LOCUS       sequence-223528-       11764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  puro-selectable lentivirus vector for inducible expression of a 
            UBAP2L-mCherry fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11764)
  AUTHORS   Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC,
            Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel 
            MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW
  TITLE     Context-Dependent and Disease-Specific Diversity in Protein 
            Interactions within Stress Granules.
  JOURNAL   Cell. 2018 Jan 25;172(3):590-604.e13. doi: 
            10.1016/j.cell.2017.12.032.
  PUBMED    29373831
REFERENCE   2  (bases 1 to 11764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.12"; journalName: "Cell"; date: "2018-01-25- 
            25"; volume: "172"; issue: "3"; pages: "590-604.e13"
COMMENT     Sequence Label: pLIX403_UBAP2L_mCherry
FEATURES             Location/Qualifiers
     source          1..11764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3871..4581
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4016..4034)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4284..4303
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4583..4607)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4618..4638
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4639..4662
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4679..4696
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4706..5216
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5135..5153
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5226..5822
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5226..5245)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5722..5742
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5823..5876
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5877..6494,6495..6500,6501..6536,6537..6539,6540..6575,
                     6576..6578,6579..6614,6615..6623)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6663..6683)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7081..7092)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7297..7530
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7602..7723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7639..7658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7693..7712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7763..7898
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7898..7969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7970..8038,8039..8830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8188..8207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9001..9589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9490..9509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9743..9760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9877..9898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9913..9918,9919..9936,9937..9943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9951..9967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9956..9978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9975..9991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9975..9991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10010..10030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10012..10030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10058..10284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10285..10465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10512..10637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11130..11363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11548..11592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc ttcaccatga tgacatcggt gggcactaac
      601 cgagcccggg gaaactggga acaacctcaa aaccaaaacc agacacagca caagcagcgg
      661 ccacaggcca ctgcagaaca aattagactt gcacagatga tttcggacca taatgatgct
      721 gactttgagg agaaggtgaa acaattgatt gatattacag gcaagaacca ggatgaatgt
      781 gtgattgctt tgcatgactg caatggagat gtcaacagag ctatcaatgt tcttctggaa
      841 ggaaacccag acacgcattc ctgggagatg gtcgggaaga agaagggagt ctcaggccag
      901 aaggatggtg gccagacgga atccaatgag gaaggcaaag aaaatcgaga ccgggacaga
      961 gactatagtc ggcgacgtgg tgggccacca agacggggga gaggtgccag ccgtggacga
     1021 gagtttcgag gtcaggaaaa tggattggat ggcaccaaga gtggagggcc ttctggaaga
     1081 ggaacagaaa gaggcagaag gggccgtggc cgaggcagag gtggctctgg taggcgagga
     1141 ggaaggtttt ctgctcaagg aatgggaacc tttaacccag ctgattatgc agagccagcc
     1201 aatactgatg ataactatgg caatagcagc ggcaatacgt ggaacaacac tggccacttt
     1261 gaaccagatg atgggacgag tgcatggagg actgcaacag aggagtgggg gactgaagat
     1321 tggaatgaag atctttctga gaccaagatc ttcactgcct ctaatgtgtc ttcagtgcct
     1381 ctgcctgcgg agaatgtgac aatcactgct ggtcagagaa ttgaccttgc tgttctgctg
     1441 gggaagacac catctacaat ggagaatgat tcatctaatc tggatccgtc tcaggctcct
     1501 tctctggccc agcctctggt gttcagtaat tcgaagcaga ctgccatatc acagcctgct
     1561 tcagggaaca cattttctca tcacagtatg gtgagcatgt tagggaaagg atttggtgat
     1621 gtcggtgaag ctaaaggcgg cagtactaca ggctcccagt tcttggagca attcaagact
     1681 gcccaagccc tggctcagtt ggcagctcag cattctcagt ctggaagcac caccacctcc
     1741 tcttgggaca tgggctcgac gacacaatcc ccatcactgg tgcagtatga tttgaagaac
     1801 ccaagtgatt cagcagtgca cagccccttt acaaagcgcc aggcttttac cccatcttca
     1861 accatgatgg aggtgttcct tcaggagaag tcacctgcag tggctacctc cacagctgca
     1921 cctccacctc cgtcttctcc tctgccaagc aaatccacat cggctccaca gatgtcgcct
     1981 ggatcttcag acaaccagtc ctctagccct cagccggctc accagaaact gaaacagcag
     2041 aagaaaaaag cctccttgac ttctaagatt cctgctctgg ctgtggagat gcctggctca
     2101 gcagatatct cagggctaaa cctgcagttt ggggcattgc agtttgggtc agagcctgtc
     2161 ctttctgatt atgagtccac ccccaccacg agcgcctctt caagccaggc tccaagtagc
     2221 ctgtatacca gcacggccag tgaatcatcc tctacaattt catctaacca gagtcaggag
     2281 tctggttatc agagcggccc aattcagtcg acaacctata cctcccaaaa taatgctcag
     2341 ggccctcttt atgaacagag atccacacag actcggcggt accccagctc catctcttca
     2401 tcaccccaaa aggacctgac tcaggcaaag aatggcttca gttctgtgca ggccacgcag
     2461 ttacagacca cacaatctgt tgaaggtgct acaggctctg cagtgaaatc tgattcacct
     2521 tccacttcta gcatcccccc tctcaatgaa acggtatctg cagcttcctt actgacgaca
     2581 accaatcagc attcatcctc cttgggtggc ttgagccaca gtgaggagat tccaaatact
     2641 accaccacac aacacagcag cacgttatct acgcagcaga ataccctttc atcatcaaca
     2701 tcttctgggc gcacttcgac atccactctt ttgcacacaa gtgtggagag tgaggcgaat
     2761 ctccattctt cctccagcac tttttccacc acatccagca cagtctctgc acctccccca
     2821 gtggtcagtg tctcctccag tctcaatagt ggcagtagcc tgggcctcag cctaggcagc
     2881 aactccactg tcacagcctc gactcgaagc tcagttgcta cgacttcagg aaaagctcct
     2941 cccaacctcc ctcctggggt cccgccgttg ttgcctaatc cgtatattat ggctccaggg
     3001 ctgttacatg cctacccgcc acaagtatat ggttatgatg acttgcagat gcttcagaca
     3061 agatttccat tggattacta cagcatccca tttcccacac ccactactcc gctgactggg
     3121 agggatggta gcctggccag caacccttat tctggtgacc tcacaaagtt cggccgtggg
     3181 gatgcctcct ccccagcccc ggccacaacc ttggcccaac cccaacagaa ccagacgcag
     3241 actcaccata ccacgcagca gacattcctg aacccggcgc tgcctcctgg ctacagttac
     3301 accagcctgc catactatac aggggtcccg ggcctcccca gcaccttcca gtatgggcct
     3361 gctgtgttcc ctgtggctcc tacctcttcc aagcagcatg gtgtgaatgt cagtgtgaat
     3421 gcatcggcca cccctttcca acagccgagt ggatatgggt ctcatggata caacactggt
     3481 gtttcagtca cctccagtaa cacgggcgtg ccagatatct cgggttctgt gtactccaaa
     3541 acccagcagt cctttgagaa acaaggtttt cattccggta ctcctgctgc ttccttcaac
     3601 ttgccttcag ccctaggaag tgggggcccc atcaatccgg ccacagctgc tgcctaccca
     3661 cctgccccct ttatgcacat tctgaccccc catcagcagc cgcattctca gatccttcac
     3721 catcacctgc agcaggatgg ccagacgggc agcgggcaac gtagccagac cagctccatc
     3781 ccgcagaagc cccagaccaa caagtctgcc tacaacagct acagctgggg ggccaacacc
     3841 ggtgtggaca ccggcaacgg ttccgctagc atggtgagca agggcgagga ggataacatg
     3901 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     3961 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     4021 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     4081 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     4141 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     4201 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     4261 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     4321 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     4381 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     4441 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     4501 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     4561 atggacgagc tctacaagta aaacccagct ttcttgtaca aagtggttgc aacgcgtgaa
     4621 aacctgtatt ttcagtccga ttacaaggac gacgatgaca agtaggcata gaccggtcca
     4681 ccaccaccac caccactaag gatccggggt tggggttgcg ccttttccaa ggcagccctg
     4741 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     4801 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     4861 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     4921 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     4981 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     5041 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     5101 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     5161 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccagcaat
     5221 tcaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     5281 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     5341 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     5401 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     5461 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     5521 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     5581 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     5641 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     5701 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     5761 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     5821 ccgaaggtag aggttctctc ctcacttgtg gtgatgttga agaaaaccct ggtccaatgt
     5881 ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta
     5941 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     6001 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     6061 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     6121 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     6181 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     6241 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     6301 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     6361 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     6421 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     6481 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     6541 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     6601 accttgacat gctccccggg taactaagta aggatcgatc caagatatcg tattcttaac
     6661 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6721 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6781 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6841 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6901 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6961 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     7021 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7081 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7141 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7201 gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac aaggcagctg
     7261 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7321 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7381 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7441 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7501 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7561 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7621 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7681 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7741 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7801 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7861 gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt atccgctcat
     7921 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7981 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8041 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8101 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8161 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8221 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8281 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8341 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8401 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8461 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8521 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8581 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8641 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8701 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8761 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8821 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8881 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8941 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9001 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9061 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
    10021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
    10081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
    10141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
    10201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
    10261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
    10321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
    10501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
    10561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    10621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
    10681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
    10741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    10801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    10861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    10921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    10981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
    11041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    11101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    11161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    11221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    11281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    11341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    11401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    11461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    11521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    11581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    11641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    11701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    11761 ggac
//
