LOCUS       sequence-22351-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Tuttle RL, Gill NS, Pugh W, Lee JP, Koeberlein B, Furth EE, Polonsky
            KS, Naji A, Birnbaum MJ
  TITLE     Regulation of pancreatic beta-cell growth and survival by the 
            serine/threonine protein kinase Akt1/PKBalpha.
  JOURNAL   Nat Med. 2001 Oct . 7(10):1133-7.
  PUBMED    11590437
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2001 Oct . 7(10):1133-7."
COMMENT     Sequence Label: pLNCX1 myr Akt1 delta4-129
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             133..174
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg cggccccaag cttgtcgacg gtaccccggg
      121 gaattcgccg ccatggggag tagcaagagc aagcctaagg accccagcca gcgcagcgag
      181 gctgaagaga tggaggtgtc cctggccaag cccaagcacc gcgtgaccat gaacgagttt
      241 gagtacctga agctgctggg caagggcact ttcggcaagg tgatcctggt gaaggagaag
      301 gccacaggcc gctactacgc catgaagatc ctcaagaagg aagtcatcgt ggccaaggac
      361 gaggtggccc acacactcac cgagaaccgc gtcctgcaga actccaggca ccccttcctc
      421 acagccctga agtactcttt ccagacccac gaccgcctct gctttgtcat ggagtacgcc
      481 aacgggggcg agctgttctt ccacctgtcc cgggaacgtg tgttctccga ggaccgggcc
      541 cgcttctatg gcgctgagat tgtgtcagcc ctggactacc tgcactcgga gaagaacgtg
      601 gtgtaccggg acctcaagct ggagaacctc atgctggaca aagacgggca cattaagatc
      661 acagacttcg ggctgtgcaa ggagggga
//
