LOCUS       sequence-223624-        4459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  firefly luciferase (Fluc) reporter containing the 5'-UTR of GNB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ku YC, Lai MH, Lo CC, Cheng YC, Qiu JT, Tarn WY, Lai MC
  TITLE     DDX3 Participates in Translational Control of Inflammation Induced 
            by Infections and Injuries.
  JOURNAL   Mol Cell Biol. 2018 Dec 11;39(1). pii: MCB.00285-18. doi: 
            10.1128/MCB.00285-18. Print 2019 Jan 1.
  PUBMED    30373933
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2018-12-11"; volume: "39(1). pii: MCB.00285-18. doi";
            pages: " 10.1128/MCB.00285-18. Print 2019 Jan 1"
COMMENT     Sequence Label: pFL-SV40-GNB2 5'-UTR
FEATURES             Location/Qualifiers
     source          1..4459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      725..734
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             731..2383
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(797..815)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2414..2535
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2425..2444)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2479..2498
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2668..2772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2773..2841,2842..3633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2991..3010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3804..4392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4293..4312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttactgg cgggcgggat ccctccgctc tggggaggca gcgctggcgg cggggctggg
      481 gccactgagg aaatccatcc gcgccgccgc cgccgccgcc gccgccgccg ccgcctccgc
      541 cgcggaggaa gacagcgccg cccgcgcacc gccagcgacc cccgccgcag agtcccaccg
      601 ccacaggcct cgggccagcg gccaggagct gcctccccca gcccccgtcc cgcggccccc
      661 agccgccccc aaccctgccc cacgggcccg gcgccaagct tggcattccg gtactgttgg
      721 taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct
      781 ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
      841 tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt
      901 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
      961 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
     1021 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag
     1081 tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt
     1141 gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga
     1201 ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa
     1261 tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa
     1321 ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt
     1381 gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat
     1441 tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat
     1501 atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct
     1561 tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa
     1621 aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc
     1681 tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag
     1741 gcaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga
     1801 taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga
     1861 taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat
     1921 tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg
     1981 gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg
     2041 cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat
     2101 cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc
     2161 cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga
     2221 gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt
     2281 gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga
     2341 gatcctcata aaggccaaga agggcggaaa gatcgccgtg taattctaga gcggccgctt
     2401 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     2461 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     2521 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     2581 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     2641 ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2701 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2761 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2821 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2881 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2941 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3001 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3061 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3121 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3181 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3241 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3301 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3361 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3421 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3481 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3541 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3601 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3661 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3721 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3781 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3841 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3901 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3961 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4021 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4081 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4141 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4201 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4261 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4321 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4381 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4441 tttgctcaca tggctcgac
//
