LOCUS       sequence-223625-         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  firefly luciferase (Fluc) reporter containing the 5'-UTR of GNB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Ku YC, Lai MH, Lo CC, Cheng YC, Qiu JT, Tarn WY, Lai MC
  TITLE     DDX3 Participates in Translational Control of Inflammation Induced 
            by Infections and Injuries.
  JOURNAL   Mol Cell Biol. 2018 Dec 11;39(1). pii: MCB.00285-18. doi: 
            10.1128/MCB.00285-18. Print 2019 Jan 1.
  PUBMED    30373933
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2018-12-11"; volume: "39(1). pii: MCB.00285-18. doi";
            pages: " 10.1128/MCB.00285-18. Print 2019 Jan 1"
COMMENT     Sequence Label: pFL-SV40-GNB2 5'-UTR
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      286..295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(358..376)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tccctccgct ctggggaggc agcgctggcg gcggggctgg ggccactgag gaaatccatc
       61 cgcgccgccg ccgccgccgc cgccgccgcc gccgcctccg ccgcggagga agacagcgcc
      121 gcccgcgcac cgccagcgac ccccgccgca gagtcccacc gccacaggcc tcgggccagc
      181 ggccaggagc tgcctccccc agcccccgtc ccgcggcccc cagccgcccc caaccctgcc
      241 ccacgggccc ggcgccaagc ttggcattcc ggtactgttg gtaaagccac catggaagac
      301 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga
      361 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
      421 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg
      481 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
      541 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
      601 cccgcgaa
//
