LOCUS       sequence-223801-        9190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of tdTomato and mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW tdTomato-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..9190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             842..2269
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             2276..2329
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2345..2354
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2351..2353,2354..2356,2357..3070)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2396..2417)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2657..2676)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3004..3025
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3080..3104)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3166..3185)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3167..3185)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3333..3520
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3388..3412)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3532..3549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3532..3548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3541..3563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3984..5330
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5441..6244)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6245..6460)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6485..6503)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6571..6675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6676..6744,6745..7536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6894..6913)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7707..8295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8196..8215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8449..8466
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8583..8604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8619..8624,8625..8642,8643..8649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8657..8673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8662..8684
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8681..8697
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8681..8697
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8727..9182)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8864..8885)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9076..9095
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc gctaccggtc
      841 gatggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga
      901 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      961 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     1021 catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat
     1081 ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     1141 cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat
     1201 ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     1261 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     1321 cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac
     1381 catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct
     1441 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg
     1501 ccgccaccac ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac
     1561 cgcctcctcc gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg
     1621 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1681 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     1741 ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc
     1801 cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1861 gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac
     1921 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
     1981 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     2041 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     2101 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac
     2161 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     2221 cgagggccgc caccacctgt tcctgtacgg catggacgag ctgtacaagt ccggagaggg
     2281 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctg gggatccacc
     2341 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2401 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2461 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2521 ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga
     2581 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2641 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2701 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2761 cctggggcac aagctggagt acaactactt tagcgacaac gtctatatca ccgccgacaa
     2821 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
     2881 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2941 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga
     3001 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3061 gtacaagtaa agcggccgca cccagctttc ttgtacaaag tggtgtagtt aattcatgat
     3121 ggccgctgca ggtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg
     3181 tattacgcgc ggatccagct ttggacttct tcgccagagg tttggtcaag tctccaatca
     3241 aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg
     3301 ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta
     3361 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     3421 taaaacgana attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     3481 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgccaatt cactggccgt
     3541 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3601 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3661 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     3721 gtgcggtatt tcacaccgca taatcggatc gtacttgtta cccatcattg aattttgaac
     3781 atccgaacct gggagttttc cctgaaacag atagtatatt tgaacctgta taataatata
     3841 tagtctagcg ctttacggaa gacaatgtat gtatttcggt tcctggagaa actattgcat
     3901 ctattgcata ggtaatcttg cacgtcgcat ccccggttca ttttctgcgt ttccatcttg
     3961 cacttcaata gcatatcttt gttaacgaag catctgtgct tcattttgta gaacaaaaat
     4021 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga
     4081 aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca
     4141 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     4201 acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt
     4261 ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt
     4321 tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta
     4381 aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca
     4441 cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat
     4501 ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc
     4561 gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat
     4621 actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc
     4681 ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt
     4741 cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag
     4801 cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa
     4861 tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga
     4921 gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagctag agaataggaa
     4981 cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga
     5041 gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata
     5101 tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt
     5161 ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg
     5221 gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca
     5281 attggattag tctcatcctt caatgctatc atttcctttg atattggatc gatccgatga
     5341 taagctgtca aacatgagaa ttgggtaata actgatataa ttaaattgaa gctctaattt
     5401 gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc
     5461 ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc
     5521 ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat
     5581 catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg
     5641 gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa
     5701 agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc
     5761 cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct
     5821 ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt
     5881 gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt
     5941 tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg
     6001 cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca
     6061 catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct
     6121 tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa
     6181 atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg
     6241 acatgattta tcttcgtttc ctgcatgttt ttgttctgtg cagttgggtt aagaatactg
     6301 ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct
     6361 ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaggaaacc
     6421 gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa aagctaattc ttgaagacga
     6481 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     6541 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6601 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6661 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6721 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6781 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6841 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6901 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6961 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7021 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7081 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7141 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7201 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7261 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7321 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7381 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7441 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7501 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7561 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7621 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7681 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7741 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7801 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7861 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7921 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7981 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8041 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8101 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8161 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8221 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8281 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8341 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8401 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8461 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8521 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     8581 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8641 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     8701 gattacgcca agcttaccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc
     8761 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     8821 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     8881 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     8941 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     9001 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     9061 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     9121 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     9181 gtccattcgc
//
