LOCUS       sequence-22352-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Tuttle RL, Gill NS, Pugh W, Lee JP, Koeberlein B, Furth EE, Polonsky
            KS, Naji A, Birnbaum MJ
  TITLE     Regulation of pancreatic beta-cell growth and survival by the 
            serine/threonine protein kinase Akt1/PKBalpha.
  JOURNAL   Nat Med. 2001 Oct . 7(10):1133-7.
  PUBMED    11590437
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2001 Oct . 7(10):1133-7."
COMMENT     Sequence Label: pLNCX1 HA Akt1
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             152..178
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg cggccccaag cttggcgacg cggccgcaga
      121 tctagaaact taagcttcga tcgtcgacat gtacccatac gatgttccag attacgcttc
      181 tagaatgaac gacgtagcca ttgggaagga gggctggctg cacaaacgag gggaatatat
      241 taaaacctgg cggccacgct acttcctcct caagaacgat ggcaccttta ttggctacaa
      301 ggaacggcct caggatgtgg atcagcgaga gtccccactc aacaacttct cagtggcaca
      361 atggcagctg aagaagacag aacggccaag ggccaacacc tttatcatcc gctgcctgga
      421 gtggaccaca gtcattgagc gcaccttcca tggggaaacg cctgaagagc gggaagaatg
      481 ggccaccgcc attcagaatg gggccgaagg actccagaag caggaagaag agacgaagga
      541 cttccgatca gggtcaccca gtgacaactc aggggctgaa gagatggagg tgtccctggc
      601 caagcccaag caccgtgtga ccatgaacga gtttgagtac ctgaaactac tgggcaaggg
      661 cacctttg
//
