LOCUS       sequence-223628-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50004
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..768
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(203..221)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(805..823)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        891..995
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcaaaccgc ctctccccgc gcgttggccg attcattaat cactgtggtc tcattcgatg
       61 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
      121 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      181 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
      241 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
      301 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      361 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
      421 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
      481 cagaagaaaa ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
      541 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
      601 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
      661 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
      721 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtgagc tttgagacca
      781 cgtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      841 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      961 gataaatgct tcaataatat tgaaaaagga agagta
//
