LOCUS       sequence-22359-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  TITLE     Birnbaum plasmids
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAMPK gamma1 H150R
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             132..158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agctctctgg ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc
      121 tggaattcat gtacccatac gatgttccag attacgcaat ggagtcggtt gctgcagaga
      181 gctcgccagc tctagagaat gaacactttc aagagacccc ggaatcaaac aatagtgtgt
      241 atacttcctt catgaagtct catcgctgct atgacctaat tcccacaagt tccaagttgg
      301 tggtatttga cacttcgcta caggtaaaga aagccttttt tgccctggtg accaatggtg
      361 ttcgtgccgc ccctttgtgg gacagtaaga agcagagttt tgtgggcatg ctgaccatca
      421 ccgacttcat caacattttg caccgatact ataagtcagc cctggtgcag atctacgaac
      481 tggaggagca caagatagag acgtggagag aggtgtacct gcaggactcc tttaagccac
      541 ttgtctgcat ctctccaaat gccagcttgt ttgatgctgt ctcttcatta attcgaaata
      601 agatccgcag gctcccagtt atcgacccag agtcaggcaa caccttgtac atccttactc
      661 acaagcggat cctcaagttc ctcaagttgt ttatcaccga gttccccaag ccggagttca
      721 tgtctaagtc tctccaagag ctgcagattg gcacctatgc caatattgc
//
