LOCUS       sequence-223558-        7200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make
            CRISPR construct . For use in plants and compatible with the 
            MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Wang X, Ye L, Lyu M, Ursache R, Loytynoja A, Mahonen AP
  TITLE     An inducible genome editing system for plants.
  JOURNAL   Nat Plants. 2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 
            10.1038/s41477-020-0695-2.
  PUBMED    32601420
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 
            10.1038/s41477-020-0695-2."
COMMENT     Sequence Label: p221z-CAS9p-TagRFP-t35s
FEATURES             Location/Qualifiers
     source          1..7200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             677..697
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             722..4822
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             722..4822
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             1908..1916
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4823..5512
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     CDS             5516..5563
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    complement(5796..5895)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5912..5931)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5913..5931)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5936..5952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5936..5952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(6020..6394)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(6413..6460)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      6550..7138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7039..7058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcg atggctccta agaagaagcg gaaggttggt attcacgggg tgcctgcggc
      721 tgacaagaag tactccatcg gcctcgacat cggcaccaac agcgtcggct gggcggtgat
      781 caccgacgag tacaaggtcc cgtccaagaa gttcaaggtc ctgggcaaca ccgaccgcca
      841 ctccatcaag aagaacctca tcggcgccct cctcttcgac tccggcgaga cggcggaggc
      901 gacccgcctc aagcgcaccg cccgccgccg ctacacccgc cgcaagaacc gcatctgcta
      961 cctccaggag atcttctcca acgagatggc gaaggtcgac gactccttct tccaccgcct
     1021 cgaggagtcc ttcctcgtgg aggaggacaa gaagcacgag cgccacccca tcttcggcaa
     1081 catcgtcgac gaggtcgcct accacgagaa gtaccccact atctaccacc ttcgtaagaa
     1141 gcttgttgac tctactgata aggctgatct tcgtctcatc taccttgctc tcgctcacat
     1201 gatcaagttc cgtggtcact tccttatcga gggtgacctt aaccctgata actccgacgt
     1261 ggacaagctc ttcatccagc tcgtccagac ctacaaccag ctcttcgagg agaaccctat
     1321 caacgcttcc ggtgtcgacg ctaaggcgat cctttccgct aggctctcca agtccaggcg
     1381 tctcgagaac ctcatcgccc agctccctgg tgagaagaag aacggtcttt tcggtaacct
     1441 catcgctctc tccctcggtc tgacccctaa cttcaagtcc aacttcgacc tcgctgagga
     1501 cgctaagctt cagctctcca aggataccta cgacgatgat ctcgacaacc tcctcgctca
     1561 gattggagat cagtacgctg atctcttcct tgctgctaag aacctctccg atgctatcct
     1621 cctttcggat atccttaggg ttaacactga gatcactaag gctcctcttt ctgcttccat
     1681 gatcaagcgc tacgacgagc accaccagga cctcaccctc ctcaaggctc ttgttcgtca
     1741 gcagctcccc gagaagtaca aggagatctt cttcgaccag tccaagaacg gctacgccgg
     1801 ttacattgac ggtggagcta gccaggagga gttctacaag ttcatcaagc caatccttga
     1861 gaagatggat ggtactgagg agcttctcgt taagcttaac cgtgaggacc tccttaggaa
     1921 gcagaggact ttcgataacg gctctatccc tcaccagatc caccttggtg agcttcacgc
     1981 catccttcgt aggcaggagg acttctaccc tttcctcaag gacaaccgtg agaagatcga
     2041 gaagatcctt actttccgta ttccttacta cgttggtcct cttgctcgtg gtaactcccg
     2101 tttcgcttgg atgactagga agtccgagga gactatcacc ccttggaact tcgaggaggt
     2161 tgttgacaag ggtgcttccg cccagtcctt catcgagcgc atgaccaact tcgacaagaa
     2221 cctccccaac gagaaggtcc tccccaagca ctccctcctc tacgagtact tcacggtcta
     2281 caacgagctc accaaggtca agtacgtcac cgagggtatg cgcaagcctg ccttcctctc
     2341 cggcgagcag aagaaggcta tcgttgacct cctcttcaag accaaccgca aggtcaccgt
     2401 caagcagctc aaggaggact acttcaagaa gatcgagtgc ttcgactccg tcgagatcag
     2461 cggcgttgag gaccgtttca acgcttctct cggtacctac cacgatctcc tcaagatcat
     2521 caaggacaag gacttcctcg acaacgagga gaacgaggac atcctcgagg acatcgtcct
     2581 cactcttact ctcttcgagg atagggagat gatcgaggag aggctcaaga cttacgctca
     2641 tctcttcgat gacaaggtta tgaagcagct caagcgtcgc cgttacaccg gttggggtag
     2701 gctctcccgc aagctcatca acggtatcag ggataagcag agcggcaaga ctatcctcga
     2761 cttcctcaag tctgatggtt tcgctaacag gaacttcatg cagctcatcc acgatgactc
     2821 tcttaccttc aaggaggata ttcagaaggc tcaggtgtcc ggtcagggcg actctctcca
     2881 cgagcacatt gctaaccttg ctggttcccc tgctatcaag aagggcatcc ttcagactgt
     2941 taaggttgtc gatgagcttg tcaaggttat gggtcgtcac aagcctgaga acatcgtcat
     3001 cgagatggct cgtgagaacc agactaccca gaagggtcag aagaactcga gggagcgcat
     3061 gaagaggatt gaggagggta tcaaggagct tggttctcag atccttaagg agcaccctgt
     3121 cgagaacacc cagctccaga acgagaagct ctacctctac tacctccaga acggtaggga
     3181 tatgtacgtt gaccaggagc tcgacatcaa caggctttct gactacgacg tcgaccacat
     3241 tgttcctcag tctttcctta aggatgactc catcgacaac aaggtcctca cgaggtccga
     3301 caagaacagg ggtaagtcgg acaacgtccc ttccgaggag gttgtcaaga agatgaagaa
     3361 ctactggagg cagcttctca acgctaagct cattacccag aggaagttcg acaacctcac
     3421 gaaggctgag aggggtggcc tttccgagct tgacaaggct ggtttcatca agaggcagct
     3481 tgttgagacg aggcagatta ccaagcacgt tgctcagatc ctcgattcta ggatgaacac
     3541 caagtacgac gagaacgaca agctcatccg cgaggtcaag gtgatcaccc tcaagtccaa
     3601 gctcgtctcc gacttccgca aggacttcca gttctacaag gtccgcgaga tcaacaacta
     3661 ccaccacgct cacgatgctt accttaacgc tgtcgttggt accgctctta tcaagaagta
     3721 ccctaagctt gagtccgagt tcgtctacgg tgactacaag gtctacgacg ttcgtaagat
     3781 gatcgccaag tccgagcagg agatcggcaa ggccaccgcc aagtacttct tctactccaa
     3841 catcatgaac ttcttcaaga ccgagatcac cctcgccaac ggcgagatcc gcaagcgccc
     3901 tcttatcgag acgaacggtg agactggtga gatcgtttgg gacaagggtc gcgacttcgc
     3961 tactgttcgc aaggtccttt ctatgcctca ggttaacatc gtcaagaaga ccgaggtcca
     4021 gaccggtggc ttctccaagg agtctatcct tccaaagaga aactcggaca agctcatcgc
     4081 taggaagaag gattgggacc ctaagaagta cggtggtttc gactccccta ctgtcgccta
     4141 ctccgtcctc gtggtcgcca aggtggagaa gggtaagtcg aagaagctca agtccgtcaa
     4201 ggagctcctc ggcatcacca tcatggagcg ctcctccttc gagaagaacc cgatcgactt
     4261 cctcgaggcc aagggctaca aggaggtcaa gaaggacctc atcatcaagc tccccaagta
     4321 ctctcttttc gagctcgaga acggtcgtaa gaggatgctg gcttccgctg gtgagctcca
     4381 gaagggtaac gagcttgctc ttccttccaa gtacgtgaac ttcctctacc tcgcctccca
     4441 ctacgagaag ctcaagggtt cccctgagga taacgagcag aagcagctct tcgtggagca
     4501 gcacaagcac tacctcgacg agatcatcga gcagatctcc gagttctcca agcgcgtcat
     4561 cctcgctgac gctaacctcg acaaggtcct ctccgcctac aacaagcacc gcgacaagcc
     4621 catccgcgag caggccgaga acatcatcca cctcttcacg ctcacgaacc tcggcgcccc
     4681 tgctgctttc aagtacttcg acaccaccat cgacaggaag cgttacacgt ccaccaagga
     4741 ggttctcgac gctactctca tccaccagtc catcaccggt ctttacgaga ctcgtatcga
     4801 cctttcccag cttggtggtg atatgagcga gctgattaag gagaacatgc acatgaagct
     4861 gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa
     4921 gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt
     4981 cgccttcgac atcctggcta ccagcttcat gtacggcagc agaaccttca tcaaccacac
     5041 ccagggcatc cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt
     5101 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     5161 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat
     5221 gcagaagaaa acactcggct gggaggccaa caccgagatg ctgtaccccg ctgacggcgg
     5281 cctggaaggc agaagcgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa
     5341 cttcaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta
     5401 ctatgtggac cacagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca
     5461 gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc acaagaagcg
     5521 tcctgctgcc accaaaaagg ccggacaggc taagaaaaag aagtaggatc cacctgatct
     5581 agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag
     5641 aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg
     5701 tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata
     5761 aaatttctaa ttcctaaaac caaaatccag tgaccaccca gctttcttgt acaaagttgg
     5821 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     5881 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     5941 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     6001 taaaataata tcatcatgat cagtcctgct cctcggccac gaagtgcacg cagttgccgg
     6061 ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg
     6121 gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt
     6181 ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg
     6241 cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt
     6301 cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg
     6361 tgagcaccgg aacggcactg gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt
     6421 atactatgcc gatatactat gccgatgatt aattgtcaac acgtgctgat catgaccaaa
     6481 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6541 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6601 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6661 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6721 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6781 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6841 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6901 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6961 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7021 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7081 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7141 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
