LOCUS       sequence-223682-        5941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Wang X, Srivastava Y, Jankowski A, Malik V, Wei Y, Del Rosario RC, 
            Cojocaru V, Prabhakar S, Jauch R
  TITLE     DNA-mediated dimerization on a compact sequence signature controls 
            enhancer engagement and regulation by FOXA1.
  JOURNAL   Nucleic Acids Res. 2018 Jun 20;46(11):5470-5486. doi: 
            10.1093/nar/gky259.
  PUBMED    29669022
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-06-20- 20"; volume: "46"; issue: 
            "11"; pages: "5470-5486"
COMMENT     Sequence Label: ESR1-Tol2
FEATURES             Location/Qualifiers
     source          1..5941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(509..696,697..708)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(720..739)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(721..739)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(746..763)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(746..762)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(755..777)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      903..1358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(990..1009)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1200..1221
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1436..1540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1541..1609,1610..2401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1759..1778)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2572..3160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3061..3080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3314..3331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3448..3469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3484..3489,3490..3507,3508..3514)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3522..3538
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3527..3549
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3546..3562
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3546..3562
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3580..3598
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3580..3597
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(3649..3660,3661..3823)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             join(4904..4906,4907..4909,4910..5623)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4904..4906,4907..4909,4910..5620)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4949..4970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5210..5229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5557..5578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5746..5867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5783..5802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5837..5856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aactatcagt aaaacaaaat acttaatgta
       61 cttacctcaa tatgcttcct taggtttgat ggcgaacttt tgaaggccga tatttcttta
      121 ttaagcggga gacagaggac acatttcatc ttgaatgaat ctttatttac accacttaaa
      181 gaaaagaatt cgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact
      241 gtgctgctcg cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact
      301 gttataattt ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat
      361 gacgtcactt ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga
      421 ttttcatagg cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg
      481 tttggtaata gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac
      541 tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta
      601 aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata
      661 cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggg cccaattcgc
      721 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      781 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      841 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      901 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      961 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1021 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1081 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1141 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1201 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     1261 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1321 taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
     1381 acgcatctgt gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg
     1441 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1501 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1561 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1621 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1681 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1741 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     1801 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1861 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1921 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1981 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2041 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2101 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2161 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2221 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2281 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2341 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2401 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2461 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2521 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2581 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2641 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2701 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2761 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2821 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2881 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2941 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3001 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3061 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3121 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3181 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3241 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3301 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     3361 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     3421 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     3481 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     3541 tcacacagga aacagctatg accatgatta cgccaagcta tttaggtgac actatagaat
     3601 actcaagcta tgcatccaac gcgttgggag ctctcccata tggtcgagca gaggtgtaaa
     3661 aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat
     3721 taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta
     3781 aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct
     3841 tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct
     3901 ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg
     3961 ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt
     4021 agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtcgacg tcggccatag
     4081 cggccgcgga agattttagc catccaagaa aggtaccttg tacttctaag caattctccc
     4141 atcacctgga cgttaactgc agcccacact gcagtgtctt gactgcagta ggcactcagt
     4201 aaatatttat tgatggtcct gattatggga tgatgaaata tgctaaaggt aacttaaact
     4261 tttgtcaata aaaccgagtt tcaggttagt ttgacctttt ccgtatctct aaatattttc
     4321 tttcatgtca tttttgtgaa atagctcacg aaagtactta ttgattctga aatccttttc
     4381 ccctctggta ttcctgagct atcctcactc acacacagca caccagtgct attgtttgct
     4441 gttttgttaa aatcattctc attaatattc ctcattcaat aaaaatagtt gattcccact
     4501 tatgcaccaa actctattct aaagactgct gtttatttta tggagtcaat atcatcattt
     4561 tgttttccat tccacataat agttggatgc caatatgaag tgtatattta taaataatat
     4621 gtgcttttat ttatttgttt atttttattt tactttaagt tctgggatta atatgtgctt
     4681 ttaacattat tgtttaaaaa taacaggaga tatcatatct taagcacctt ctatattctg
     4741 ggcactgttc taaatgtatc aaagttatca tctcatttaa tccctccaca cacctgagcg
     4801 cggatccccc agtgacgtag gaagtccatc cattcacagc gcttctataa aggcgccagc
     4861 tgaggcgcct actactccaa ccgcgactgc agcgagcaac tccatggtga gcaagggcga
     4921 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4981 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     5041 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     5101 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     5161 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     5221 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     5281 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     5341 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     5401 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     5461 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     5521 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     5581 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     5641 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     5701 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     5761 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5821 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     5881 aagcgttaat cctactacac atataaaagg cgcgccatca acgtcaaaac atgcacaccc
     5941 a
//
