LOCUS       sequence-223788-        8478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of mRuby2 and mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW mRuby2-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      841..850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             847..1557
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             1564..1617
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1633..1642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1639..1641,1642..1644,1645..2358)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1684..1705)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1945..1964)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2292..2313
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2368..2392)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2454..2473)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2455..2473)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2621..2808
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2676..2700)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2820..2837)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2820..2836)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2829..2851)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3272..4618
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4729..5532)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5533..5748)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5773..5791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5859..5963
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5964..6032,6033..6824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6182..6201)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6995..7583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7484..7503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7737..7754
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7871..7892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7907..7912,7913..7930,7931..7937)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7945..7961
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7950..7972
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7969..7985
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7969..7985
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8015..8470)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8152..8173)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8364..8383
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc gctaccggtc
      841 gccaccatgg tgtctaaggg cgaagagctg atcaaggaaa atatgcgtat gaaggtggtc
      901 atggaaggtt cggtcaacgg ccaccaattc aaatgcacag gtgaaggaga aggcaatccg
      961 tacatgggaa ctcaaaccat gaggatcaaa gtcatcgagg gaggacccct gccatttgcc
     1021 tttgacattc ttgccacgtc gttcatgtat ggcagccgta cttttatcaa gtacccgaaa
     1081 ggcattcctg atttctttaa acagtccttt cctgagggtt ttacttggga aagagttacg
     1141 agatacgaag atggtggagt cgtcaccgtc atgcaggaca ccagccttga ggatggctgt
     1201 ctcgtttacc acgtccaagt cagaggggta aactttccct ccaatggtcc cgtgatgcag
     1261 aagaagacca agggttggga gcctaataca gagatgatgt atccagcaga tggtggtctg
     1321 aggggataca ctcatatggc actgaaagtt gatggtggtg gccatctgtc ttgctctttc
     1381 gtaacaactt acaggtcaaa aaagaccgtc gggaacatca agatgcccgg tatccatgcc
     1441 gttgatcacc gcctggaaag gttagaggaa agtgacaatg aaatgttcgt agtacaacgc
     1501 gaacacgcag ttgccaagtt cgccgggctt ggtggtggga tggacgagct gtacaagtcc
     1561 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctggg
     1621 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1681 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1741 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1801 cccgtgccct ggcccaccct cgtgaccacc ctgtcctggg gcgtgcagtg cttcgcccgc
     1861 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1921 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1981 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2041 ggcaacatcc tggggcacaa gctggagtac aactacttta gcgacaacgt ctatatcacc
     2101 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
     2161 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2221 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
     2281 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2341 gacgagctgt acaagtaaag cggccgcacc cagctttctt gtacaaagtg gtgtagttaa
     2401 ttcatgatgg ccgctgcagg tcgacctcga gggggggccc ggtacccaat tcgccctata
     2461 gtgagtcgta ttacgcgcgg atccagcttt ggacttcttc gccagaggtt tggtcaagtc
     2521 tccaatcaag gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg
     2581 tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat
     2641 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
     2701 ttaggtttta aaacganaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
     2761 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccaattca
     2821 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2881 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     2941 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     3001 acgcatctgt gcggtatttc acaccgcata atcggatcgt acttgttacc catcattgaa
     3061 ttttgaacat ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata
     3121 ataatatata gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac
     3181 tattgcatct attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt
     3241 ccatcttgca cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga
     3301 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     3361 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
     3421 gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     3481 tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
     3541 tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
     3601 actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
     3661 gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
     3721 tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
     3781 aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa
     3841 gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
     3901 ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
     3961 aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat
     4021 gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag
     4081 ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt
     4141 attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg
     4201 tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag
     4261 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
     4321 caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
     4381 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
     4441 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
     4501 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
     4561 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcga
     4621 tccgatgata agctgtcaaa catgagaatt gggtaataac tgatataatt aaattgaagc
     4681 tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg
     4741 gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt
     4801 agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga
     4861 gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc
     4921 cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg
     4981 agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt
     5041 atattctcca gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct
     5101 aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac
     5161 cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta
     5221 ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt
     5281 gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa
     5341 gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag
     5401 taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat
     5461 gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt
     5521 agctttcgac atgatttatc ttcgtttcct gcatgttttt gttctgtgca gttgggttaa
     5581 gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag
     5641 tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca
     5701 aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctaattctt
     5761 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5821 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5881 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5941 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6001 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6061 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6121 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6181 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6241 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6301 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6361 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6421 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6481 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6541 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6601 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6661 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6721 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6781 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6841 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6901 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6961 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7021 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7081 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7141 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7201 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7261 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7321 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7381 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7441 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7501 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7561 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7621 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7681 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7741 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7801 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7861 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7921 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7981 atgaccatga ttacgccaag cttaccgcat caggaaattg taagcgttaa tattttgtta
     8041 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     8101 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     8161 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     8221 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     8281 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     8341 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     8401 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     8461 cagggcgcgt ccattcgc
//
