LOCUS       sequence-22360-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  TITLE     Birnbaum plasmids
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pShuttle AMPK gamma1
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             114..140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agctctctgg ctaactagag aanccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacgggccc tctagaattc atgtacccat
      121 acgatgttcc agattacgca atggagtcgg ttgctgcaga gagctcgcca gctctagaga
      181 atgaacactt tcaagagacc ccggaatcaa acaatagtgt gtatacttcc ttcatgaagt
      241 ctcatcgctg ctatgaccta attcccacaa gttccaagtt ggtggtattt gacacttcgc
      301 tacaggtaaa gaaagccttt tttgccctgg tgaccaatgg tgttcgtgcc gcccctttgt
      361 gggacagtaa gaagcagagt tttgtgggca tgctgaccat caccgacttc atcaacattt
      421 tgcaccgata ctataagtca gccctggtgc agatctacga actggaggag cacaagatag
      481 agacgtggag agaggtgtac ctgcaggact cctttaagcc acttgtctgc atctctccaa
      541 atgccagctt gtttgatgct gtctcttcat taattcgaaa taagatccac aggctcccag
      601 ttatcgaccc agagtcaggc aacaccttgt acatccttac tcacaagcgg atcctcaagt
      661 tcctcaagtt gtttatcacc nnnntncccc aagccggagt tcatgtctaa gtctcnnnca
      721 ananctgcan annngcaccc tnntgcc
//
