LOCUS       sequence-22358-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  TITLE     Birnbaum plasmids
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAMPK gamma1
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             118..135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             139..171
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(175..183,184..198)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacttaagc ttaccatggg gggttctcat
      121 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
      181 tacgacgatg acgataaggt accaggatcc agtgtggtgg aattcccggg tcgacccacg
      241 cgtccgtggc gggcgctagc aatggagtcg gttgctgcag agagctcgcc agctctagag
      301 aatgaacact ttcaagagac cccggaatca aacaatagtg tgtatacttc cttcatgaag
      361 tctcatcgct gctatgacct aattcccaca agttccaagt tggtggtatt tgacacttcg
      421 ctacaggtaa agaaagcctt ttttgccctg gtgaccaatg gtgttcgtgc cgcccctttg
      481 tgggacagta agaagcagag ttttgtgggc atgctgacca tcaccgactt catcaacatt
      541 ttgcaccgat actataagtc agccctggtg cagatctacg aactggagga gcacaagata
      601 gagacgtgga gagaggtgta cctgcaggac tcctttaagc cacttgtctg catctctcca
      661 aatgccagct tgtttgatgc tgtctctt
//
