LOCUS       sequence-223645-        9666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Roussel MF, Dull TJ, Rettenmier CW, Ralph P, Ullrich A, Sherr CJ
  TITLE     Transforming potential of the c-fms proto-oncogene (CSF-1 receptor).
  JOURNAL   Nature. 1987 Feb 5-11;325(6104):549-52.
  PUBMED    3027579
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1987 Feb 5-11"; volume: "325(6104)"; pages: "549-52"
COMMENT     Sequence Label: MSCV-human CSF-1Rwt-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    170..511
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             578..994
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     924..946
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     962..978
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    join(4265..4839,4840..4842,4843..4851)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4432..4449)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4659..4678
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4852..4854,4855..4857,4858..5571)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4852..4854,4855..4857,4858..5568)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4897..4918)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5158..5177)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5505..5526
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5650..6164
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6326..6342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6350..6356,6357..6374,6375..6380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6395..6416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6533..6550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6704..7292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6784..6803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7463..8254,8255..8323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8086..8105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8324..8428)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8496..8514
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8552..8574)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8674..8693
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8887..8909
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9037..9056
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9256..9666,1..106)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tattcccaat aaagcctctt gctgtttgca tccgaatcgt ggactcgctg atccttggga
       61 gggtctcctc agattgattg actgcccacc tcgggggtct ttcatttgga ggttccaccg
      121 agatttggag acccctgccc agggaccacc gacccccccg ccgggaggta agctggccag
      181 cggtcgtttc gtgtctgtct ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg
      241 cctgcgtctg tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga
      301 cgagttctga acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt
      361 ttgtggcccg acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt
      421 tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt
      481 ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct
      541 ctgtctgact gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt
      601 aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga
      661 tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg
      721 atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt
      781 ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt
      841 ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct
      901 tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc
      961 cctttatcca gccctcactc cttctctagg cgccggaatt cgttaacctc gagagatccg
     1021 cggccgctga tccgaattcg catggtcgac taccagagga gaggaacgtg tgtccagtgt
     1081 cccgatccct gcggagctag tagctgagag ctctgtgccc tgggcacctt gcagccctgc
     1141 acctgcctgc cacttcccca ccgaggccat gggcccagga gttctgctgc tcctgctggt
     1201 ggccacagct tggcatggtc agggaatccc agtgatagag cccagtgtcc ccgagctggt
     1261 cgtgaagcca ggagcaacgg tgaccttgcg atgtgtgggc aatggcagcg tggaatggga
     1321 tggcccccca tcacctcact ggaccctgta ctctgatggc tccagcagca tcctcagcac
     1381 caacaacgct accttccaaa acacggggac ctatcgctgc actgagcctg gagaccccct
     1441 gggaggcagc gccgccatcc acctctatgt caaagaccct gcccggccct ggaacgtgct
     1501 agcacaggag gtggtcgtgt tcgaggacca ggacgcacta ctgccctgtc tgctcacaga
     1561 cccggtgctg gaagcaggcg tctcgctggt gcgtgtgcgt ggccggcccc tcatgcgcca
     1621 caccaactac tccttctcgc cctggcatgg cttcaccatc cacagggcca agttcattca
     1681 gagccaggac tatcaatgca gtgccctgat gggtggcagg aaggtgatgt ccatcagcat
     1741 ccggctgaaa gtgcagaaag tcatcccagg gcccccagcc ttgacactgg tgcctgcaga
     1801 gctggtgcgg attcgagggg aggctgccca gatcgtgtgc tcagccagca gcgttgatgt
     1861 taactttgat gtcttcctcc aacacaacaa cactaagctc gcaatccctc aacaatctga
     1921 ctttcataat aaccgttacc aaaaagtcct gaccctcaac ctcgatcaag tagatttcca
     1981 acatgccggc aactactcct gcgtggccag caacgtgcag ggcaagcact ccacctccat
     2041 gttcttccgg gtggtagaga gtgcctactt gaacttgagc tctgagcaga acctcatcca
     2101 ggaggtgacc gtgggggagg ggctcaacct caaagtcatg gtggaggcct acccaggcct
     2161 gcaaggtttt aactggacct acctgggacc cttttctgac caccagcctg agcccaagct
     2221 tgctaatgct accaccaagg acacatacag gcacaccttc accctctctc tgccccgcct
     2281 gaagccctct gaggctggcc gctactcctt cctggccaga aacccaggag gctggagagc
     2341 tctgacgttt gagctcaccc ttcgataccc cccagaggta agcgtcatat ggacattcat
     2401 caacggctct ggcacccttt tgtgtgctgc ctctgggtac ccccagccca acgtgacatg
     2461 gctgcagtgc agtggccaca ctgataggtg tgatgaggcc caagtgctgc aggtctggga
     2521 tgacccatac cctgaggtcc tgagccagga gcccttccac aaggtgacgg tgcagagcct
     2581 gctgactgtt gagaccttag agcacaacca aacctacgag tgcagggccc acaacagcgt
     2641 ggggagtggc tcctgggcct tcatacccat ctctgcagga gcccacacgc atcccccgga
     2701 tgagttcctc ttcacaccag tggtggtcgc ctgcatgtcc atcatggcct tgctgctgct
     2761 gctgctcctg ctgctattgt acaagtataa gcagaagccc aagtaccagg tccgctggaa
     2821 gatcatcgag agctatgagg gcaacagtta tactttcatc gaccccacgc agctgcctta
     2881 caacgagaag tgggagttcc cccggaacaa cctgcagttt ggtaagaccc tcggagctgg
     2941 agcctttggg aaggtggtgg aggccacggc ctttggtctg ggcaaggagg atgctgtcct
     3001 gaaggtggct gtgaagatgc tgaagtccac ggcccatgct gatgagaagg aggccctcat
     3061 gtccgagctg aagatcatga gccacctggg ccagcacgag aacatcgtca accttctggg
     3121 agcctgtacc catggaggcc ctgtactggt catcacggag tactgttgct atggcgacct
     3181 gctcaacttt ctgcgaagga aggctgaggc catgctggga cccagcctga gccccggcca
     3241 ggaccccgag ggaggcgtcg actataagaa catccacctc gagaagaaat atgtccgcag
     3301 ggacagtggc ttctccagcc agggtgtgga cacctatgtg gagatgaggc ctgtctccac
     3361 ttcttcaaat gactccttct ctgagcaaga cctggacaag gaggatggac ggcccctgga
     3421 gctccgggac ctgcttcact tctccagcca agtagcccag ggcatggcct tcctcgcttc
     3481 caagaattgc atccaccggg acgtggcagc gcgtaacgtg ctgttgacca atggtcatgt
     3541 ggccaagatt ggggacttcg ggctggctag ggacatcatg aatgactcca actacattgt
     3601 caagggcaat gcccgcctgc ctgtgaagtg gatggcccca gagagcatct ttgactgtgt
     3661 ctacacggtt cagagcgacg tctggtccta tggcatcctc ctctgggaga tcttctcact
     3721 tgggctgaat ccctaccctg gcatcctggt gaacagcaag ttctataaac tggtgaagga
     3781 tggataccaa atggcccagc ctgcatttgc cccaaagaat atatacagca tcatgcaggc
     3841 ctgctgggcc ttggagccca cccacagacc caccttccag cagatctgct ccttccttca
     3901 ggagcaggcc caagaggaca ggagagagcg ggactatacc aatctgccga gcagcagcag
     3961 aagcggtggc agcggcagca gcagcagtga gctggaggag gagagctcta gtgagcacct
     4021 gacctgctgc gagcaagggg atatcgccca gcccttgctg cagcccaaca actatcagtt
     4081 ctgctgagga gttgacgaca gggagtacca ctctcccctc ctccaaactt caactcctcc
     4141 atggatgggg cgacacgggg agaacataca aactctgcct tcggtcattt cactcaacag
     4201 ctcggcccag ctctgaaact tgggaaggtg agggattcag gggaggtcag aggatcagcg
     4261 gccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4321 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4381 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4441 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4501 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4561 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4621 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4681 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4741 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     4801 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc
     4861 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4921 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     4981 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     5041 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     5101 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     5161 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     5221 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     5281 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     5341 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     5401 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     5461 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5521 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta actcgaggga
     5581 tccgtcgacc tgcagccaag cttatcgata aaataaaaga ttttatttag tctccagaaa
     5641 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     5701 tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag
     5761 agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     5821 agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca
     5881 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     5941 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     6001 cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt
     6061 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     6121 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatgggtaa cagtttcttg
     6181 aagttggaga acaacattct gagggtagga gtcgaatatt aagtaatcct gactcaatta
     6241 gccactgttt tgaatccaca tactccaata ctcctgaaat agttcattat ggacagcgca
     6301 gaaagagctg gggagaattg tgaaattgtt atccgctcac aattccacac aacatacgag
     6361 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6421 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6481 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6541 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6601 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6661 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6721 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6781 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6841 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6901 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6961 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7021 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7081 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7141 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7201 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7261 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7321 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7381 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7441 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7501 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7561 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7621 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7681 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7741 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7801 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7861 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7921 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7981 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8041 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8101 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8161 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8221 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8281 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8341 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8401 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8461 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8521 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8581 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8641 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8701 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     8761 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     8821 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     8881 ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat gcaaggagat
     8941 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     9001 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     9061 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggcgatt
     9121 agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata
     9181 tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct
     9241 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     9301 gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag atcaaggtta
     9361 ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     9421 ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag
     9481 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     9541 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     9601 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc gtcgcccggg
     9661 tacccg
//
