LOCUS       sequence-22361-e         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  TITLE     Birnbaum plasmids
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pShuttle AMPK gamma1 H150R
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacgggccct ctagaattca tgtacccata
      121 cgatgttcca gattacgcaa tggagtcggt tgctgcagag agctcgccag ctctagagaa
      181 tgaacacttt caagagaccc cggaatcaaa caatagtgtg tatacttcct tcatgaagtc
      241 tcatcgctgc tatgacctaa ttcccacaag ttccaagttg gtggtatttg acacttcgct
      301 acaggtaaag aaagcctttt ttgccctggt gaccaatggt gttcgtgccg cccctttgtg
      361 ggacagtaag aagcagagtt ttgtgggcat gctgaccatc accgacttca tcaacatttt
      421 gcaccgatac tataagtcag ccctggtgca gatctacgaa ctggaggagc acaagataga
      481 gacgtggaga gaggtgtacc tgcaggactc ctttaagcca cttgtctgca tctctccaaa
      541 tgccagcttg tttgatgctg tctcttcatt aattcgaaat aagatccgca ggcc
//
