LOCUS       sequence-223604-        9704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constrained LFA-1-mEos3.2 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Moore TI, Aaron J, Chew TL, Springer TA
  TITLE     Measuring Integrin Conformational Change on the Cell Surface with 
            Super-Resolution Microscopy.
  JOURNAL   Cell Rep. 2018 Feb 13;22(7):1903-1912. doi: 
            10.1016/j.celrep.2018.01.062.
  PUBMED    29444440
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.01"; journalName: "Cell Rep"; date: 
            "2018-02-13- 13"; volume: "22"; issue: "7"; pages: "1903-1912"
COMMENT     Sequence Label: aL-mEos3.2
FEATURES             Location/Qualifiers
     source          1..9704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1080..1091
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5126..5143)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5132..5356
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5402..5830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5489..5508)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5699..5720
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5839..5859)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5844..6173
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6024..6159
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6086..6105
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6222..7247
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6222..7247
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7377..7498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7414..7433)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7468..7487
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7547..7563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7547..7563)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7560..7582)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7571..7587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7595..7601,7602..7619,7620..7625))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7640..7661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7778..7795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7949..8537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8029..8048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8708..9499,9500..9568))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9331..9350
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9569..9673)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctgggctag
      901 cgccaccatg aaggattcct gcatcactgt gatggccatg gcgctgctgt ctgggttctt
      961 tttcttcgcg ccggcctcga gctacaacct ggacgtgcgg ggcgcgcgga gcttctcccc
     1021 accgcgcgcc gggaggcact ttggataccg cgtcctgcag gtcggaaacg gggtcatcgt
     1081 gggagctcca ggggagggga acagcacagg aagcctctat cagtgccagt cgggcacagg
     1141 acactgcctg ccagtcaccc tgagaggttc caactatacc tccaagtact tgggaatgac
     1201 cttggcaaca gaccccacag atggaagcat tttggcctgt gaccctgggc tgtctcgaac
     1261 gtgtgaccag aacacctatc tgagtggcct gtgttacctc ttccgccaga atctgcaggg
     1321 tcccatgctg caggggcgcc ctggttttca ggaatgtatc aagggcaacg tagacctggt
     1381 atttctgttt gatggttcga tgagcttgca gccagatgaa tttcagaaaa ttctggactt
     1441 catgaaggat gtgatgaaga aactcagcaa cacttcgtac cagtttgctg ctgttcagtt
     1501 ttccacaagc tacaaaacag aatttgattt ctcagattat gttaaatgga aggaccctga
     1561 tgctctgctg aagcatgtaa agcacatgtt gctgttgacc aatacctttg gtgccatcaa
     1621 ttatgtcgcg acagaggtgt tccgggagga gctgggggcc cggccagatg ccaccaaagt
     1681 gcttatcatc atcacggatg gggaggccac tgacagtggc aacatcgatg cggccaaaga
     1741 catcatccgc tacatcatcg ggattggaaa gcattttcag accaaggaga gtcaggagac
     1801 cctccacaaa tttgcatcaa aacccgcgag cgagtttgtg aaaattctgg acacatttga
     1861 gaagctgaaa gatctattca ctgagctgca gaagaagatc tatgtcattg agggcacaag
     1921 caaacaggac ctgacttcct tcaacatgga gctgtcctcc agcggcatca gtgctgacct
     1981 cagcaggggc catgcagtcg tgggggcagt aggagccaag gactgggctg ggggctttct
     2041 tgacctgaag gcagacctgc aggatgacac atttattggg aatgaaccat tgacaccaga
     2101 agtgagagca ggctatttgg gttacaccgt gacctggctg ccctcccggc aaaagacttc
     2161 gttgctggcc tcgggagccc ctcgatacca gcacatgggc cgagtgctgc tgttccaaga
     2221 gccacaggcc gaggagctgt tcaccggggt gatgaagatc aaactccgta tggaaggcaa
     2281 cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa
     2341 acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct
     2401 gaccactgca ttccattacg gcaacagggt attcgccaaa tatccagaca acatacaaga
     2461 ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga
     2521 cgggggcatt tgcaacgcca gaaacgacat aacaatggaa ggggacactt tctataataa
     2581 agttcgattt tatggtacca actttcccgc caatggtcca gttatgcaga agaagacgct
     2641 gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga cgggtgatat
     2701 tgagatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta
     2761 caaagctaag gagaagggtg tcaagttacc aggcgcccac tttgtggacc actgcattga
     2821 gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctgg
     2881 gatcactcac ggcatggacg agctgtacgc acaacactgg agccaggtcc agacaatcca
     2941 tgggacccag attggctctt atttcggtgg ggagctgtgt ggcgtcgacg tggaccaaga
     3001 tggggagaca gagctgctgc tgattggtgc cccactgttc tatggggagc agagaggagg
     3061 ccgggtgttt atctaccaga gaagacagtt ggggtttgaa gaagtctcag agctgcaggg
     3121 ggaccccggc tacccactcg ggcggtttgg agaagccatc actgctctga cagacatcaa
     3181 cggcgatggg ctggtagacg tggctgtggg ggcccctctg gaggagcagg gggctgtgta
     3241 catcttcaat gggaggcacg gggggcttag tccccagcca agtcagcgga tagaagggac
     3301 ccaagtgctc tcaggaattc agtggtttgg acgctccatc catggggtga aggaccttga
     3361 aggggatggc ttggcagatg tggctgtggg ggctgagagc cagatgatcg tgctgagctc
     3421 ccggcccgtg gtggatatgg tcaccctgat gtccttctct ccagctgaga tcccagtgca
     3481 tgaagtggag tgctcctatt caaccagtaa caagatgaaa gaaggagtta atatcacaat
     3541 ctgtttccag atcaagtctc tcatccccca gttccaaggc cgcctggttg ccaatctcac
     3601 ttacactctg cagctggatg gccaccggac cagaagacgg gggttgttcc caggagggag
     3661 acatgaactc agaaggaata tagctgtcac caccagcatg tcatgcactg acttctcatt
     3721 tcatttcccg gtatgtgttc aagacctcat ctcccccatc aatgtttccc tgaatttctc
     3781 tctttgggag gaggaaggga caccgaggga ccaaagggcg cagggcaagg acataccgcc
     3841 catcctgaga ccctccctgc actcggaaac ctgggagatc ccttttgaga agaactgtgg
     3901 ggaggacaag aagtgtgagg caaacttgag agtgtccttc tctcctgcaa gatccagagc
     3961 cctgcgtcta actgcttttg ccagcctctc tgtggagctg agcctgagta acttggaaga
     4021 agatgcttac tgggtccagc tggacctgca cttccccccg ggactctcct tccgcaaggt
     4081 ggagatgctg aagccccata gccagatacc tgtgagctgc gaggagcttc ctgaagagtc
     4141 caggcttctg tccagggcat tatcttgcaa tgtgagctct cccatcttca aagcaggcca
     4201 ctcggttgct ctgcagatga tgtttaatac actggtaaac agctcctggg gggactcggt
     4261 tgaattgcac gccaatgtga cctgtaacaa tgaggactca gacctcctgg aggacaactc
     4321 agccactacc atcatcccca tcctgtaccc catcaacatc ctcatccagg accaagaaga
     4381 ctccacactc tatgtcagtt tcacccccaa aggccccaag atccaccaag tcaagcacat
     4441 gtaccaggtg aggatccagc cttccatcca cgaccacaac atacccaccc tggaggctgt
     4501 ggttggggtg ccacagcctc ccagcgaggg gcccatcaca caccagtgga gcgtgcagat
     4561 ggagcctccc gtgccctgcc actatgagga tctggagagg ctcccggatg cagctgagcc
     4621 ttgtctcccc ggagccctgt tccgctgccc tgttgtcttc aggcaggaga tcctcgtcca
     4681 agtgatcggg actctggagc tggtgggaga gatcgaggcc tcttccatgt tcagcctctg
     4741 cagctccctc tccatctcct tcaacagcag caagcatttc cacctctatg gcagcaacgc
     4801 ctccctggcc caggttgtca tgaaggttga cgtggtgtat gagaagcaga tgctctacct
     4861 ctacgtgctg agcggcatcg gggggctgct gctgctgctg ctcattttca tagtgctgta
     4921 caaggttggt ttcttcaaac ggaacctgaa ggagaagatg gaggctggca gaggtgtccc
     4981 gaatggaatc cctgcagaag actctgagca gctggcatct gggcaagagg ctggggatcc
     5041 cggctgcctg aagcccctcc atgagaagga ctctgagagt ggtggtggca aggactgata
     5101 acttaaggtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5161 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5221 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5281 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5341 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     5401 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5461 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5521 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5581 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5641 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5701 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5761 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5821 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5881 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5941 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6001 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6061 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     6121 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     6181 cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag cctgaactca
     6241 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     6301 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     6361 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     6421 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     6481 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     6541 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     6601 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     6661 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     6721 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     6781 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     6841 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     6901 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     6961 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     7021 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     7081 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     7141 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     7201 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtgctacgag
     7261 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     7321 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     7381 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7441 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7501 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7561 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7621 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7681 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7741 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7801 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7861 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7921 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7981 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8041 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8101 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8161 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8221 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8281 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8341 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8401 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8461 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8521 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8581 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8641 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8701 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8761 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8821 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8881 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8941 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9001 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9061 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9121 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9181 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9241 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9301 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9361 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9421 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9481 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9541 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9601 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9661 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
