LOCUS       sequence-223665-       14022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14022)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHCS163.2-2.2
FEATURES             Location/Qualifiers
     source          1..14022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      913..1212
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3845..4065
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4066..4869
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(9650..9670)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9650..9668)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(10007..10595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10087..10106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10766..11557,11558..11626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11389..11408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11627..11731)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11758..13100
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12721..12768)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13141..13159
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13501..13517)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13501..13517)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 acaatgggct tgagtgaaca ggatgttgaa ttcaaaacct tggatggaac tgttcttaga
       61 ggtacactat atcctacaag taatagaggt cccgccatag ttatgtcgcc aggattcaat
      121 tgcgtcaagg aaatgcttgg cctgcctgat gctgcagctc gttttcaatc ggcaggctac
      181 accgtgcttc tttacgatcc aagaaccacg ggtctttcag gcggcgagcc tagaaatgac
      241 atcgacccaa tcgcgcaggt cggcgactac tctgacgcca tcagctacat cagcacgctt
      301 tcctctgtga atccttcaga ggtctttata tggggcatgt ccttctcggc ggcggtgggc
      361 ctttgtgttg ccgcccttga cccaagagcc aagggtatca tcgcagtgtg cccattgaca
      421 gacttcacat acacggagga aaagcttccc atggtgctga aaaagtgcgc ccaggacaga
      481 gctagccagg tcgccggaaa cgatccattt tatttgccca tgctggacgc tcagggcaga
      541 aaccctgcgg gctttggcgt cggcgtggat agcgagcaat actcgaagat tgtcgaggct
      601 ggaaaagaga tcgcccccag acatgtgaac agaacgactg tgcagaccta ctatagaatg
      661 gttttgtggc agccattcgg tttgtggagg atgatcaaaa atcttccagt gttattcgtt
      721 gtgccagagc tggacagatt gagccctgca gaaagacaat tgggtcattg ggactcctta
      781 gtctgcccaa aaagaaaggt cttggtgcca ggcgtgggac atatggagat aatggaaggc
      841 gaccacgtgg atgagataat gactgacatg gagaaattcc tgagagatgt cttggagggt
      901 tcagtaccat aggcggatct cttatgtctt tacgatttat agttttcatt atcaagtatg
      961 cctatattag tatatagcat ctttagatga cagtgttcga agtttcacga ataaaagata
     1021 atattctact ttttgctccc accgcgtttg ctagcacgag tgaacaccat ccctcgcctg
     1081 tgagttgtac ccattcctct aaactgtaga catggtagct tcagcagtgt tcgttatgta
     1141 cggcatcctc caacaaacag tcggttatag tttgtcctgc tcctctgaat cgtctccctc
     1201 gatatttctc attttccttc gcatgccagc attgaaatga tcgaagttca atgatgaaac
     1261 ggtaattctt ctgtcattta ctcatctcat ctcatcaagt tatataattc tatacggatg
     1321 taatttttca cttttcgtct tgacgtccac cctataattt caattattga accctcagga
     1381 tccagttctc cagtgacaca gcctttatct ggtcaaacct ttctttctaa tcacctatgc
     1441 tgatgcttaa ttaagggatt tttgtctcca tcaacggcat gcgcccaaaa atgacgtttt
     1501 ttttaaccca tagacacgaa actacccatt ttccaccggc ctgacctacc accggaacaa
     1561 cggccatctc caacttgcaa gttggggaaa ttaagagcat cgcaggttta atggaagaaa
     1621 aaaaaaaggt acagcacagc gcaaatggag ttagttccct tatgtcacac actcacacac
     1681 agtcggtcag atcaagcata ctgggtgcgt ataaatagag tggccattgc caccctgttt
     1741 atctcaaaat ctgtcttgtt agtggtcttc tccctttttc aggttacaat tctcttgttt
     1801 ctacttagta tataagtata tcaagctata ttaagcatac tatcaactgt caactctatc
     1861 ctcaaaatac aatacaaaat gttggccaag cagatccttt ccaacttgca cttgagtgaa
     1921 gcgttgtatt cagtggtgct agtgctgcta gccggtacct gcgtcttgta tacggcggtt
     1981 ctagcgatct acagattgta ctttagccca ctggctcctt ttcctggacc taagttggca
     2041 gctgtcaccg cgtggtacga gacatacttt gagttggtga agaatggagg tggtcatttc
     2101 acattcgaga tcatgagaat gcatgagaag tacggaccca tcgtgagaat cagcccaaaa
     2161 gagcttcata ttgccgacag tgaattctat gatacgattt acacatcaac gcaaggcttc
     2221 gacaaatctg cacacgttca ggatagattc ggagcgccag aggccgcgtt ttcaactcct
     2281 gatcatgaag tccacaagag acgtcgtgct gctatctccc cattctttgc taaaagacgt
     2341 atccatgagc aagcgcccat gatccaaggc cacgtcaaca agatcgtcag aagacttcat
     2401 cacgaatatg ctggaaaagg aaagattttg aatttgaatg acctttattc gagctatgtg
     2461 tcagatgtaa taatgagcta cgcattcaat cgttcgtacg gttttttgga cgagccagac
     2521 tttgtctcgt ctttcacatc atccattcaa ggattgaaag acttcgtaca ttacgcgcag
     2581 cagcttccct ggttgccaaa gatcattgac agattgcctg acagacttgt cggctacatc
     2641 caacccagca tgaaagccat cctacacttc caaaactcga tgaggaatca ggtgaaggag
     2701 gtgagagaaa acttgcactc tgacaaggcc gaagcccagg aaggtaccat cttcttcagc
     2761 atcatcagat ccgaactacc acccgaagat ttgacgatcc cccgtttgaa ggatgaggcg
     2821 atgagcgttg tcggcgccgg catcgagacg accaagatgg ctttggcagt tctgtgcttt
     2881 cacatcatca acacccccag cgtgttggaa cgtctgcaga atgagctaca agatgccatt
     2941 cccgatccat caaacccccc accactgtct gttttggaga aactgccata tctatatgcg
     3001 tgtctgcagg agggcattcg tctgtcttac ggtgcgatag cacgttcgca aagaatttca
     3061 aggcacaacc ccatcaagta caacgattgg gttatcccac ctggtgttat ggtgtcgcta
     3121 gatacatacc acatgcacca cgacgaggcc gtcttcccac aaagtcacga atacaggcca
     3181 gagagatggc ttggtaatcc cagaggcccc gacggcgaga aggctcttac aaagtatttg
     3241 actgcgtttg gaagaggtac aaggatgtgt gtgggattca acctggccta tgctgagatg
     3301 acgcttgtac ttgcggcact gttcagaagc ttcacctttg agttgtttga gacggacaaa
     3361 ggtgacgtgg agatttttag ggatatgata ggcttggagg ttaagccagg tagtaagggt
     3421 gtcagggtca aagtgagtgg taaaggtgaa gttcagcctt aaacaaatcg ctcttaaata
     3481 tatacctaaa gaacattaaa gctatattat aagcaaagat acgtaaattt tgcttatatt
     3541 attatacaca tatcatattt ctatattttt aagatttggt tatataatgt acgtaatgca
     3601 aaggaaataa attttataca ttattgaaca gcgtccaagt aactacatta tgtgcactaa
     3661 tagtttagcg tcgtgaagac tttattgtgt cgcgaaaagt aaaaatttta aaaattagag
     3721 caccttgaac ttgcgaaaaa ggttctcatc aactgtttaa aaggaggata tcaggtccta
     3781 tttctgacaa acaatataca aatttagttt caaagatgaa tcagtgcgcg aaggacataa
     3841 ctcattcaat tcaattcatc attttttttt tattcttttt tttgatttcg gtttccttga
     3901 aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt
     3961 agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta
     4021 acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca
     4081 tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg
     4141 cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg
     4201 gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg
     4261 actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat
     4321 tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac
     4381 tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg
     4441 ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga
     4501 ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact
     4561 aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa
     4621 agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt
     4681 ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct
     4741 acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag
     4801 gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag
     4861 caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct
     4921 tcaatttaat tatatcagtt attaccctat gcggtgtgaa ataccgcaca gatgcgtaag
     4981 gagaaaatac cgcatcagga taggaaaaaa ccgagcttcc tttcatccgg cgcggctgtg
     5041 ttctacatat cactgaagct ccgggtattt taagttatac aagggaaaga tgccggctag
     5101 actagcaagt tttaggctgc ttaacattat ggataggcgg ataaagggcc caaacaggat
     5161 tgtaaagctt agacgcttct ggttggacaa tggtacgttt gtgtattaag taaggcttgg
     5221 ctggggatag caacattggg cagagtatag aagaccacaa aaaaaaggta tataagggca
     5281 gagaagtctt tgtaatgtgt gtaacttctc ttccatgtgt aatcagtatt tctacttact
     5341 tcttaaatat acagaagtaa gacagataac caacagcctt tcccagatat acatatatat
     5401 ctttatttca gcttaaacaa taattatatt tgtttaactc aaaaataaaa aaaaaaacca
     5461 aactcacgca actaattatt ccataataaa ataacaacat ggacacgttg aagagattgt
     5521 ggcctcagtt gcggcccagc cccagagccc cattgaccga ggccaacctg ccatcgcaag
     5581 cgggcagagt cgtgttggtc acgggcgcca ccgccggcgt cggatacgag ctgtcgagaa
     5641 tcttgtacaa cgcgggcgcc agagtctaca tggccgcgag atcgcccgcc aaagccgacc
     5701 aggccatggc cagaatcaga agcgcccacc ccgcgcccaa ggtgcccggc ggcgccctgg
     5761 tcttcttgca cttggactac gccgacctgg ccaccgtcaa gccatgcgcg ttggcgttcc
     5821 tgtccaagga gccaagactg cacgtcctgt tcaacaatgc cggcatggcc tccgccccac
     5881 ccgaagcgag atcgagcgcc ggcttggagc tgcacatgca ggtcaactgc gtgggcggct
     5941 ttttgctgac caagttgttg acgcctgcgt tggaggctgc tgccaaagac gacgacgcac
     6001 ccccagccgc cgtgagagtc gtcttcagca gcagcatgtt ggtcgacgcc aaggcccccg
     6061 ccgacggcgt cccacccgac gagttggaca acccctcgtt ggacggcgac agaaactacg
     6121 ccatcagcaa ggccgccaac tggttcttgg ccgtgcagtt cgcccagaga ttgggtcccc
     6181 agggcgtcgt ggtcttgacc cagaacccag gcaacctgag aaccgccatc ttcgacgagg
     6241 tgcccaaggc cattgtctgg gcgagctacc cagtgctgtt caagtcgatt gacggcgccc
     6301 acacgtcgct gtggtcgggc ttctcgagcg acgtcaccct ggcagatagc ggcagatacg
     6361 tcgtgccctg gggcaagtgg catccaaacc accgtccaga gcagctgttg gccatcaagg
     6421 acaaaagcga gggcggcaag ggctacgccc aacatctatt tgagtggtgc gacgcgaaga
     6481 ccaaggcgtt ccagtagagt gcttttaact aagaattatt agtcttttct gcttattttt
     6541 tcatcatagt ttagaacact ttatattaac gaatagttta tgaatctatt taggtttaaa
     6601 aattgataca gttttataag ttactttttc aaagactcgt gctgtctatt gcataatgca
     6661 ctggaaggag aaaaaaaagg tgcacacgcg tggctttttc ttgaatttgc agtttgaaaa
     6721 ataactacat ggatgataag aaaacatgga gtacagtcac tttgagaacc ttcaatcagc
     6781 tggtaacgtc ttcgttaatt ggatactcaa aaaagatgga tagcatgaat cacaagatgg
     6841 aaggaaatgc gggccacgac cacagtgata tgcatatggg agatggagat gatacctcca
     6901 ttgggccgat gaagttagtc gacggataga agcggttgtc ccctttcccg gcgagccggc
     6961 agtcgggccg aggttcggat aaattttgta ttgtgttttg attctgtcat gagtattact
     7021 tatgttctct ttaggtaacc ccaggttaat caatcacagt ttcataccgg ctagtattca
     7081 aattatgact tttcttctgc agtgtcagcc ttacgacgat tatctatgag ctttgaatat
     7141 agtttgccgt gattcgtatc tttaattgga taataaaatg cgaaggatcg atgaccctta
     7201 ttattatttt tctacactgg ctaccgattt aactcatctt cttgaaagta tataagtaac
     7261 agtaaaatat accgtacttc tgctaatgtt atttgtccct tatttttctt ttcttgtctt
     7321 atgctatagt acctaagaat aacgactatt gttttgaact aaacaaagta gtaaaagcac
     7381 ataaaagaat taagaaaatg caacctgctt cattgttgct tgccctggct gctgccgctg
     7441 ctgcgtcgcc aacctacggc tcgttccccg tcagaggaca gacgtgtaga aagttgcccc
     7501 aggacaagga ctggccatcg ccacagatct ggaacgtcct gaacgcgacc gtgggaggac
     7561 gtctgattgc cacaacaccc ctagcatctc cctgccacga ccccaactat gatgccgcaa
     7621 agtgtgacgc tttgagagcg ggctgggtgt acccacagac gcatttcgat tcctccacct
     7681 ccattgttgc tccatacttc cagaatcaga gctgcgaccc atttacgtct agagcaagca
     7741 cttgcaaggt tggaacgtac cccagctacg ccatcaaggt caccaaggcc tcggatgcca
     7801 tagcaggcat gcttttcgca caattgaaca atgtgcgtct ggtcatcaag aacaccggtc
     7861 acgacttgtt gggccgttct actggtaccg gaggtctttc gctgtggacg cactacttga
     7921 aggacatcac catcagtgat tacaagtcgt cttcttacac cggcaaggct atgaagatgg
     7981 gtgctggtgt ccaagcctac gaagcctacg aggccgctca caagcagagc ctgagagttg
     8041 tgggtggtac ttgcccaact gttggtttgg ccggtggcta cacccagggt ggtggccact
     8101 cgatgctttc taccgtccac ggtcttggag ctgaccaggt cctggaatgg gaggttgtca
     8161 ccgccaacgg cagcctgttg accgccaccc ccgacaagaa cagcgatctg tactgggccc
     8221 tgtccggtgg cggcggcggt acctacggcc ttgtcttgag catgacggtc aaggcacacg
     8281 ccgacaccaa ggtcaccggc gccatgttga ccatcatcca acagggcgcc gaggacgcct
     8341 tctggaaggg catcacgttg ttccacgcct ccatgacctc gtacacggac gccggcgcgg
     8401 cctttggcta ttacttgggc cagggcatca tggccatcca acccgtcacc ttcccaggca
     8461 agtccaaggc cgaggtcgag gcgttgatgt tggacctgac caagaggttg gatgccgccg
     8521 gtactgtgta ctcgttgacc gtcaccgagt tcagcaacta ctacgacttc ttcaacaact
     8581 actacggccc acttccctac ggcgtctaca cctcggccca ggtccagggt ggacgtctgg
     8641 tcccaagaac agtcgtcgcc accgccgcct ccaaccaggc cttcaccgac gccttgaagg
     8701 ccatcgtcca ggaccaggac ttccacgtcg tcggcgtcgg cttggacgtc agcaagacag
     8761 caaccgcttc caacgccgtg caccccaagt ggagatcgac cgccatcacc atcgtcgtcg
     8821 ccgccaactg ggactacacg cagccatttg cgaccaacgc cgccaacgaa accttgatca
     8881 ccgacaagta cgacccattg ttgacggccg tcacgggcgc cgacagcggc gcctacgtca
     8941 acgagggcga cttccagcag cccgcgttcc aggacaactt tttcggtgca aactacgcca
     9001 agctgagatc catcaagaag aagtacgacc ccaacagctt gttctacgcg accaagctgg
     9061 tgggctcgga agactgggct gtgcaggctg atggcaggct ttgcaaggcc tgaggagatt
     9121 gataagactt ttctagttgc atatctttta tatttaaatc ttatctatta gttaattttt
     9181 tgtaatttat ccttatatat agtctggtta ttctaaaata tcatttcagt atctaaaaat
     9241 tcccctcttt tttcagttat atcttaacag gcgacagtcc aaatgttgat ttatcccagt
     9301 ccgattcatc agggttgtga agcattttgt caatggtcga aatcacatca gtaatagtgc
     9361 ctcttacttg cctcatagaa tttctttctc ttaacgtcac cgtttggtct tttatagttt
     9421 cgaaatctat ggtgatacca aatggtgttc ccaattcatc gttacgggcg tattttttac
     9481 caattgaagt attggaatcg tcaattttaa agtatatctc tcttttacgt aaagcctgcg
     9541 agatcctctt aagtatagcg gggaagccat cgttattcga tattgtcgta acaaatactt
     9601 tgatcggcgc tatgcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     9661 ggttaattgc gcgcttggcg agactcaaac aagatatatt ttgcccgcct tacagacgaa
     9721 actagttgga ggttatggag catactatca cgtgggcggc cactggtgag ttactacacc
     9781 ccaggggcaa cgttgatgct cctaaaaaac tctggctgga cgcaagccgt aacacccgtg
     9841 tcacttcata atcgtttgca attcagggct tgatctacac attaccctgt tatccctatt
     9901 aattaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9961 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10021 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10081 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10141 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10201 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10261 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10321 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10381 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10441 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10501 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10561 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10621 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10681 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10741 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10801 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10861 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10921 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10981 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    11041 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    11101 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    11161 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    11221 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    11281 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    11341 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    11401 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11461 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11521 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11581 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac
    11761 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
    11821 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
    11881 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt
    11941 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
    12001 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
    12061 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
    12121 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
    12181 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
    12241 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
    12301 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
    12361 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
    12421 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
    12481 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
    12541 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
    12601 ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg
    12661 tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct
    12721 gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg
    12781 aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc
    12841 gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt
    12901 gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt
    12961 acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc
    13021 tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc
    13081 atttcctttg atattggatc atactaagaa accattatta tcatgacatt aacctataaa
    13141 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgagcgcgtt
    13201 tcggtgatga cggtgaaaac ctcggcgcgc cattaccctg ttatccctaa tatacaacgc
    13261 aaagaagctg gaaaattggc gagggaatcc tgtttctgtc tatccaagaa tgggcatgag
    13321 gtggcaaccg tcgtgctagc gtacagggtg cactttgtaa ccatttggga caccggacac
    13381 tcgctgtttt cgaaattacc ctttaagcgc gggtattgaa ccaggcttat gcccaagatc
    13441 gtagcaagca tttaaatgac ggtatcgata agcttgatat cgaattcctg cagcccgggg
    13501 gatccactag ttctagatta attaatatct aaaaattgcc ttatgatccg tctctccggt
    13561 tacagcctgt gtaactgatt aatcctgcct ttctaatcac cattctaatg ttttaattaa
    13621 gggattttgt cttcattaac ggctttcgct cataaaaatg ttatgacgtt ttgcccgcag
    13681 gcgggaaacc atccacttca cgagactgat ctcctctgcc ggaacaccgg gcatctccaa
    13741 cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaaa
    13801 aaaggcagag gagagcatag aaatggggtt cactttttgg taaagctata gcatgcctat
    13861 cacatataaa tagagtgcca gtagcgactt ttttcacact cgaaatactc ttactactgc
    13921 tctcttgttg tttttatcac ttcttgtttc ttcttggtaa atagaatatc aagctacaaa
    13981 aagcatacaa tcaactatca actattaact atatcgtaat ac
//
