LOCUS       sequence-22362-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  TITLE     Birnbaum plasmids
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pShuttle AMPK gamma1 H150R
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    392..616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     605..622
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cctctgattg agcgtgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agacccataa
      301 tacccataat gccatttcat tacctctttc tccgcacccg acatagatga attcagatct
      361 gcagatctgc tggttctttc cgcctcagaa gccatagagc ccaccgcatc cccagcatgc
      421 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
      481 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
      541 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
      601 caactagaag gcacagtcga ggctgatcag cggtttaaac ttaagcttgg taccgagctc
      661 ggatcatcca gcacagtggc ggccgccctt tttttttttt ttttaccatg atgaaacctt
      721 gtcatttaat tagattcgcc acaaaaagca ttaatcttgg
//
