LOCUS       sequence-223662-        5933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Wang X, Srivastava Y, Jankowski A, Malik V, Wei Y, Del Rosario RC, 
            Cojocaru V, Prabhakar S, Jauch R
  TITLE     DNA-mediated dimerization on a compact sequence signature controls 
            enhancer engagement and regulation by FOXA1.
  JOURNAL   Nucleic Acids Res. 2018 Jun 20;46(11):5470-5486. doi: 
            10.1093/nar/gky259.
  PUBMED    29669022
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-06-20- 20"; volume: "46"; issue: 
            "11"; pages: "5470-5486"
COMMENT     Sequence Label: PVT1-Tol2
FEATURES             Location/Qualifiers
     source          1..5933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(483..670,671..682)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(694..713)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(695..713)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(720..737)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(720..736)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(729..751)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      877..1332
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(964..983)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1174..1195
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1410..1514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1515..1583,1584..2375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1733..1752)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2546..3134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3035..3054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3288..3305
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3422..3443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3458..3463,3464..3481,3482..3488)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3496..3512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3501..3523
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3520..3536
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3520..3536
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3554..3572
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3554..3571
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(3623..3634,3635..3797)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             join(4890..4892,4893..4895,4896..5609)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4890..4892,4893..4895,4896..5606)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4935..4956)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5196..5215)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5543..5564
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5732..5853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5769..5788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5823..5842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aaaaaactat cagtaaaaca aaatacttaa tgtacttacc tcaatatgct tccttaggtt
       61 tgatggcgaa cttttgaagg ccgatatttc tttattaagc gggagacaga ggacacattt
      121 catcttgaat gaatctttat ttacaccact taaagaaaag aattcgcgaa gatacggcca
      181 cgggtgctct tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc
      241 acatacttcc tccattttct tccactgatt gactgttata atttccctaa tttccaggtc
      301 aaggtgctgt gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa
      361 catgtctgac caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt
      421 taaattaaac tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa
      481 tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa
      541 agtacttttt tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg
      601 tactcaagta aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt
      661 actcaagtac tttacacctc tgggcccaat tcgccctata gtgagtcgta ttacaattca
      721 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      781 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      841 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
      901 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      961 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     1021 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     1081 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     1141 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     1201 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     1261 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     1321 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     1381 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1441 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1501 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1561 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1621 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1681 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1741 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1801 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1861 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1921 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1981 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2041 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2101 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2161 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2221 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2281 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2341 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2401 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2461 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2521 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2581 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2641 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2701 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2761 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2821 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2881 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2941 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3001 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3061 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3121 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3181 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3241 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3301 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3361 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3421 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     3481 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     3541 attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc caacgcgttg
     3601 ggagctctcc catatggtcg agcagaggtg taaaaagtac tcaaaaattt tactcaagtg
     3661 aaagtacaag tacttaggga aaattttact caattaaaag taaaagtatc tggctagaat
     3721 cttacttgag taaaagtaaa aaagtactcc attaaaattg tacttgagta ttaaggaagt
     3781 aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta agcttttaat ctcaaaaaac
     3841 attaaatgaa atgcatacaa ggttttatcc tgctttagaa ctgtttgtat ttaattatca
     3901 aactataaga cagacaatct aatgccagta cacgctactc aaagttgtaa aacctcagat
     3961 ttaacttcag tagaagctga ttctcaaaat tgttagtgtc aagcctagct cttttggggc
     4021 tgaaaagcaa tcctgcagtc gacgtcggcc atagcggccg cggaactggt ggacattggg
     4081 tctttttaga tgtcattact taagatgata tcatattgga ttagggtggg ggctaaagcc
     4141 aatgacccat gtcctcagag gagaagagag ggacatagga aagaaggatt tgtagagata
     4201 cagggagaag gccatgtgac cacagaggca gagattagag tgacacagct acaagccaag
     4261 gagctccaag gattgcagga agccaccaga tgccagaaag aggcaaggaa ggatctttca
     4321 tggagccttc agagggagca cagtcccgct gacaccttga tcttggacat ctagtcccca
     4381 gaactgtgag agagtgcatc tgctgatgta agccacccag tttgtagcaa tttgttatgg
     4441 ctgccctcag aaactaagac agtagccaac acagaaccaa ataccctttg gggattgagg
     4501 gaagtggagc gggagaatga gcggcactcc ctccctttac ataggactac ttggtaaata
     4561 tttactgaat gaaggaaagc tgaagtatct gtagaactgg ccatttcatt ctgcatttag
     4621 agctctggga gctcttgaaa tgtctagaca aacatcctct tcagctagag gaagctcctt
     4681 ctcccactta ctagctatga gatcttgggc agatggctta acatttctgg gcctcagtga
     4741 ccttacttgc aaagttgtct gggtcaaggc aatgatcgcg gatccgcgga tcccccagtg
     4801 acgtaggaag tccatccatt cacagcgctt ctataaaggc gccagctgag gcgcctacta
     4861 ctccaaccgc gactgcagcg agcaactcca tggtgagcaa gggcgaggag ctgttcaccg
     4921 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     4981 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     5041 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     5101 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     5161 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     5221 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     5281 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     5341 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     5401 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     5461 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     5521 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     5581 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
     5641 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5701 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     5761 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5821 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatccta
     5881 ctacacatat aaaaggcgcg ccatcaacgt caaaacatgc acacccaaaa aaa
//
