LOCUS       sequence-223527-       11497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  puro-selectable lentivirus vector for inducible expression of a 
            UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC,
            Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel 
            MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW
  TITLE     Context-Dependent and Disease-Specific Diversity in Protein 
            Interactions within Stress Granules.
  JOURNAL   Cell. 2018 Jan 25;172(3):590-604.e13. doi: 
            10.1016/j.cell.2017.12.032.
  PUBMED    29373831
REFERENCE   2  (bases 1 to 11497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.12"; journalName: "Cell"; date: "2018-01-25- 
            25"; volume: "172"; issue: "3"; pages: "590-604.e13"
COMMENT     Sequence Label: pLIX403_deltaUBA_UBAP2L_mCherry
FEATURES             Location/Qualifiers
     source          1..11497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..36
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    60..84
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3117..3827
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3262..3280)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3530..3549
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3829..3853)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3864..3884
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3885..3908
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3925..3942
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3952..4462
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4381..4399
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4472..5068
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4472..4491)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4968..4988
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5069..5122
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5123..5740,5741..5746,5747..5782,5783..5785,5786..5821,
                     5822..5824,5825..5860,5861..5869)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5909..5929)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6327..6338)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6543..6776
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6848..6969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6885..6904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6939..6958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7009..7144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7144..7215
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7216..7284,7285..8076)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7434..7453)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8247..8835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8736..8755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8989..9006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9123..9144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9159..9164,9165..9182,9183..9189)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9197..9213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9202..9224
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9221..9237
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9221..9237
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9256..9276
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9258..9276
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9304..9530
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9531..9711
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9758..9883
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10376..10609
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10794..10838
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11051..11168
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(11222..11497,1..39)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    11230..11248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11266..11284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11301..11319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11337..11355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11373..11391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11408..11426
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11444..11462
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagaattgg ctagcatcaa
       61 caagtttgta caaaaaagca ggcttcacca tgatgacatc ggtgggcact aaccgagccc
      121 ggggaaactg ggaacaacct caaaacaagg atggtggcca gacggaatcc aatgaggaag
      181 gcaaagaaaa tcgagaccgg gacagagact atagtcggcg acgtggtggg ccaccaagac
      241 gggggagagg tgccagccgt ggacgagagt ttcgaggtca ggaaaatgga ttggatggca
      301 ccaagagtgg agggccttct ggaagaggaa cagaaagagg cagaaggggc cgtggccgag
      361 gcagaggtgg ctctggtagg cgaggaggaa ggttttctgc tcaaggaatg ggaaccttta
      421 acccagctga ttatgcagag ccagccaata ctgatgataa ctatggcaat agcagcggca
      481 atacgtggaa caacactggc cactttgaac cagatgatgg gacgagtgca tggaggactg
      541 caacagagga gtgggggact gaagattgga atgaagatct ttctgagacc aagatcttca
      601 ctgcctctaa tgtgtcttca gtgcctctgc ctgcggagaa tgtgacaatc actgctggtc
      661 agagaattga ccttgctgtt ctgctgggga agacaccatc tacaatggag aatgattcat
      721 ctaatctgga tccgtctcag gctccttctc tggcccagcc tctggtgttc agtaattcga
      781 agcagactgc catatcacag cctgcttcag ggaacacatt ttctcatcac agtatggtga
      841 gcatgttagg gaaaggattt ggtgatgtcg gtgaagctaa aggcggcagt actacaggct
      901 cccagttctt ggagcaattc aagactgccc aagccctggc tcagttggca gctcagcatt
      961 ctcagtctgg aagcaccacc acctcctctt gggacatggg ctcgacgaca caatccccat
     1021 cactggtgca gtatgatttg aagaacccaa gtgattcagc agtgcacagc ccctttacaa
     1081 agcgccaggc ttttacccca tcttcaacca tgatggaggt gttccttcag gagaagtcac
     1141 ctgcagtggc tacctccaca gctgcacctc cacctccgtc ttctcctctg ccaagcaaat
     1201 ccacatcggc tccacagatg tcgcctggat cttcagacaa ccagtcctct agccctcagc
     1261 cggctcacca gaaactgaaa cagcagaaga aaaaagcctc cttgacttct aagattcctg
     1321 ctctggctgt ggagatgcct ggctcagcag atatctcagg gctaaacctg cagtttgggg
     1381 cattgcagtt tgggtcagag cctgtccttt ctgattatga gtccaccccc accacgagcg
     1441 cctcttcaag ccaggctcca agtagcctgt ataccagcac ggccagtgaa tcatcctcta
     1501 caatttcatc taaccagagt caggagtctg gttatcagag cggcccaatt cagtcgacaa
     1561 cctatacctc ccaaaataat gctcagggcc ctctttatga acagagatcc acacagactc
     1621 ggcggtaccc cagctccatc tcttcatcac cccaaaagga cctgactcag gcaaagaatg
     1681 gcttcagttc tgtgcaggcc acgcagttac agaccacaca atctgttgaa ggtgctacag
     1741 gctctgcagt gaaatctgat tcaccttcca cttctagcat cccccctctc aatgaaacgg
     1801 tatctgcagc ttccttactg acgacaacca atcagcattc atcctccttg ggtggcttga
     1861 gccacagtga ggagattcca aatactacca ccacacaaca cagcagcacg ttatctacgc
     1921 agcagaatac cctttcatca tcaacatctt ctgggcgcac ttcgacatcc actcttttgc
     1981 acacaagtgt ggagagtgag gcgaatctcc attcttcctc cagcactttt tccaccacat
     2041 ccagcacagt ctctgcacct cccccagtgg tcagtgtctc ctccagtctc aatagtggca
     2101 gtagcctggg cctcagccta ggcagcaact ccactgtcac agcctcgact cgaagctcag
     2161 ttgctacgac ttcaggaaaa gctcctccca acctccctcc tggggtcccg ccgttgttgc
     2221 ctaatccgta tattatggct ccagggctgt tacatgccta cccgccacaa gtatatggtt
     2281 atgatgactt gcagatgctt cagacaagat ttccattgga ttactacagc atcccatttc
     2341 ccacacccac tactccgctg actgggaggg atggtagcct ggccagcaac ccttattctg
     2401 gtgacctcac aaagttcggc cgtggggatg cctcctcccc agccccggcc acaaccttgg
     2461 cccaacccca acagaaccag acgcagactc accataccac gcagcagaca ttcctgaacc
     2521 cggcgctgcc tcctggctac agttacacca gcctgccata ctatacaggg gtcccgggcc
     2581 tccccagcac cttccagtat gggcctgctg tgttccctgt ggctcctacc tcttccaagc
     2641 agcatggtgt gaatgtcagt gtgaatgcat cggccacccc tttccaacag ccgagtggat
     2701 atgggtctca tggatacaac actggtgttt cagtcacctc cagtaacacg ggcgtgccag
     2761 atatctcggg ttctgtgtac tccaaaaccc agcagtcctt tgagaaacaa ggttttcatt
     2821 ccggtactcc tgctgcttcc ttcaacttgc cttcagccct aggaagtggg ggccccatca
     2881 atccggccac agctgctgcc tacccacctg ccccctttat gcacattctg accccccatc
     2941 agcagccgca ttctcagatc cttcaccatc acctgcagca ggatggccag acgggcagcg
     3001 ggcaacgtag ccagaccagc tccatcccgc agaagcccca gaccaacaag tctgcctaca
     3061 acagctacag ctggggggcc aacaccggtg tggacaccgg caacggttcc gctagcatgg
     3121 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     3181 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     3241 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     3301 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     3361 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     3421 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     3481 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     3541 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     3601 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     3661 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     3721 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     3781 ccgagggccg ccactccacc ggcggcatgg acgagctcta caagtaaaac ccagctttct
     3841 tgtacaaagt ggttgcaacg cgtgaaaacc tgtattttca gtccgattac aaggacgacg
     3901 atgacaagta ggcatagacc ggtccaccac caccaccacc actaaggatc cggggttggg
     3961 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     4021 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     4081 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     4141 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     4201 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     4261 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     4321 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     4381 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     4441 aatcaccgac ctctctcccc agcaattcac catgaccgag tacaagccca cggtgcgcct
     4501 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     4561 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     4621 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     4681 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     4741 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     4801 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     4861 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     4921 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     4981 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5041 ctggtgcatg acccgcaagc ccggtgccga aggtagaggt tctctcctca cttgtggtga
     5101 tgttgaagaa aaccctggtc caatgtctag actggacaag agcaaagtca taaacggagc
     5161 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     5221 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     5281 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     5341 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     5401 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     5461 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     5521 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     5581 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     5641 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     5701 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     5761 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     5821 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga
     5881 tcgatccaag atatcgtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5941 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6001 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6061 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6121 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6181 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6241 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     6301 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6361 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6421 tctccctttg ggccgcctcc ccgcctgttt cgcctcggcg tccggactag aggtaccttt
     6481 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6541 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6601 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6661 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6721 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6781 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6841 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6961 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     7021 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7081 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7141 ggcctttcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7201 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7261 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7321 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7381 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7441 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7501 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7561 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7621 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7681 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7741 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7801 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7861 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7921 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7981 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8041 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8101 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8161 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9001 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9061 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     9121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     9181 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     9241 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     9301 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     9361 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     9421 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     9481 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     9541 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9601 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9661 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     9721 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     9781 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     9841 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     9901 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     9961 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    10021 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    10081 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    10141 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    10201 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
    10261 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
    10321 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
    10381 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    10441 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
    10501 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
    10561 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    10621 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
    10681 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
    10741 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
    10801 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    10861 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    10921 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
    10981 ttcagaccca cctcccaacc ccgaggggac aattctcgac ctcgagacaa atggcagtat
    11041 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    11101 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    11161 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcga atcgatatgt
    11221 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
    11281 gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
    11341 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
    11401 gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
    11461 gaacgtatgt cgaggtaggc gtgtacggtg ggaggcc
//
