LOCUS       sequence-223572-        7168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and 
            mCherry in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Slepak TI, Salay LD, Lemmon VP, Bixby JL
  TITLE     Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal 
            organization, and neuronal morphology.
  JOURNAL   Cytoskeleton (Hoboken). 2012 Jul;69(7):514-27. doi: 
            10.1002/cm.21021. Epub 2012 Mar 7.
  PUBMED    22359282
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cm.21021"; 
            journalName: "Cytoskeleton (Hoboken)"; date: "2012-07"; volume: 
            "69"; issue: "7"; pages: "514-27"
COMMENT     Sequence Label: pAAV-DYRK3-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..7168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     678..698
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     724..748
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          876..1254
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             3112..3165
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3193..3900
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3335..3353)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3603..3622
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3915..4391
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4114..4133)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4431..4571
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4431..4560
                     /label=AAV2 ITR
     rep_origin      4646..5101
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4733..4752)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4943..4964
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5118..5137)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5237..5259
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5297..5315)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5383..5487
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5488..5556,5557..6348)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5706..5725)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6519..7107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7008..7027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
      841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
     1321 cgattgaatt caatgggagg cgcagcccgc gatcgcggga ggaaggacgc ggcgctgccg
     1381 ggggccgggc tcccgccgca gcagcggagg ttgggggatg gtgtctatga tactttcatg
     1441 atgatagatg aaaccaaggg cccaccctat tcggacacat tcagcaaccc ctctgaagca
     1501 cctgtctcca gaaggctaaa tattaccact gagccactca cgagaggcca cactcagcac
     1561 tttgtgaatg gaagtgagat gaaggtggaa caactgtttc aagaatttgg caacagaaga
     1621 tccaatactc tccagtcaga tggcatcagc aactcggaaa agtcttctcc ggcttctcag
     1681 gggaaaagtt cagaaagcct gagcgcggtg aagtgcaacc tttcctccag gccgtctaag
     1741 gtgctgccgc tgactcctga gcaagcgctg aagcagtata aacaccacct cactgcctac
     1801 gagaagctgg agatcgtcag ctacccagaa atctactttg tgggtccgaa tgccaaaaag
     1861 cggcagggag ttattggtgg tcccaataac ggtggctacg acgatgcgga tggggcctat
     1921 attcatgtgc cccgggacca tctggcttac cgctatgagg tgctgaaaat catcggcaag
     1981 gggagttttg gacaggtagc ccgggtctat gatcacaaac ttcggcagta cgtggccctg
     2041 aaaatggtgc gcaatgagaa acgcttccat cgccaggcag ccgaggagat ccggatcttg
     2101 gagcatctta aaaagcaaga caaaactggt agcatgaacg tcatccacat gctagaaagt
     2161 ttcaccttcc ggaaccacgt gtgcatggcc tttgaactgc taagcataga cctgtatgag
     2221 cttattaaaa aaaacaagtt ccagggcttc agcgtccagt tggttcggaa gttcgcccag
     2281 tccatcctac agtccctgga cgctctgcac aaaaacaaga tcatccactg cgacctgaag
     2341 ccggaaaaca ttctcctgaa acaccacggc cgaagcgcca ccaaggtcat cgactttggc
     2401 tccagctgct tcgagtatca gaagctttac acgtatatcc agtcccgctt ctacagagcc
     2461 ccagagatca tcctggggtg ccgctacagc accccgatcg acatatggag ctttggttgc
     2521 atccttgcag aacttttgac aggacagccc ctgttccctg gagaggacga aggagaccag
     2581 ttggcctgca tgatagagtt gctaggaatg ccaccgcaga aacttctgga gcaatccaag
     2641 cgtgccaagt actttattaa ctccaaaggc ttgcctcgat actgctccgt atctacccag
     2701 acggacggga gggtggtgct tctcgggggt cgctcacgca ggggtaaaaa gcgaggcccg
     2761 ccaggcagca aagactgggc aaccgcactg aagggctgtg gtgactactt gttcatagag
     2821 tttctgaaac gatgcctcca gtgggacccc tctgcccgcc tcaccccggc tcaagcatta
     2881 agacatcctt ggattagcaa gtctacaccc aaacctctca ccatggacaa ggtgccaggg
     2941 aagcgggtag ttaaccctac aaatgctttc cagggactgg gttccaagct gcctccagtc
     3001 gttgggatag ccagtaagct taaagctaac ctaatgtccg aaaccagtgg tagtatacct
     3061 ctgtgcagtg tattgccaaa gctgattagc ctcgaccggg gtaccaccgg tgaaggccgc
     3121 ggcagcctgc tgacctgcgg cgatgtggaa gaaaacccgg gcccggcgcc gggcagcact
     3181 agtcggaagc ttgtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     3241 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     3301 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     3361 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     3421 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     3481 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     3541 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     3601 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     3661 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     3721 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     3781 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     3841 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     3901 ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     3961 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     4021 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     4081 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     4141 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     4201 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     4261 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     4321 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     4381 tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct
     4441 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     4501 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     4561 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4621 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4681 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4741 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4801 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4861 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4921 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4981 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5041 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     5101 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     5161 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5221 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     5281 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5341 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5401 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5461 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5521 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5581 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5641 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5701 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5761 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5821 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5881 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5941 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6001 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6061 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6121 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6181 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6241 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6301 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6361 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6421 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6661 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7081 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7141 gccttttgct ggccttttgc tcacatgt
//
