LOCUS       sequence-223792-        8473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of mTq2 and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW mTurquoise2-T2A-mcherry
FEATURES             Location/Qualifiers
     source          1..8473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      841..850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(847..849,850..852,853..1563)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(892..913)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1153..1172)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1500..1521
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1570..1623
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1645..2355
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1790..1808)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2058..2077
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2363..2387)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2449..2468)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2450..2468)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2616..2803
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2671..2695)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2815..2832)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2815..2831)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2824..2846)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3267..4613
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4724..5527)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5528..5743)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5768..5786)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5854..5958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5959..6027,6028..6819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6177..6196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6990..7578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7479..7498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7732..7749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7866..7887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7902..7907,7908..7925,7926..7932)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7940..7956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7945..7967
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7964..7980
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7964..7980
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8010..8465)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8147..8168)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8359..8378
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc gctaccggtc
      841 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      901 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      961 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1021 cccaccctcg tgaccaccct gtcctggggc gtgcagtgct tcgcccgcta ccccgaccac
     1081 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1141 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1201 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1261 gggcacaagc tggagtacaa ctactttagc gacaacgtct atatcaccgc cgacaagcag
     1321 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     1381 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1441 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     1501 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1561 aagtccggag agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     1621 cctggggatc caccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc
     1681 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     1741 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1801 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1861 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     1921 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1981 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     2041 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     2101 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     2161 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     2221 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     2281 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     2341 gagctgtaca agtagcggcc gcacccagct ttcttgtaca aagtggtgta gttaattcat
     2401 gatggccgct gcaggtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag
     2461 tcgtattacg cgcggatcca gctttggact tcttcgccag aggtttggtc aagtctccaa
     2521 tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa
     2581 tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt
     2641 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     2701 ttttaaaacg anaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     2761 aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgcca attcactggc
     2821 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2881 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2941 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     3001 tctgtgcggt atttcacacc gcataatcgg atcgtacttg ttacccatca ttgaattttg
     3061 aacatccgaa cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat
     3121 atatagtcta gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg
     3181 catctattgc ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc
     3241 ttgcacttca atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa
     3301 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
     3361 agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa
     3421 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     3481 agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt
     3541 gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt
     3601 ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg
     3661 ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact
     3721 ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg
     3781 catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat
     3841 agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta
     3901 tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag
     3961 ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga
     4021 ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata
     4081 tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg
     4141 caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc
     4201 agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctagc tagagaatag
     4261 gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg
     4321 cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat
     4381 atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg
     4441 cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat
     4501 gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc
     4561 tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg atcgatccga
     4621 tgataagctg tcaaacatga gaattgggta ataactgata taattaaatt gaagctctaa
     4681 tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc
     4741 atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat
     4801 cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca
     4861 catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac
     4921 cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa
     4981 taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt
     5041 ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt
     5101 cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac
     5161 cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca
     5221 atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact
     5281 tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat
     5341 ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt
     5401 ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat
     5461 taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt
     5521 tcgacatgat ttatcttcgt ttcctgcatg tttttgttct gtgcagttgg gttaagaata
     5581 ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt
     5641 gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa
     5701 accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaaaagctaa ttcttgaaga
     5761 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5821 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5881 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5941 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6001 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6061 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6121 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6181 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6241 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6301 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6361 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6421 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6481 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6541 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6601 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6661 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6721 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6781 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6841 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6901 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6961 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7021 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7081 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7141 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7201 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7261 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7321 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7381 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7441 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7501 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7561 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7621 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7681 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7741 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7801 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7861 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7921 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7981 catgattacg ccaagcttac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     8041 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     8101 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     8161 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     8221 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     8281 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     8341 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     8401 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     8461 cgcgtccatt cgc
//
