LOCUS       sequence-223595-       13559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Knockel J, Dundas K, Yang ASP, Galaway F, Metcalf T, Gemert GV, 
            Sauerwein RW, Rayner JC, Billker O, Wright GJ
  TITLE     Systematic Identification of Plasmodium Falciparum Sporozoite 
            Membrane Protein Interactions Reveals an Essential Role for the p24 
            Complex in Host Infection.
  JOURNAL   Mol Cell Proteomics. 2021;20:100038. doi: 10.1074/mcp.RA120.002432. 
            Epub 2021 Jan 27.
  PUBMED    33515807
REFERENCE   2  (bases 1 to 13559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2021"; volume: "20:100038. doi"; pages: " 
            10.1074/mcp.RA120.002432. Epub 2021 Jan 27"
COMMENT     Sequence Label: MAEBL-COMP-blac-flag-his
FEATURES             Location/Qualifiers
     source          1..13559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7093..7878
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             7885..7950
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7957..7974
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    8010..8065
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8064..8083)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10301..10319)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10387..10491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10492..10560,10561..11352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11523..12111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12012..12031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12265..12282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12399..12420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12435..12440,12441..12458,12459..12465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12473..12489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12478..12500
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12497..12513
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12497..12513
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        12784..13163
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13164..13367
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13318..13338
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     13364..13388
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccatc
      421 aacaaccctc aagaggaatt catggaccgc ttcgacatca acaagaacca cgtgaacatc
      481 aagtggtcca acagcggcat ccacggcaag ggcaagttta agtacgagat cgaggaacgg
      541 gacgtgctga gcgagggcaa cgaggccgag aagtccacca tctgccccaa tcacgtgaaa
      601 gagggcacct acaagctggg ctgccccgac tacggcaaga ccttcctgat gggcttcgag
      661 gacaacaagt acagcgaaga gttcctgaac gagatcagct tcggcttcct gaacaagaag
      721 tacaagctgc ccatcgagat ccctctgaac aaggccggac tgagcatgta ccagggcctg
      781 ttcaagcggt gcccctacaa caagaaacac tacagcatga tcaagaacga gaacgagtac
      841 gacatgtgct ttcggaagtt ctacaacaac gccaatatcg ccacccggat ctacaagcgg
      901 ggcaagcaga accggaagta catctacttc agcagccacg gcctgggcgg cagactgggc
      961 gccaacattg aggaacccct gcacaagtac aagaacgacg agcactacgt gaccaagaag
     1021 atgagatacc ccgagaagat caagaatctg ttcgactgca gcatctacag ccactgcatc
     1081 ggcccctgcc tgtacaagga cttcaacaat gcctgctttc tgaacctgcc catcctgttc
     1141 aaccaccaga ccaaagaatg cgtgatcatc ggcacccacg aggaaaagcg gatccacaac
     1201 tgccagagcg gcagcaccga ccagaatatc cagcggtgct tcctgcccgt gaagaaagaa
     1261 aagggcaacc agtggaccta cgccagcagc ttcatccgga ccgattacat gaccaagtgc
     1321 cctccccggt tcccactgaa ccacgccatg ttcggctact tcaactacgc caccggcaag
     1381 tgcgaaacct actacatgaa ccacgagaag cggaccctga gcttcagcaa gtgcatcgag
     1441 acactgttca acaacatcaa gaagcaggac gacgttaaca acgccgcctt cctgtggggc
     1501 gtgtggacca tcgagaacaa tgccaacgaa aagaccaacc tggccagcat ggacaatacc
     1561 ggcagctgct acttcctgaa gaaaaagcct acctgcgtgc tgaaaaaaga gaaccacttc
     1621 agcttcacca tcctgacggc caacagcttc aacctgaacc agaacatcat ctaccccgag
     1681 ctgaagaaca acgctgccaa agagggctcc agcctgatcc acttcaagga ccccaagcag
     1741 accaacaagc gggtgctgta cgagaacaac aaaaagtcca agcggaacgt gcggaccaag
     1801 atcaacagcg tgaacccctt taccatcccc agcaccctga acaccaacga gaaagaagag
     1861 tataacaaaa acgaagtgaa gaactacccc aacaacaaca ttgcctacat ccagaaggtg
     1921 cacagctcct tcgtgaacaa ccggttcaac atccacagcg actccagctt caagcccatc
     1981 cacaagatga tccagatgaa catctacacc gacaacaagc tgtacaacaa caataaccag
     2041 aagattaagg acagcaataa caacatgaac ggcatgagcg tgacccagcg gagccccagc
     2101 atcggcgaga accagaatgg caaccctcag caaaagtaca tggaaagatt cgacatcccg
     2161 aacaaccaca tcttcatcga gtggcagaaa gaaggcgagt acggcaacga cgagttcaag
     2221 tacaacatca tcagcaacaa ggctgccggc accagccaga gcctgttcca caactacaag
     2281 gacaagacct gccctaacca cgtgtacgag ggacgggccc acggcagctg tcccaattac
     2341 ggcaaggcca tcatcgtgca gaacctgctg ggcgaggaat acgacaagaa cttcaatctg
     2401 aactttctca acgagatccg cacgggctac ctcaacaagt atttcaagaa ggacgtcgag
     2461 atctcctacg agaactccgg aatcgccatg cacaacaata tgctgcggag ctgccccgtg
     2521 catgagaacg aagagaagct gttcagcgtc aagaccgact acaattacaa gatgtgcaag
     2581 agcaagatct tcagcaacag gttcaccatg aaggaatatg accccaagac ccggctgttc
     2641 atgtactacg gcctgtatgg cctgggagga cggctgggag ccaatatcaa gcgcgataag
     2701 cagaaagaga agaagtacga ggataatatc gccctcccca tgaagaaccc cgccctgatt
     2761 aagaacctgt ttgattgctc catctactcc tactgcctgg gcccctgtct ggaaaactcc
     2821 ttcggcaaca agtgcttcag aaacctgccc gcctactaca atcacctgac caacgagtgt
     2881 gtgatcctgg gaacacacga gcaagagcgg accaacagct gcagacgcac caaagaggaa
     2941 aagaagaagc ccaattgcca gatcctgaga aagaccaccg acagcaagga ctggacatac
     3001 gtgtccagct ttatccggcc tgactacgag acaaagtgcc cacctagata cccactgaag
     3061 tccaaggtgt tcggcacctt cgaccagaaa accggcaaat gcaagagcct gatggacaag
     3121 gcctacgaag tgggaatcaa caagttctcc gtgtgcctgg aatacctgtt cctggtgtcc
     3181 cctaaggacc tgtacaactc cggcagaaac aactactggg gcatctgggc cgctgaccac
     3241 tccgtgaacg aaaacaatat cgagatcgcc aacgggaagt gctaccacct ggtggtcaag
     3301 cccacctgtg tgatcgacaa agaaaatcac ttctccttca ctgccctgac cgccaatacc
     3361 gtggacttca atcaggccgt gaatatccgg aagatcgaag aactgaccga gtacggaaac
     3421 aatgacgacg tcctgaaaga gaaagagatc aacaacgagc ccatcgacaa cgtgaacgag
     3481 gccaagatta accgcaagag ccatgtgaac agcatggaac ggaacaagcc cagctacaag
     3541 gaaaatgagt acgaccagat ggaaaagaac gtcgaggacg agacatacag cgaggaattc
     3601 ggcctgtttg aggaagccag aaagaccgag acaggcagaa tcgaggaaga gtccaagaaa
     3661 aaagaagcta tgaagcgggc cgaggacgcc agacggatcg aagaggcccg cagagccgaa
     3721 gatgccagaa gaattgaaga ggctcggagg gctgaggatg ccaggcgggt ggaaatcgcc
     3781 cgcagggtgg aagatgctcg gcggatcgag atttctcgca gagctgagga cgctaagagg
     3841 atcgaggccg ctcggagagc catcgaagtg cggagagccg agctgcggaa ggccgaggat
     3901 gctcggagaa ttgaggccgc cagaagatac gaaaacgagc ggagaatcga agaagcgcgg
     3961 agatacgagg atgagaagcg catcgaagcc gtgaagaggg ccgaagaagt tcggaaggac
     4021 gaagaagagg ccaaaagagc cgaaaaagag cgcaacaacg aagaaatccg caagttcgag
     4081 gaagcccgga tggcccactt cgccaggcgg caggccgcca ttaaggccga agagaaacgc
     4141 aaggccgacg agctgaaaaa ggcggaagaa aagaaaaagg ccgatgaact gaagaagtct
     4201 gaagagaaaa agaaagcgga cgaactcaag aagaaagccg aggagaaaaa aaaggctgac
     4261 gagttgaaga agaaggcaga agagaagaaa aaagctgatg agcttaaaaa gaaagctgag
     4321 gaaaagaaaa aagccgacga agtcaaaaag gccgaggaaa aaaagaaagc agacgagctt
     4381 aaaaagtccg aagagaagaa gaaggcagac gagctgaaga aatcggagga aaaaaagaag
     4441 gccgacgaat tgaaaaagaa ggcggaggaa aagaagaagg ctgacgaact caagaaaaag
     4501 gctgaagaga agaagaaggc cgacgaactt aaaaagaaag cagaagagaa aaagaaagcc
     4561 gacgagctga aaaaaaaggc tgaagagaaa aaaaaggcgg atgagcttaa gaaaaaagcc
     4621 gaagaaaaaa agaaagccga tgaattgaag aaaaaagcag aggaaaagaa aaaggcggag
     4681 aaccttaaga aagcggaaga aaagaagaaa gcggacgagt tgaaaaagaa agccgaagaa
     4741 aagaaaaagg cagacgaact caaaaagaag gcagaagaaa agaagaaagc agacgaactt
     4801 aagaagaagg ctgaggaaaa aaagaaagct gatgaactta agaaggccga agaaaaaaag
     4861 aaggcggacg aactgaagaa agcagaagag aagaaaaagg cggatgaatt gaaaaaggca
     4921 gaggaaaaga aaaaagctga cgagctgaag aaagccgagg aactgaaaaa agcggaagag
     4981 aaaaagaagg tcgagcagaa gaagcgcgaa gaggaacggc ggaacatggc cctgagaagg
     5041 gccgagatcc tgaaacagat cgagaagaaa cggattgagg aagtcatgaa gctctacgag
     5101 gaagagaaga agatgaaggc cgagcagctc aagaaagagg aagaggaaaa aatcaaggct
     5161 gagcaactta aaaaagaaga ggaagaaaag aaaaaagtcg agcagctgaa gaagaaagaa
     5221 gaagaagaaa agaagaaggc ggaacagctt aagaaagaag aggaagagaa caagatcaaa
     5281 gcggaacaac tgaaaaagaa agaggaagaa gagaagaaaa aggctgagga actgaagaaa
     5341 gaggaagagg aagagaagaa gaaagcagag caattgaaaa aagaagaaga ggagaaaaag
     5401 aaggttgaac aactgaagaa aaaggaggaa gaggaaaaaa agaaagcgga gcagctgaaa
     5461 aaagaggaag aagaaaacaa gatcaaggtc gaacagttga agaaagagga agaagaggaa
     5521 aagaagaagg cagaagaatt gaagaaggag gaagaggaaa agaaaaaggt ccagcagctc
     5581 aaaaaagaag aagagaagaa ggccgaggaa atccggaaag agaaagaggc cgtcatcgag
     5641 gaagaactca aaaaagagga cgaaaagcgg cggatggaag tcgaaaaaaa aattaaggat
     5701 accaaggaca acttcgagaa catccaagag ggcaacaata agaacacgcc ctatatcaac
     5761 aaagagatgt tcgacagcga gatcaaagag gtggtcatca ccaagaacat gcagctgaat
     5821 gaggccgacg ccttcgagaa gcacaacagc gagaacagca agtcctccaa caagaatgcc
     5881 gacttcagca aagagaagga cctgctggaa gatgacatcg agaatatcct ggaaaccgat
     5941 gagaatgaga aaatcaataa ggacgacatc gagcggtcga acaataacat gaaggggaac
     6001 aacaacgaca ttatctacac caagctggac gtggaagagt acaaaaagcg ggatgtgaac
     6061 gaagcccggg aaaagatcat caagatcagc aagaaaaaca tgtgcaacaa tgacttcagc
     6121 tccaagtact gcgactatat gaaggacaat attgccagcg gcacgtgcag caatgaggaa
     6181 cggaagtccc tgtgctgctc catcagcgat ttctgcctga agtactttga ccacaactcg
     6241 aacaagtact acgattgcac caagatcgag ttcgccgacc ctctgtaccg gtgcttcaaa
     6301 aaaaaagagt tcagcaacgg cgcgccgtcg acctccatca cggcctataa gagtgagggg
     6361 gagtcagcgg agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg
     6421 agatggaagg cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac
     6481 caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc
     6541 ccactcaccc ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc
     6601 ctgactctgg acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact
     6661 cagcccgaca gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga
     6721 ctgatcttga agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa
     6781 gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag
     6841 atggactcca agatccaggt tttatccaaa gggttgaatt ccggcggcgg ctccggaggc
     6901 ggtaccggtg gcggcgacct agccccacag atgcttcgag aactccagga gactaatgcg
     6961 gcgctgcaag acgtgagaga gctcttgcga cagcaggtca aggagatcac cttcctgaag
     7021 aatacggtga tggaatgtga cgcctgcggt ctagacagaa acctgcctcc actagcacct
     7081 ctgggcccac acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7141 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7201 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7261 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7321 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7381 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7441 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7501 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7561 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7621 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7681 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7741 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7801 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7861 tcactgatta agcattgggg ccctgactac aaagaccatg acggtgatta taaagatcat
     7921 gacatcgatt acaaggatga cgatgacaag ggccctcacc accaccatca ccactaataa
     7981 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     8041 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     8101 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     8161 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     8221 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     8281 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     8341 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     8401 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     8461 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     8521 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     8581 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     8641 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     8701 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     8761 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     8821 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     8881 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     8941 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     9001 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     9061 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     9121 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     9181 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     9241 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     9301 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     9361 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     9421 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     9481 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     9541 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     9601 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     9661 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     9721 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     9781 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     9841 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     9901 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     9961 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
    10021 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
    10081 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
    10141 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
    10201 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
    10261 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
    10321 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
    10381 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    10441 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    10501 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    10561 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    10621 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    10681 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
    10741 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    10801 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    10861 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    10921 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    10981 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
    11041 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    11101 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    11161 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    11221 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    11281 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    11341 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    11401 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    11461 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    11521 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    11581 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    11641 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    11701 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    11761 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    11821 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    11881 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    11941 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    12001 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    12061 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    12121 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    12181 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    12241 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    12301 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    12361 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    12421 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    12481 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
    12541 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
    12601 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
    12661 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
    12721 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgccat
    12781 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
    12841 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
    12901 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
    12961 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
    13021 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
    13081 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
    13141 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
    13201 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
    13261 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
    13321 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
    13381 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg ggctcgcggt
    13441 tgaggacaaa ctcttcgcgg tctttccagt actcttggat cggaaacccg tcggcctccg
    13501 aacggtactc cgccaccgag ggacctgagc gagtccgcat cgaccggatc ggaaaacct
//
