LOCUS       sequence-22364-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  TITLE     Birnbaum plasmids
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3 HA PKB AAA
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             157..183
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             163..189
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
      121 ctggaattct gcagataatt cggctagacc accatgtacc catacgatgt tccagattac
      181 gcaagcttgg gtggtcccat ggacgacgtg gccatcgtga aggagggctg gctgcacaag
      241 cgaggtgagt acatcaagac gtggcggccg cgttacttcc tcctgaagaa cgatggcacg
      301 ttcattggct acaaggagcg gccgcaggac ctggagcagc gtgagtcgcc cctcaacaac
      361 ttctccgtgg cccaatgcca gctgatgaag acggagcggc cgcggcccaa caccttcatc
      421 atccgctgcc tgcagtggac cacggtcatc gagcgcacgt tccacgtgga gacgcccgag
      481 gaagcgggag gagtggacca ccgccatcca gacggtggcc gacgggctca agaggcagga
      541 ggaggagacg atggacttcc ggtcgggctc acccggcgag aactcggggg ccgaggagat
      601 ggaggtgtcg ctggccaagc ccaagcaccg cgtgaccatg aatgagtttg agtacctgaa
      661 gctgctgggc aaaggcacct ttcgggaagg tgatcctggt ggaaggagaa ggccaca
//
