LOCUS       sequence-223596-        5882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse full-length SynCAM 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Fogel AI, Akins MR, Krupp AJ, Stagi M, Stein V, Biederer T
  TITLE     SynCAMs organize synapses through heterophilic adhesion.
  JOURNAL   J Neurosci. 2007 Nov 14;27(46):12516-30. doi: 
            10.1523/JNEUROSCI.2739-07.2007.
  PUBMED    18003830
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2739-07"; journalName: "J Neurosci"; date: 
            "2007-11-14- 14"; volume: "27"; issue: "46"; pages: "12516-30"
COMMENT     Sequence Label: pCMV5 mSynCAM 2
FEATURES             Location/Qualifiers
     source          1..5882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1526..2148
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(1725..1744)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2172..2192)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2177..2506
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2357..2492
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2544..2563)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2545..2563)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2577..2593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2577..2593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2590..2612)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2601..2617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2625..2631,2632..2649,2650..2655))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2670..2691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2808..2825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2979..3567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3059..3078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3738..4529,4530..4598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4361..4380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4599..4703)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4730..5185
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4817..4836)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5027..5048
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5311..5333
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5325..5342
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5326..5342
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5503..5882
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agaattcgcc cttgaccatg
      241 atttggaaac gcagcgcggt tctccgcttc tacagtgtct gcgggctcct gttacaagcg
      301 gctgcttcaa aaagtaaagt caaaggcagc caagggcagt ttccactaac tcagaatgtc
      361 acggttgttg aaggtggaac tgcaattttg acctgcagag ttgatcaaaa tgataacacc
      421 tccctccagt ggtcaaatcc agctcaacag actctgtact tcgatgacaa gaaagcttta
      481 agggacaatc ggatcgagct ggttcgcgct tcttggcatg agttgagcat cagtgtcagt
      541 gatgtgtctc tctcggatga aggacagtac acctgctcct tatttacaat gcctgtcaaa
      601 acttccaagg cctatctcac tgtcctgggt gttccggaga agcctcagat tagtggattt
      661 tcgtcacccg tcatggaggg agacctgatg cagctgactt gtaagacgtc cggcagtaag
      721 cctgcggctg atataagatg gttcaaaaat gacaaagaaa tcaaagatgt gaagtacttg
      781 aaagaggagg atgctaaccg aaagaccttc accgttagca gcacgctgga tttccgagtg
      841 gaccgtagcg atgatggagt ggcggtcatc tgcagagtag accatgagtc cctcaatgcc
      901 acccctcagg tagccatgca ggtgctagaa attcactata caccatcagt taagattata
      961 ccatcgactc cttttccaca agaagggcag gctttaacgt tgacctgtga atctaaagga
     1021 aaaccactgc cagaacctgt tttgtggacg aaggatggtg ccgaattacc agatcctgat
     1081 cgaatggttg tgagtggtag ggaactcaac attctcttcc tgaacaaaac ggacaatggc
     1141 acttaccgat gtgaagccac taacaccatt ggccaaagca gtgccgagta tgtcctcatt
     1201 gttcacgatc ccaattctct ggctggccag aatggccctg accatgctct catcggagga
     1261 atagtggctg ttgttgtatt tgttacactg tgctccatat tcctgcttgg ccgatatctg
     1321 gcaagacata aaggaacata tctaacaaat gaagctaaag gagccgaaga tgcaccagat
     1381 gccgacacag ccattatcaa tgctgaaggc agccaagtca atgccgaaga gaaaaaagag
     1441 tatttcattt aaaagggcga attcagatct ggtaccacgc gtatcgataa gcttgcatgc
     1501 ctgcaggtcg actctagagg atcccgggtg gcatccctgt gacccctccc cagtgcctct
     1561 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
     1621 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
     1681 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
     1741 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
     1801 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
     1861 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
     1921 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
     1981 cactgctccc ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca
     2041 gacacagcat aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg
     2101 cactgtcctc tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc
     2161 agcttggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     2221 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     2281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     2341 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     2401 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     2461 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgagga
     2521 actgaaaaac cagaaagtta attccctata gtgagtcgta ttaaattcgt aatcatgtca
     2581 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2641 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2701 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2761 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2821 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2881 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2941 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3001 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3061 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3121 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3181 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3241 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3301 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3361 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3421 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3481 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3541 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3601 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3661 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3721 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3781 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3841 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3901 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3961 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4021 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4081 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4141 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4201 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4261 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4321 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4381 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4441 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4501 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4561 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4621 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4681 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     4741 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4801 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4861 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4921 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4981 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5041 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5101 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5161 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
     5221 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     5281 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     5341 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
     5401 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
     5461 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
     5521 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     5581 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     5641 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     5701 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     5761 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     5821 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     5881 tg
//
