtga gtgatccaaa cgcccggcgg caaccgagcg ttctgaacaa
     8341 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctctc
     8401 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     8461 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     8521 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     8581 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     8641 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
     8701 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     8761 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     8821 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     8881 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     8941 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     9001 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
     9061 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     9121 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     9181 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     9241 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     9301 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga cggatcaaga
     9361 acctgctgac gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc
     9421 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttgaattct cgacctcgag
     9481 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     9541 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9601 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
     9661 gcggctcgag gggg
//
