LOCUS       sequence-223664-       14607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU11.1-2.2
FEATURES             Location/Qualifiers
     source          1..14607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1..17)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      1531..1830
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4486..4706
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4707..5510
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(10757..10777)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10757..10775)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(11114..11702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11194..11213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11873..12664,12665..12733))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12496..12515
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12734..12838)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12865..14207
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(13828..13875)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     14248..14266
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatccactag ttctagatta attaatatct aaaaattgcc ttatgatccg tctctccggt
       61 tacagcctgt gtaactgatt aatcctgcct ttctaatcac cattctaatg ttttaattaa
      121 gggattttgt cttcattaac ggctttcgct cataaaaatg ttatgacgtt ttgcccgcag
      181 gcgggaaacc atccacttca cgagactgat ctcctctgcc ggaacaccgg gcatctccaa
      241 cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaaa
      301 aaaggcagag gagagcatag aaatggggtt cactttttgg taaagctata gcatgcctat
      361 cacatataaa tagagtgcca gtagcgactt ttttcacact cgaaatactc ttactactgc
      421 tctcttgttg tttttatcac ttcttgtttc ttcttggtaa atagaatatc aagctacaaa
      481 aagcatacaa tcaactatca actattaact atatcgtaat acacaatgtt ttcagaatcc
      541 catctcaaga tgtctcgtac cttcacccag ttctgtttcc cttctgtctc gccatcctca
      601 ttggtaattg aagtcgactt cttgtcgtct ttcgctttgc cagtgctacg tttcattgtc
      661 aaactccctt catacgcctt gtaccatcta tggacgctat ttttattcac gaagtcagac
      721 attaagacaa ctgtaatacc tataacttct ctggccgtcg cgtccgctcc acttggcgat
      781 atctcacatc ttccacatgt tatattctgg atttggtttc atgtcctaca gttcgacgtc
      841 tcaaaccaga cgctgaaacc agaagaagac gagtacaaca agcgagaccg tcctctgccc
      901 gcaaagcgca tcacttggcg caatgcattg attttgcgtt gggttctcgt cccggcgtgt
      961 tggatactct catcctttta cagtgttgaa actgtgtatg cgagtatcgc actgtgcatt
     1021 ctgacatatg tatacgacga aatgggttat gctgctggtc actggttcgg tcgcaatttc
     1081 gtgaatgcct taggcttcat gtcattcgaa gttggagctt gtctcattgc tggttcgaat
     1141 cgacatgcgc ttgatcatat aagttggcta tctgtactat gcagtgcagg gatctttgca
     1201 acgacgatcc aggcccagga cttcaaggac actgaaggtg atcgacttgt gggccggaaa
     1261 accttaccca ttgttgcgcc gacagttgct cgtcctactc ttctgctcgc gcttttggcg
     1321 tggtctatcg gattaagcac gctttggcag ctcagcacta ctgtagccct tgctttcaat
     1381 gtcctagcca tagcagttgg agcacgtttc gtttcactta agtccatcaa ggcagatcag
     1441 cgcagttttt atctctacaa cgtttggtta tccgtcgcac acgcattacc agcgtatttc
     1501 cgttttatcg ctcaggcacc agtgttctga gcggatctct tatgtcttta cgatttatag
     1561 ttttcattat caagtatgcc tatattagta tatagcatct ttagatgaca gtgttcgaag
     1621 tttcacgaat aaaagataat attctacttt ttgctcccac cgcgtttgct agcacgagtg
     1681 aacaccatcc ctcgcctgtg agttgtaccc attcctctaa actgtagaca tggtagcttc
     1741 agcagtgttc gttatgtacg gcatcctcca acaaacagtc ggttatagtt tgtcctgctc
     1801 ctctgaatcg tctccctcga tatttctcat tttccttcgc atgccagcat tgaaatgatc
     1861 gaagttcaat gatgaaacgg taattcttct gtcatttact catctcatct catcaagtta
     1921 tataattcta tacggatgta atttttcact tttcgtcttg acgtccaccc tataatttca
     1981 attattgaac cctcacgatc cagttctcca gtgacacagc ctttatctgg tcaaaccttt
     2041 ctttctaatc acctatgctg atgcttaatt aagggatttt tgtctccatc aacggcatgc
     2101 gcccaaaaat gacgtttttt ttaacccata gacacgaaac tacccatttt ccaccggcct
     2161 gacctaccac cggaacaacg gccatctcca acttgcaagt tggggaaatt aagagcatcg
     2221 caggtttaat ggaagaaaaa aaaaaggtac agcacagcgc aaatggagtt agttccctta
     2281 tgtcacacac tcacacacag tcggtcagat caagcatact gggtgcgtat aaatagagtg
     2341 gccattgcca ccctgtttat ctcaaaatct gtcttgttag tggtcttctc cctttttcag
     2401 gttacaattc tcttgtttct acttagtata taagtatatc aagctatatt aagcatacta
     2461 tcaactgtca ctctatcctc aaaatacaat acaaaatgga tcccgtactg cgatggatga
     2521 catctgaaca gacgtttacc cctgttatcg gcctcgttgg gctcttctgt agcctcggtc
     2581 ttggattata cgctagtaaa cgagcctata ccccaagcaa acaaagtcta ggacatggtc
     2641 ttccgttgcc tccaggacca aaatcactcc ccatccttgg caatgctctt gatatgccca
     2701 acaagcgtga atgggagaca ttcacaaaat gggcagacga atacggtgat attgtccata
     2761 tcaaggtgtt tggacgtgat attatctaca ttagctctgc caaagttgcg agcgacctgt
     2821 ttgagaaaag atctgctatt tattccgaca ggccacgttt gcctttactt gcagagctga
     2881 tggactttga gtggaacttc gggttcatgc attatggaga cgaatggcga aagaatagac
     2941 gaatgtttgt gagcaagttc ggtccgacga aggttcatac tttcaatcca gtccaagaac
     3001 gtgcttctgg tcttctgctg ggacgacttt tggagactcc cgaggacttc attgaccacc
     3061 tccgtcttca tgctggtcaa ttgataatga tgtccgttta cggtatattt gtcgactcaa
     3121 ggcagcaccc gtatataacg accgctcaag cagtgatgga ctccgtttcc gatgcggcac
     3181 gttctggtgc atatctcgtt gatgcgttcc caatactgaa aagattacca acatggctac
     3241 ctggcacaca ttaccaacgc attgcacgta gatgggcttc cgatgtacga aagctcaggt
     3301 atattccttt ccttgcgagc aagactaaat tggaagatgg tgcaccgccc tatccaacct
     3361 ttgttgctga tatgctagag gagtacggtt acgatgaaaa taacgctgag aatatgatcc
     3421 gggactgtgc agctctcgcg tatggagcgg ggtcggacac gtctgtctcc actttatcgg
     3481 catttatcct cgcaatggtt ctttacccgg acgttcaaag gagaggtcaa gacgagttga
     3541 aggctgtcat tggttcgtct agactcccca cgttcgctga ccgaaaggat ctaccatata
     3601 tcatggcaat tgcgaaggag acgattcgct ggcacacagt tgctccacag gctcttccac
     3661 atgttctcag agaagatgat gtctacaatg gttattatct tccagcgggg tctattgtta
     3721 ttggaaatac atggaaaatt gcgcatgacc ctacagtcta tccagatcca atgaacttca
     3781 agccagaacg ctacttcaat tcagagggtg aactcgacta cgcctccata gatccagcaa
     3841 agtttgcatt tggttacggc agaagaatct gtgcaggata ccattacgct gagaacacgc
     3901 tgtggattac aatcgctttc attttgcagg ttttcagtat tacaaatgca aaagacaagg
     3961 ccggtaatcc tatccctgtt actctaaacg cttcctcggg tgtcatatcc catcccgagc
     4021 cattcccgtg ttcgattatg ccacgctcta cagaagcgga aaccataatt cgccgagccg
     4081 ctgccaatgc caattttgca taaacaaatc gctcttaaat atatacctaa agaacattaa
     4141 agctatatta taagcaaaga tacgtaaatt ttgcttatat tattatacac atatcatatt
     4201 tctatatttt taagatttgg ttatataatg tacgtaatgc aaaggaaata aattttatac
     4261 attattgaac agcgtccaag taactacatt atgtgcacta atagtttagc gtcgtgaaga
     4321 ctttattgtg tcgcgaaaag taaaaatttt aaaaattaga gcaccttgaa cttgcgaaaa
     4381 aggttctcat caactgttta aaaggaggat atcaggtcct atttctgaca aacaatatac
     4441 aaatttagtt tcaaagatga atcagtgcgc gaaggacata actcattcaa ttcaattcat
     4501 catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt gattcggtaa
     4561 tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca
     4621 tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa
     4681 aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta
     4741 ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact
     4801 tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag
     4861 gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg
     4921 gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca
     4981 gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa
     5041 tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg
     5101 gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag
     5161 aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact gttgacattg
     5221 cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag
     5281 atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg
     5341 cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct gacattatta
     5401 ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca
     5461 gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat
     5521 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
     5581 tattacccta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     5641 ataggaaaaa accgagcttc ctttcatccg gcgcggctgt gttctacata tcactgaagc
     5701 tccgggtatt ttaagttata caagggaaag atgccggcta gactagcaag ttttaggctg
     5761 cttaacatta tggataggcg gataaagggc ccaaacagga ttgtaaagct tagacgcttc
     5821 tggttggaca atggtacgtt tgtgtattaa gtaaggcttg gctggggata gcaacattgg
     5881 gcagagtata gaagaccaca aaaaaaaggt atataagggc agagaagtct ttgtaatgtg
     5941 tgtaacttct cttccatgtg taatcagtat ttctacttac ttcttaaata tacagaagta
     6001 agacagataa ccaacagcct ttcccagata tacatatata tctttatttc agcttaaaca
     6061 ataattatat ttgtttaact caaaaataaa aaaaaaaaac caaactcacg caactaatta
     6121 ttccataata aaataacaac atggcaccaa tcataggtgc cgcaggaatt gcttgcaccc
     6181 tatgtttggg tcttttcgtg tatcttagca agcgagctca agcacccagc aaacagagca
     6241 gaggacatgg tcttccgctt ccgcctgggc ctaagccact gccgtttctt gggaatgcac
     6301 ttgatatgcc tgtgaaacgt gaatgggagg catttgccaa atgggcgaat gaatatggtg
     6361 atattgttca tgtcagcgtc ttcggccaga actttatttt cctcaattct gccaaagttg
     6421 tgaacgacct gttcgacaag aggggttcta tctattctga taaacctcgc ttaccattac
     6481 tcacagaatt actagacttt gaatggaact tcggttttat gccctatgga gaggagtgga
     6541 gaaagtatag acgcatgttt gtgggcaagt tcggagctag cgctgctcat atcttcaatc
     6601 caatccatga gcgtgtctcg ggcctattgc tacagcggtt tttggatact ccagaggact
     6661 tcatagatca tcttcgcctc catgcgggtc agattatcat gatgtcgaca tacggtattt
     6721 ttgtagattc aagacagcat ccatatattc aaattgccca agccgtgatg gactcagttt
     6781 ctgatgctgc acgagctggg gcatacctgg tagacgcgtt tccgatcctg aagaaagtac
     6841 caacgtggtt ccctggagcg cattatcagc gtgttgctca gggctgggct gcggaagttc
     6901 gaaggcttag gttcattcct tttcttgcga gcaagaacca attggccgac ggcaaagcgc
     6961 caccttttcc gtcctttgtc gctgatgagc tagaggaaaa cggctatgac gagaaagacg
     7021 ctgaactgat gattcaagat tgtgcggctg tcgcatacgg agcgggatcc gacacttccg
     7081 tcgcaacgct cactgcattc gttctcgcaa tggttattca cccggaaatt caaaagaaag
     7141 ctcaggaaga gcttgcagca gtcgttggaa cttcgaggct ccctacgttc gctgatcgca
     7201 atgatctccc atatatttca gccatcatga aggagacaat acgatggcat acagtagctc
     7261 cgcaggcact cccgcatacc ctcagagaag atgacgtata taatggttat catatcccag
     7321 caggatccat tgttatcggc aatacttgga atattacgca tgacccggca ctctacccag
     7381 accctatgga gttcaagccg gaacgtttta ttaattcaga aggcaaactc gatttttccg
     7441 gcgccacgga cccgtcaaag ttcgcttttg gttatggcag aaggatttgc gccggatggc
     7501 acttcgctga gaatacgata ttcattacaa tagcactcgt tttgcactgt ttcaacatca
     7561 cgaatgcaaa ggataaggct gggatgccca ttcctgtctc tcttaatgct ggttctggtg
     7621 tcatttcgca tcctgagccg ttcccatgtt ccatcactcc acgctctgcc caggcggaag
     7681 ccttaattcg tcaagctgcc gcctccgccc aatttgtcta gagtgctttt aactaagaat
     7741 tattagtctt ttctgcttat tttttcatca tagtttagaa cactttatat taacgaatag
     7801 tttatgaatc tatttaggtt taaaaattga tacagtttta taagttactt tttcaaagac
     7861 tcgtgctgtc tattgcataa tgcactggaa ggagaaaaaa aaggtgcaca cgcgtggctt
     7921 tttcttgaat ttgcagtttg aaaaataact acatggatga taagaaaaca tggagtacag
     7981 tcactttgag aaccttcaat cagctggtaa cgtcttcgtt aattggatac tcaaaaaaga
     8041 tggatagcat gaatcacaag atggaaggaa atgcgggcca cgaccacagt gatatgcata
     8101 tgggagatgg agatgatacc tccattgggc cgatgaagtt agtcgacgga tagaagcggt
     8161 tgtccccttt cccggcgagc cggcagtcgg gccgaggttc ggataaattt tgtattgtgt
     8221 tttgattctg tcatgagtat tacttatgtt ctctttaggt aaccccaggt taatcaatca
     8281 cagtttcata ccggctagta ttcaaattat gacttttctt ctgcagtgtc agccttacga
     8341 cgattatcta tgagctttga atatagtttg ccgtgattcg tatctttaat tggataataa
     8401 aatgcgaagg atcgatgacc cttattatta tttttctaca ctggctaccg atttaactca
     8461 tcttcttgaa agtatataag taacagtaaa atataccgta cttctgctaa tgttatttgt
     8521 cccttatttt tcttttcttg tcttatgcta tagtacctaa gaataacgac tattgttttg
     8581 aactaaacaa agtagtaaaa gcacataaaa gaattaagaa aatggcagcg tacactggtc
     8641 cattacgctc tcgcgcgatg tccttcccta cgaagccagt ccttaaagat ctttttcgtc
     8701 gacgtgcttc cgtgtctctt gattttctcg tcgttggagg cagtatcgca ggtctcgcct
     8761 gtgcgtacaa tctaaaacaa gctgggcata atattgagac gttttgcctt gcatgtgcgc
     8821 tatgtcctcg tgtattgatt caacaggtat ctggaggcat ccgagttccg ccaaatatga
     8881 cgaaacttct tatgcattgg ggattgggtg acagattgat caagactggt gtgcggtgtc
     8941 cctacataga gttcctcgaa agcgataccg gatcgttcct tagttctctg gtgtttcacg
     9001 aagaagtgat gagagagctt caagctgaca tgtatggatt gacagtgagt acaaaaatag
     9061 ctggcgatca tctcgacacg gcactcatcc gacgccccga tcaacagtat tcggatcttt
     9121 attcaatgat atatgacatc acggttgcat cgggagttaa tgtagagtac aacggcgagg
     9181 tggtagatgt ggacgtggac aagccaagtg ttacattggc atcagggcgg aagttacgtg
     9241 cggacattat tgtcggtgca gatggagcaa caagtatagt gcgcgagaaa ttggttggac
     9301 gtaaagaaaa ccatagacca gggccgttaa acggctatac atatactgtc cctgtcagta
     9361 agatgatgga agaccccgaa cttaggccac tagcggagaa caagggagtg tggatagttt
     9421 ggatgggaaa taatacctgc gtcttgacat ttttggttgt aagtttatca actttatcgg
     9481 agatgaagat tgacatacac acatctctaa agcgaccaga cgagtatgcg attcaactga
     9541 ttcatcccac taagaatgcc aggcatgact ggtataagct aacgaatatg gcggcaattg
     9601 tgggtaaatt tgatgactat gagccaagag tgagacgtct tttgcggctt gcaacgcaag
     9661 gtgtcgaaac acggcatatc atacaagaac cgctggaaga ttggtacagt gaaaatggcc
     9721 atgtggtagt ccttggggat gcagcccatg tattgaaccc tggaacgagc catggttctt
     9781 cactggctgt ggaagatgcc gcagttctcg gttctctctt ctctcggttg cgtacacgta
     9841 gctcacgcga aattctgcga ttgctggctg cgtaccaaga aattcgagag gaccgctgta
     9901 tcgaagtcac gagaaccgag tacgaaactt gtgcattttc agttctgccc aggggggatc
     9961 ctagacgcga agagcgagat cttggatttg ctgcctcacg ggatatgaag gttctcgatt
    10021 gggaaaatct cgacgacccg ttcctacaac gcatttggga gcaattcaag ggttctttcg
    10081 gctacgaggc gtacgatgcc gcggatgatt ggtgggtaga ttggggtatt atgcaagaac
    10141 ggatggctgc atcatcaatg gctgttatcg agagctacga gtcctcgcag aacggcgaag
    10201 gcacggatct atacaactaa ggagattgat aagacttttc tagttgcata tcttttatat
    10261 ttaaatctta tctattagtt aattttttgt aatttatcct tatatatagt ctggttattc
    10321 taaaatatca tttcagtatc taaaaattcc cctctttttt cagttatatc ttaacaggcg
    10381 acagtccaaa tgttgattta tcccagtccg attcatcagg gttgtgaagc attttgtcaa
    10441 tggtcgaaat cacatcagta atagtgcctc ttacttgcct catagaattt ctttctctta
    10501 acgtcaccgt ttggtctttt atagtttcga aatctatggt gataccaaat ggtgttccca
    10561 attcatcgtt acgggcgtat tttttaccaa ttgaagtatt ggaatcgtca attttaaagt
    10621 atatctctct tttacgtaaa gcctgcgaga tcctcttaag tatagcgggg aagccatcgt
    10681 tattcgatat tgtcgtaaca aatactttga tcggcgctat gcggccgcca ccgcggtgga
    10741 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgaga ctcaaacaag
    10801 atatattttg cccgccttac agacgaaact agttggaggt tatggagcat actatcacgt
    10861 gggcggccac tggtgagtta ctacacccca ggggcaacgt tgatgctcct aaaaaactct
    10921 ggctggacgc aagccgtaac acccgtgtca cttcataatc gtttgcaatt cagggcttga
    10981 tctacacatt accctgttat ccctattaat taatacggtt atccacagaa tcaggggata
    11041 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    11101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    11161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    11221 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    11281 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11341 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    11401 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11461 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11521 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    11581 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    11641 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11701 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11761 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    11821 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11881 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    11941 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    12001 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    12061 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    12121 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    12181 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    12241 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    12301 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    12361 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    12421 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    12481 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    12541 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12601 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    12661 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    12721 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12781 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12841 catttccccg aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa
    12901 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
    12961 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
    13021 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
    13081 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
    13141 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
    13201 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
    13261 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
    13321 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
    13381 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
    13441 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
    13501 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
    13561 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
    13621 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
    13681 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
    13741 atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
    13801 agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt
    13861 cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct
    13921 gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat
    13981 atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta
    14041 tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg
    14101 tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt
    14161 ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc
    14221 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    14281 cgtttcggtg atgacggtga gcgcgtttcg gtgatgacgg tgaaaacctc ggcgcgccat
    14341 taccctgtta tccctaatat acaacgcaaa gaagctggaa aattggcgag ggaatcctgt
    14401 ttctgtctat ccaagaatgg gcatgaggtg gcaaccgtcg tgctagcgta cagggtgcac
    14461 tttgtaacca tttgggacac cggacactcg ctgttttcga aattaccctt taagcgcggg
    14521 tattgaacca ggcttatgcc caagatcgta gcaagcattt aaatgacggt atcgataagc
    14581 ttgatatcga attcctgcag cccgggg
//
