LOCUS       sequence-223607-        8670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human alpha5 ectodomain with secretion peptide, purification
            tags and C-terminal clasp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Li J, Su Y, Xia W, Qin Y, Humphries MJ, Vestweber D, Cabanas C, Lu 
            C, Springer TA
  TITLE     Conformational equilibria and intrinsic affinities define integrin 
            activation.
  JOURNAL   EMBO J. 2017 Mar 1;36(5):629-645. doi: 10.15252/embj.201695803. Epub
            2017 Jan 25.
  PUBMED    28122868
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201695803"; journalName: "EMBO J"; date: "2017-03-1- 
            1"; volume: "36"; issue: "5"; pages: "629-645"
COMMENT     Sequence Label: a5-ecto-pcDNA31hygro
FEATURES             Location/Qualifiers
     source          1..8670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3921..3941
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4005..4016
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4041..4064
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(4092..4109)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4098..4322
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4368..4796
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4455..4474)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4665..4686
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4805..4825)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4810..5139
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4990..5125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5052..5071
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5188..6213
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5188..6213
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6343..6464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6380..6399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6434..6453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6513..6529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6513..6529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6526..6548)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6537..6553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6561..6567,6568..6585,6586..6591))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6606..6627
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6744..6761)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6915..7503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6995..7014)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7674..8465,8466..8534))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8297..8316
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8535..8639)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga gctatgggga gccggacgcc agagtcccct ctccacgccg
      961 tgcagctgcg ctggggcccc cggcgccgac ccccgctgct gccgctgctg ttgctgctgc
     1021 tgccgccgcc acccagggtc gggggcttca acttagacgc ggaggcccca gcagtactct
     1081 cggggccccc gggctccttc ttcggattct cagtggagtt ttaccggccg ggaacagacg
     1141 gggtcagcgt gctggtggga gcacccaagg ctaataccag ccagccagga gtgctgcagg
     1201 gtggtgctgt ctacctctgt ccttggggtg ccagccccac acagtgcacc cccattgaat
     1261 ttgacagcaa aggctctcgg ctcctggagt cctcactgtc cagctcagag ggagaggagc
     1321 ctgtggagta caagtccttg cagtggttcg gggcaacagt tcgagcccat ggctcctcca
     1381 tcttggcatg cgctccactg tacagctggc gcacagagaa ggagccactg agcgaccccg
     1441 tgggcacctg ctacctctcc acagataact tcacccgaat tctggagtat gcaccctgcc
     1501 gctcagattt cagctgggca gcaggacagg gttactgcca aggaggcttc agtgccgagt
     1561 tcaccaagac tggccgtgtg gttttaggtg gaccaggaag ctatttctgg caaggccaga
     1621 tcctgtctgc cactcaggag cagattgcag aatcttatta ccccgagtac ctgatcaacc
     1681 tggttcaggg gcagctgcag actcgccagg ccagttccat ctatgatgac agctacctag
     1741 gatactctgt ggctgttggt gaattcagtg gtgatgacac agaagacttt gttgctggtg
     1801 tgcccaaagg gaacctcact tacggctatg tcaccatcct taatggctca gacattcgat
     1861 ccctctacaa cttctcaggg gaacagatgg cctcctactt tggctatgca gtggccgcca
     1921 cagacgtcaa tggggacggg ctggatgact tgctggtggg ggcacccctg ctcatggatc
     1981 ggacccctga cgggcggcct caggaggtgg gcagggtcta cgtctacctg cagcacccag
     2041 ccggcataga gcccacgccc acccttaccc tcactggcca tgatgagttt ggccgatttg
     2101 gcagctcctt gacccccctg ggggacctgg accaggatgg ctacaatgat gtggccatcg
     2161 gggctccctt tggtggggag acccagcagg gagtagtgtt tgtatttcct gggggcccag
     2221 gagggctggg ctctaagcct tcccaggttc tgcagcccct gtgggcagcc agccacaccc
     2281 cagacttctt tggctctgcc cttcgaggag gccgagacct ggatggcaat ggatatcctg
     2341 atctgattgt ggggtccttt ggtgtggaca aggctgtggt atacaggggc cgccccatcg
     2401 tgtccgctag tgcctccctc accatcttcc ccgccatgtt caacccagag gagcggagct
     2461 gcagcttaga ggggaaccct gtggcctgca tcaaccttag cttctgcctc aatgcttctg
     2521 gaaaacacgt tgctgactcc attggtttca cagtggaact tcagctggac tggcagaagc
     2581 agaagggagg ggtacggcgg gcactgttcc tggcctccag gcaggcaacc ctgacccaga
     2641 ccctgctcat ccagaatggg gctcgagagg attgcagaga gatgaagatc tacctcagga
     2701 acgagtcaga atttcgagac aaactctcgc cgattcacat cgctctcaac ttctccttgg
     2761 acccccaagc cccagtggac agccacggcc tcaggccagc cctacattat cagagcaaga
     2821 gccggataga ggacaaggct cagatcttgc tggactgtgg agaagacaac atctgtgtgc
     2881 ctgacctgca gctggaagtg tttggggagc agaaccatgt gtacctgggt gacaagaatg
     2941 ccctgaacct cactttccat gcccagaatg tgggtgaggg tggcgcctat gaggctgagc
     3001 ttcgggtcac cgcccctcca gaggctgagt actcaggact cgtcagacac ccagggaact
     3061 tctccagcct gagctgtgac tactttgccg tgaaccagag ccgcctgctg gtgtgtgacc
     3121 tgggcaaccc catgaaggca ggagccagtc tgtggggtgg ccttcggttt acagtccctc
     3181 atctccggga cactaagaaa accatccagt ttgacttcca gatcctcagc aagaatctca
     3241 acaactcgca aagcgacgtg gtttcctttc ggctctccgt ggaggctcag gcccaggtca
     3301 ccctgaacgg tgtctccaag cctgaggcag tgctattccc agtaagcgac tggcatcccc
     3361 gagaccagcc tcagaaggag gaggacctgg gacctgctgt ccaccatgtc tatgagctca
     3421 tcaaccaagg ccccagctcc attagccagg gtgtgctgga actcagctgt ccccaggctc
     3481 tggaaggtca gcagctccta tatgtgacca gagttacggg actcaactgc accaccaatc
     3541 accccattaa cccaaagggc ctggagttgg atcccgaggg ttccctgcac caccagcaaa
     3601 aacgggaagc tccaagccgc agctctgctt cctcgggacc tcagatcctg aaatgcccgg
     3661 aggctgagtg tttcaggctg cgctgtgagc tcgggcccct gcaccaacaa gagagccaaa
     3721 gtctgcagtt gcatttccga gtctgggcca agactttctt gcagcgggag caccagccat
     3781 ttagcctgca gtgtgaggct gtgtacaaag ccctgaagat gccctaccga atcctgcctc
     3841 ggcagctgcc ccaaaaagag cgtcaggtgg ccacagctgt gcaatggacc aaggcagaag
     3901 gcagctatac cggtggactg gagaatcttt atttccaagg tggagaaaat gctcagtgcg
     3961 aaaaagagct ccaggcactt gagaaggaaa atgcacagct ggaatgggaa ttacaagcac
     4021 tagagaagga gctagctcaa tggagccacc cccagttcga aaagtaagct taagtttaaa
     4081 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4141 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4201 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4261 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4321 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4381 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4441 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4501 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4561 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4621 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4681 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4741 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4801 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4861 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4921 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4981 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5041 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     5101 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     5161 tccattttcg gatctgatca gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc
     5221 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     5281 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     5341 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     5401 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     5461 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     5521 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     5581 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     5641 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     5701 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     5761 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     5821 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     5881 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     5941 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     6001 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     6061 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     6121 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     6181 cgccccagca ctcgtccgag ggcaaaggaa tagcacgtgc tacgagattt cgattccacc
     6241 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6301 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6481 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6541 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6601 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6661 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6721 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6781 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6841 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6901 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6961 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7021 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7081 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7141 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7201 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7261 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7321 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7381 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7441 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7501 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7561 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7621 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7681 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7741 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7801 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7861 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7921 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7981 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8041 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8101 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8161 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8221 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8281 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8341 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8401 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8461 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8521 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8581 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8641 acatttcccc gaaaagtgcc acctgacgtc
//
